rev:   November 18, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


NEUROPeptide Y  & Receptors (see below 3 related polyclonals)
Polyclonal Antibody to Neuropeptide Y 1-20

Cat#RDI-PRO16056    $438.00    

Category                     Rabbit polyclonal

Form/Purification        Undiluted serum (lyoph.)

Immunogen                   Synthetic cys(21)-NPY 1-21, conjugated to BSA

Description/Specificity:   Neuropeptide Y is a peptide belonging to the PP-family and occurs in neurons and adrenal medullary cells. The brain contains large quantities of NPY and it is also found in the peripheral nervous system, where it coexists with noradrenaline in sympathetic fibers. NPY inhibits gut motility and causes vasoconstriction. Pheochromocytomas contain NPY.

  Absorption with 10-100 µg NPY 1-20 per ml diluted antiserum abolishes the staining, while PYY and PP do not.

Positive Control Stefanini-fixed sections of rat small intestine

Species tested so far Human, rat

Application · Immunofluorescence · Suitable for frozen sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and  0.1% NaN3.

Working Dilution 1:200 - 1:400 for immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

For In Vitro Research Use Only-not for use in diagnostics


Polyclonal Antibody to Neuropeptide Y

Cat#RDI-PRO16054  $438.00  

Category                    Rabbit polyclonal

Form/Purification      Undiluted serum (lyoph.)

Immunogen                Synthetic neuropeptide Y (Peninsula), conjugated to BSA

Description/Specificity Neuropeptide Y (NPY) is a peptide belonging to the PP-family and occurs in neurons and adrenal medullary cells. The brain contains large quantities of NPY and it is also found in the peripheral nervous system, where it coexists with noradrenaline in sympathetic fibers. NPY inhibits gut motility and causes vasoconstriction. Pheochromocytomas contain NPY.

Absorption with 10-100 µg NPY per ml diluted antiserum abolishes the staining, while PYY and PP do not.

Positive Control Stefanini-fixed sections of rat small intestine

Species tested so far Human, rat, guinea pig

Application · Immunofluorescence · Immunohistochemistry · Suitable for frozen and paraffin sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:300 - 1:600 (PAP)

                           1:200 - 1:400 (immunofluorescence microscopy) with overnight incubation at 2-8°C.

Storage At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

Reference Y et al. Immunohistochemical studies on the distribution of nerve fibres in chronic liver diseases. Am J Gastroenterology 83, 1108-1114 (1988)

For Research Use Only-Nor for use in diagnostics


Polyclonal Antibody to Prepro-Neuropeptide Y (C-PON)

Cat#RDI-PRO16057  $625.00

Category                   Rabbit polyclonal

Form/Purification     Undiluted serum (lyoph.)

Immunogen               Synthetic Prepro-NPY 68-97 (C-PON)

Description/Specificity Neuropeptide Y is a peptide belonging to the PP-family and occurs in neurons and adrenal medullary cells. Antisera raised against NPY often cross-react with PP and PYY. This antiserum was raised using a synthetic peptide from the prepro-NPY sequence which has no homologies to PP and NPY.

Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.

Positive Control Stefanini-fixed sections of rat small intestine

Species tested so far         Human, rat

Application · Immunofluorescence  · Suitable for frozen sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:400 - 1:800 for immunofluorescence microscopy with overnight incubation at 2-8°C.

Storage At 2-8°C (lyoph.) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

For Research Use Only-not for use in Diagnostics


Rabbit anti-Neuropeptide Y 1 Receptor (NPY1R) Polyclonal Antibody

(IgG fraction) Mouse/Rat/Human/Dog/Chicken

cat# RDI-NYPY1RCabrX   $469.00/vial

Presentation: 100ug affinity pure antibody lyophilized from a 0.1ml (1mg/ml) 100mM tris pH7.5 stock with 0.2% BSA and 0.05% sodium azide).

Reconsitution: add at least 100ul sterile DI water , tighten cap,lightly vortex. allow to set at least 30 minutes at room temp. If desired, centrifuge for 1 minute at 1000rpm to concentrate antibody in vial. Recommend making any further dilutions in buffer with 0.1-1% BSA or other carrier and 0.05% sodium azide.

Purification: peptide affinity purified.

Antigen: A 14 amino acid peptide sequence derived from mouse sequence (near c terminal)

Reconstitution: All peptide/antibody received in solution or reconstituted from Lyophilized vials should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw.

Specificity: The antigen has 100% sequence homology to rat and 93% homology to human, dog and chicken.

USE: Western Blotting (1-10 ug/ml for affinity pure using Chemiluminescence technique).

