rev: January 21, 2005
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Myelin Basic Protein (see 8 different antibodies below + pure antigen)
cat# RDI-MBPabm-P12 $594.00
Presentation: 100ug of protein A purified IgG2a in 100ul of PBS, pH 7.4, 0.1% sodium azide and 30% glycerol. Liquid at -20 C.
Immunogen: Synthetic peptide corresponding to the human myelin basic protein
sequence phosphorylated at Thr98 and coupled to tuberculin.
Clone: P12
Isotype: IgG2a
Uses: -Immunoblot Analysis: 0.5-2ug/ml of this lot strongly detected
phosphorylated MBP. Note: Some preparations of MBP contain basal levels of
phosphorylated MBP, which are detected by the pMBP antibody.
-Immunoprecipitation: Not tested.
-Immunocytochemistry: Please refer to reference 3.
-Protein Kinase Assay:
References:
1. Yon, M., et al., J. Neuroimmuno. 58: 121-129, 1995.
2. Yon, M., et al., J. Neuroimmuno. 65: 55-59, 1996.
3. Mandell, JW and Gocan, N.C., Anal. Biochem. 293: 264-268, 2001.
Storage: Stable for 2 years at -20 C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN HUMANS OR ANIMALS
Quality Control Testing Additional Research Applications
Immunoblot Protocol
1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a cell lysate sample (cell lysis buffer: 50mM Tris-HCl, pH7.4; 1% NP-40; 0.25% sodium deoxycholate; 150mM NaCl; 1mM EGTA; 1mM PMSF; 1ug/ml each aprotinin, leupeptin, pepstatin; 1mM Na3VO4; 1mM NaF) and transfer the proteins to nitrocellulose. Wash the blotted nitrocellulose twice with water.
2. Block the blotted nitrocellulose in freshly prepared 3% nonfat dry milk in TBS (TBS-MLK) for 20 minutes at room temperature with constant agitation.
3. Incubate the nitrocellulose with 0.5-2ug/ml of anti-phospho-MBP, clone P12 diluted in freshly prepared TBS-MLK overnight with agitation at 4°C.
4. Wash the nitrocellulose twice with water.
5. Incubate the nitrocellulose in the secondary reagent of choice in TBS-MLK for 1.5 hours at room temperature with agitation.
6. Wash the nitrocellulose with water twice.
7. Wash the nitrocellulose in TBS-0.05% Tween 20 for 3-5 minutes.
8. Rinse the nitrocellulose in 4-5 changes of water.
9. Use detection method of choice (enhanced chemiluminescence was used).
Catalogue # RDI-TRK4M79-BM15 $438.00/1mg $406.00/mg 2-9
Clone: BM15
Mouse isotype: IgG2a
Reactivity: The antibodies recognize 18.5 kDa isoform of MBP
Application: The antibodies are working in ELISA and Western Blotting.
Purification: Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.
Presentation: Each vial contains antibodies in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at + 4 °C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
For Research Use Only
-Reacts with human, bovine, rabbit, guinea pig, rat and sheep MBP
-Cytochemical staining
-immunoblotting
-Elisa
Ref: -Groome, NP et al (1985) Neurochemistry Int 7 pp309-317
-Groome, NP et al (1988) J. Neuroimmunol 12 pp
253-264
Storage: Store at 4 DEG C. -20 DEG C Long term AVOID FREQUENT FREEZE
THAW CYCLES.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Catalog#: RDI-MBPabm-22
Cost: $500.00/vial $438.00/vial 2 or more
clone (isotype) 22 (mIgG2b)
Packaging: 2ml TCS and 0.09% sodium azide
Species: mouse
Source: tissue culture supernatant
Purification Method: none
Antigen: Human myelin basic Protein
Specificity: Approx epitope between aa 84-89 (Martensen numbering) sequence
D E N P U U
-Reacts with human and most species
-Cytochemical staining
-immunoblotting
-Elisa
Ref: -Groome, NP et al (1985) Neurochemistry Int 7 pp309-317
-Groome, NP et al (1988) J. Neuroimmunol 12 pp 253-264
Storage: Store at 4 DEG C. -20 DEG C Long term AVOID FREQUENT FREEZE THAW CYCLES.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Catalog#: RDI-MBPabrt-12
Cost: $500.00/vial $438.00/vial 2 or more
clone (isotype) 12 (rat IgG)
Packaging: 2ml tissue culture supernatant and 0.1% sodium azide
Species: RAT
Myeloma/fusion partner: spleen cells from an immunized outbred rat were fused
with cells of the mouse NSO myeloma cell line
Source: supernatant
Purification Method: none
Antigen: Bovine myelin basic Protein
Specificity: Approx epitope between aa 82-87 (Martensen numbering)Reacts
weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is
broken. Synethetic peptide 82-99 reacts very well as does intact MBP
-Reacts with human, bovine, rabbit, guinea pig, rat ,mouse and sheep
MBP
-Cytochemical staining
-immunoblotting
-Elisa
Ref: -Groome, NP et al (1985) Neurochemistry Int 7 pp309-317
-Groome, NP et al (1988) J. Neuroimmunol 12 pp 253-264
-Gibson, B.W et al (1984) J. Biol. Chem 259:5028-5031.
