rev:  December 5, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Product: Goat anti-Macrophage Scavenger Receptor Antiserum

Cat No: RDI-MACROSRabG   $312.00/vial 0.25ml

Host: Goat

Clone: Polyclonal

Immunogen: Synthetic Peptide: 325 RPGNSGPKGQKGEKGSGN 342

                    (peptide available cat#RDI-MACROSR-PEP  $312.00/1mg)

Form: Whole serum

Concentration: N/A

Supplied as: 0.25ml vials

Titer: Reported working dilution using neat serum:*

- ELISA using immunizing peptide: 1:90K

-IHC using formalin-fixed, paraffin-embedded sections: 1:200

-IHC using paraformaldehyde-fixed, frozen sections: not tested

-Western blots: 1:2,000

Specificity: Amino acid sequence 325-342 of human macrophage scavenger receptor.

Preservative: 0.1% sodium azide (also available preservative free cat#RDI-MACROSCRabg-X  $1062.00/1ml)

Storage: Short term: Refrigerate at 4º C

              Long term: Freeze at -20º C

Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

FOR RESEARCH USE ONLY-Not for use In Diagnostics

Macrophage scavenger receptors (MSR) are membrane glycoproteins that mediate the recognition and uptake of a wide variety of negatively charged macromolecules. There are two types of these receptors that differ in the C-terminal region. MSR has a key role in scavenging a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid E-protein (AE). While the normal role of MSR is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and AE deposition in Alzheimer's disease (AD).

The observation that the ,4 allele of the apolipoprotein E gene is associated with an increased risk for AD has lead to the investigation of lipoprotein metabolism in the brain of AD patients. The type I MSR has been found to be expressed on microglia and Mato cells, but not astrocytes or neurons. In AD brain, MSR type I is strongly expressed by microglia cells that are associated with senile plaques. Because the senile plaques contain AGEs, which are a source of free radicals that could result in oxidative damage to the brain, MSR may have a role in mediating the endocytosis of AGEs.

A goat antiserum to a synthetic peptide that corresponds to amino acids 325-342 of the C-terminus of the human MSR is currently available. This antiserum has been shown to be immunoreactive with the unconjugated immunizing peptide by ELISA. The antiserum has been shown to be immunoreactive with amyloid plaques in formalin-fixed, paraffin-embedded sections of AD brain. This antiserum is suitable for western blots and recognizes the MSR protein. This antibody should be a valuable tool for scientists working to understand the role of MSR in AD.

This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide. Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification. A control immune serum (Cat no. RDI-GPA018E $315.00/500 µl) from a mock immunized animal is available to be used in conjunction with this antibody as well as the immunizing peptide which can be used to neutralize immunoreactivity.

Macrophage Scavenger Receptor C-terminal Peptide Antiserum

Alzheimer disease brain showing immunohistochemical staining of senile plaques at 1:200 dilution in formalin-fixed, paraffin-embedded section of hippocampus.

Western blot of Alzheimer’s disease  frontal cortex at 1:2,000 dilution.  The major MSR Type 1 band has a molecular weight of 75 kD,while minor bands have been reported to occur at ~55 kD and ~98 kD in AD brains.

Rat anti-mouse macrophage scavenger receptor

cat#  RDI-T2123X     $562.00

Monoclonal antibody 2F8 recognises the murine scavenger receptor types I and II. It inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. The molecule recognised is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues, e.g. atherosclerotic lesions.

Applications: immunohistology on frozen but not paraffin embedded sections, ELISA, Western Blotting, immunoprecipitation, FACS.

Lot number: 04PO0207

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2b

Quantity / Volume: 0.25mg / 0.25ml

Purity: Purified IgG from tissue culture supernatant

Physical state: liquid

Buffer: Phosphate buffered saline, pH 7.4

Preservative: 0.1% sodium azide

Stabilizer: none

Storage: Aliquots of stock solution can be kept frozen at -70°C;

Do not freeze working dilutions.

Stability of stock solution: 1 year at -70°C

Approximate working dilution: 1:50 - 1:100

Caution: this product contains sodium azide, a poisonous and hazardous substance

For laboratory use and research purposes only

REFERENCES:

Fraser I.P. et al.: Nature 364: 343-46 (1993)

de Villiers W.J.S. et al.: J. Exp. Med. 180: 705-709 (1994)

Hughes D.A. et al.: Eur. J. Immunol. 25: 466-73

Bell M.D. et al.: J. Neurocytol. 23: 605-613 (1994)

Hughes, D.A. et al.: Imm. Lett. 43: 7-14 (1994)

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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