ELISA (1:10K; using 50-100 ng of control peptide/well).

Histochemistry & Immunofluorescence:We recommend the testing of affinity purified antibody at 2- 20ug/ml on acetone fixed frozen sections or pfa fixed tissues.

Background: Neuropeptide Y (NPY), a neuropeptide colocalized and abundantly released with the neurotransmitter norepinephrine in nervous system, synthesized by postnatal olfactory epithelium of sustentacular cells. It promotes the proliferation of postnatal neuronal precursor cells and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the  cardiovascular system.

NPY a 97aa peptide in mouse, human (chr 7p15.1) and 98aa in rat has a mol. wt of 11kD. NPY shows sequence homology to Peptide YY (PYY) and over 50% homology in amino acid and nucleotide sequence to Pancreatic Polypeptide (PNP or PPY), all of these homologous peptides has a 36aa mature peptide characterized by a hairpin loop.

NPY1R, a 382aa in mouse, rat and 384aa in human (chr 4q31.3) mainly expressed in brain, heart, kidney and spleen. It is the receptor for Neuropeptide Y and Peptide YY.

control/blocking peptide: cat#RDI-NYP1RC-CP $188.00/100ug

control peptides are provided lyophilized from 1 mg/ml solution (100 µg/100 µl) in PBS + 0.05% sodium azide. Add 100ul DI water, let set at leats 30 minutes, gently mix (can then centrifuge 1 minute 1000rpm to concentrate material in vial). Generally titer antibody to maximum usable dilution and then pretreat antibody with 10X weight of peptide for 2-3 hours RT or overnight 4 DEG C, then proceed with staining. Blocking petides are too small generally <3KDa so cannot be run on western blot.

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS

see also antibodies to NYP and receptors NYP1R, NYP2R, NYP3R, NYP4R, NYP5R, NYP6R


Rabbit anti-Neuropeptide Y 1 Receptor (NPY1R) Polyclonal Antibody

(IgG fraction) Mouse Rat/Human ?

cat# RDI-NYPY1RNabrX    $469.00/vial

Presentation: 100ug affinity pure antibody lyophilized from a 0.1ml (1mg/ml) 100mM tris pH7.5 stock with 0.2% BSA and 0.05% sodium azide).

Reconsitution: add at least 100ul sterile DI water , tighten cap,lightly vortex. allow to set at least 30 minutes at room temp. If desired, centrifuge for 1 minute at 1000rpm to concentrate antibody in vial. Recommend making any further dilutions in buffer with 0.1-1% BSA or other carrier and 0.05% sodium azide.

Purification: peptide affinity purified.

Antigen: A 15 amino acid peptide sequence derived from mouse sequence (near N terminal)

Reconstitution: All peptide/antibody received in solution or reconstituted from Lyophilized vials should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw.

Specificity: The antigen has 86% homology to human and 80% to rat.

USE: Western Blotting (1-10 ug/ml for affinity pure using Chemiluminescence technique).

ELISA (1:10K; using 50-100 ng of control peptide/well).

Histochemistry & Immunofluorescence:We recommend the testing of affinity purified antibody at 2- 20ug/ml on acetone fixed frozen sections or pfa fixed tissues.

Background: Neuropeptide Y (NPY), a neuropeptide colocalized and abundantly released with the neurotransmitter norepinephrine in nervous system, synthesized by postnatal olfactory epithelium of sustentacular cells. It promotes the proliferation of postnatal neuronal precursor cells and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the  cardiovascular system.

NPY a 97aa peptide in mouse, human (chr 7p15.1) and 98aa in rat has a mol. wt of 11kD. NPY shows sequence homology to Peptide YY (PYY) and over 50% homology in amino acid and nucleotide sequence to Pancreatic Polypeptide (PNP or PPY), all of these homologous peptides has a 36aa mature peptide characterized by a hairpin loop.

NPY1R, a 382aa in mouse, rat and 384aa in human (chr 4q31.3) mainly expressed in brain, heart, kidney and spleen. It is the receptor for Neuropeptide Y and Peptide YY.

control/blocking peptide: cat#RDI-NYP1RN-CP $188.00/100ug

control peptides are provided lyophilized from 1 mg/ml solution (100 µg/100 µl) in PBS + 0.05% sodium azide. Add 100ul DI water, let set at leats 30 minutes, gently mix (can then centrifuge 1 minute 1000rpm to concentrate material in vial). Generally titer antibody to maximum usable dilution and then pretreat antibody with 10X weight of peptide for 2-3 hours RT or overnight 4 DEG C, then proceed with staining. Blocking petides are too small generally <3KDa so cannot be run on western blot.

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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