Storage: Store at 4 DEG C. -20 DEG C Long term
AVOID FREQUENT FREEZE THAW CYCLES.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Catalog#: RDI-MBPabm-10
Cost: $500.00/vial $438.00/vial 2 or more
clone (isotype) 10 (mIgG2a)
Packaging: 2ml tissue culture supernatant with 0.09% sodium azide
Species: mouse
Myeloma/fusion partner: NSO myeloma cells fused with spleen cells from A/J
mice.
Source: TCS
Purification Method: none
Antigen: Bovine myelin basic Protein fragment (45-91) coupled to Ovalbumin
Specificity: Approx epitope between aa 82-91 (human) & 45- 91 (bovine)
(Martensen numbering) but not with the whole MBP molecule. The epitope is
located in the C terminal region of 82-91 and the corresponding epitope is
not present (or exposed) in whole MBP. Reacts wmost strongly with an unatural
sequence so this antibody is not suitable for RIA or ELISA. It can howvere
be used for immunoblotting of myelin degradation products and immunocytochemical
identification of MBP catabolsim products in the macrophages around the plaque
periphery in multiple sclerosis patients.
-Reacts with human and bovine
-Cytochemical staining MBP degradtion products -immunoblotting (degradation products)
Ref: -Groome, NP et al (1985) Neurochemistry Int 7 pp309-317
-Groome, NP et al (1988) J. Neuroimmunol 12 pp 253-264
Storage: Store at 4 DEG C. -20 DEG C Long term
AVOID FREQUENT FREEZE THAW CYCLES.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Catalog#: RDI-MBPabm-26
Cost: $500.00/vial $438.00/vial 2 or more
clone (isotype) 26 (mIgG1)
Packaging: 2ml tissue culture supernatant with 0.09% sodium azide
Species: mouse
Myeloma/fusion partner: SP2/0
Source: TCS
Purification Method: none
Antigen: Human myelin basic Protein
Specificity: Approx epitope between aa 67-74 (Martensen numbering) Sequence
Y G S L P Q
-Reacts with human
-Cytochemical staining
-immunoblotting
-Elisa
Ref: -Groome, NP et al (1985) Neurochemistry Int 7 pp309-317
-Groome, NP et al (1988) J. Neuroimmunol 19 pp 305-315
Storage: Store at 4 DEG C. -20 DEG C Long term
AVOID FREQUENT FREEZE THAW CYCLES.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Cost: $500.00/vial $438.00/vial 2 or more
clone (isotype) 1 (mIgG2a)
Packaging: 2ml Tissue culture supernatant with 0.2M Tris/Hcl pH 7.4 and 0.1%
sodium azide
Species: mouse
Myeloma/fusion partner: spleen cells from immunized NIH mice were fused with
cells of the NSO mouse myeloma cell line.
Source: tissue culture
Purification Method: none
Antigen: Bovine myelin basic Protein
Specificity: Approx epitope between aa 129-138 (Martensen numbering) Reacts
with MBP. the epitope retains its structure in Thermolysin digests.
-Reacts with human, bovine, and rat (weakly with rabbit)
-Cytochemical staining
-immunoblotting
-Elisa
Ref: -Groome, NP et al (1985) Neurochemistry Int 7 pp309-317
Storage: Store at 4 DEG C. -20 DEG C Long term
AVOID FREQUENT FREEZE THAW CYCLES.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Cost: $500.00/vial $438.00/vial 2 or more
clone (isotype) 14 (mRatIgG)
Packaging: 2ml tissue culture supernatant and 0.09% sodium azide
Species: RAT
Myeloma/fusion partner: somatic cell hybridization of NSO myeloma cells with
spleen cells from an outbred rat
Source: TCS
Purification Method: none
Antigen: Bovine myelin basic Protein
Specificity: Approx epitope between aa 36-50 (Martensen numbering) Recats
equally well with peptide and intact MBP. Sequence from FFGGDR to RFFGGO
region.
-Reacts with human, bovine, sheep and guinea pig (weakly with rabbit)
-Cytochemical staining
-immunoblotting
-Elisa
-immunoabsorbent chromatography
Ref: -Groome, NP et al (1985) Neurochemistry Int 7 pp309-317
-Groome, NP et al (1988) J. Neuroimmunol 12 pp 253-264
Storage: Store at 4 DEG C. -20 DEG C Long term
AVOID FREQUENT FREEZE THAW CYCLES.
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Myelin basic protein is a major component of the myelin sheath that coats
neurons of the central nervous system. The exact physiological function of
MBP remains unknown,although it has been implicated to play a role on the
maintenance of the structural integrity of myelin. MBP is phosphorylated
at five and possibly more sites in vivo.
In vitro, MBP is a substrate for phosphorylation by several different protein
kinases such as MAP kinase, cAmp dependent protein kinase, calmodulin dependent
kinase, protein kinase C and phosphorylase kinase. Even highly specific protein
kinases such as RAF1, MEK and Mekk can utilize MBP as an alternate substrate.
Catalog#: RDI-MYBP-10
Batch# see data sheet
Package Size: 10 milligrams $345.00 $315.00/package 2 or more
Supplied: lyophilized (from 10mM MOPS, pH 7.0)
Purity: 95% by SDS-Page analysis purified via CM-cellulose chromatography
MW: 18,400
Source: Bovine Brain
Storage: The material is stable for at least 2 years at 4 DEG C. Avoid frequent freeze thaw cycles after reconstitution.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Myelin Basic Protein (human)
CAT#: RDI-TRK8M79 CAT#: RDI-TRK8M79 $250.00/1mg $219.00/mg 2-9 $188.00/mg 10+
Source: Postmortal human brain
Blood sample from the tissue donor was tested and found negative for HBsAg,
HIV-1 and HIV-2 antibodies and HCV
Purity: More than 90% (5% SDS-PAGE).
Purification: Purified according to G.E. Diebler, Preparative Biochemistry
2(2) 139-165 (1972)
Immunological Activity: Immunoreactivity is checked using poly-and monoclonal
antibodies against MBP.
Presentation: lyophilized solid, essentially salt free (or liquid-see batch
sheet with shipment)
Store: -20 DEG C. Avoid repeated freeze-thaw cycles.
Protein Concentration: see actual batch data (determined by Lowry method
before lyophilization)
FOR IN VITRO RESEARCH USE ONLY
Myelin basic protein is a major component of the myelin sheath that coats neurons of the central nervous system. The exact physiological function of MBP remains unknown,although it has been implicated to play a role on the maintenance of the structural integrity of myelin. MBP is phosphorylated at five and possibly more sites in vivo.
In vitro, MBP is a substrate for phosphorylation by several different protein kinases such as MAP kinase, cAmp dependent protein kinase, calmodulin dependent kinase, protein kinase C and phosphorylase kinase. Even highly specific protein kinases such as RAF1, MEK and Mekk can utilize MBP as an alternate substrate.
Catalog#: RDI-MYBPDP-5
Batch# see data sheet
Package Size: 5 milligrams $344.00 $312.00/package 2+
Supplied: in 10mM MOPS, pH 7.0)
Derivation: purified from bovine brain and de- phosphorylated using lambda
protein phosphatase;no detectable endogeneous phosphorylation as determined
by immunoblotting 1ug of MBP with 1ug/ml of mouse anti-phos-MBP (cat#RDI-MBPDPabm
$531.00/100ug).
MW: 18,400
Source: Bovine Brain
Use: substrate for phosphorylation by several different protein kinases such
as MAPK, PKA, CamK, PKC, Raf1, MEK and MEKK.
Storage: The material is stable for at least 2 years at -20 DEG C. Avoid frequent freeze thaw cycles after reconstitution.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
see also human MBP and new dephosphorylated MBP
inquire for our monoclonals against myelin basic protein
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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