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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


GAP-43 (Growth associated protein-43/neuromodulin)

Rabbit Polyclonal Antibody to GAP-43 (RAT)

Cat#RDI-PRO16024   $625.00

Category                       Rabbit polyclonal

Form/Purification          Rabbit serum

Immunogen                    Synthetic GAP-43 (221-226), conjugated to BSA

Description                   GAP-43, also called neuromodulin, B-50, pp46 and F1, is a neuron-specific, membrane-associated phosphoprotein involved in axonal growth and found in neurons undergoing regeneration.

Specificity                 The antiserum was raised against a synthetic C-terminal peptide of GAP-43.

                                   Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes staining,

Recommended Positive Control: Rat duodenum

Application · Immunofluorescence microscopy

                   · Suitable for frozen sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:400 - 1:800 using fluorescence, with overnight incubation at 2-8°C.

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

For Research Use Only


RABBIT ANTI-GROWTH ASSOCIATED PROTEIN 43 [GAP-43]

POLYCLONAL ANTIBODY

cat# RDI-GAP43abrX   $438.00

Presentation: 100ul diluted rabbit sera with 0.01% azide.

IMMUNOGEN: Purified GAP-43 from feline brain.

SPECIFICITY: Growth Associated Protein 43 [GAP-43].

Cross-Species: Human, monkey, mouse, rat, feline, bovine.

APPLICATIONS: -Immunocytochemistry: For mouse primary neurons,NTera2 neurons.

-Western blot: 1:500 1:1,000

-Immunoprecipitation

Optimal working dilutions must be determined by end user.

STORAGE: Store 4 DEG C (short term (1-2 weeks) or aliqout and store -20DEG C. Avoid frequent freeze thaw cycles. At first use, tigthen cap, centrifuge 1-2 minutes at 100rpm to concentrate material in vial.

REFERENCES: Visual Neurosci. (1990) 4:585-593.

                            Brain Research (1990) 518:324-328.

For research use only; not for use as a diagnostic.


Monoclonal anti-GAP43

cat# RDI-GAP43abm   $500.00/vial

Background: Growth associated phosphoprotein 43 (GAP43) is a major protein of neuronal growth cones and certain presynaptic terminals. It is a candidate for involvement in both axon growth and synaptic plasticity. It has been shown that in several neuronal systems, GAp43 expression is higher in neurons that are extending axons, either during development or regeneration of injured axons found in intact adult neurons. GAP43 is the best characterized of the growth associated proteins and although its normal action in vivo is unclear, GAP43 can bind calmodulin, inhibit phosphatidylinositol phsphate kinase,be phosphorylated by protein kinase C, affect neurotransmitter release and enhance filopodia in non-neuronal cells.


Specificity:* recognizes human GAP43

Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added.

Clone: 1G7

Isotype: mIgG2a

Antigen: Prokaryotic recombinant fusion protein corresponding to the full length GAP43 molecule

Hybridoma: mouse myeloma (p3-NS1-Ag4-1)

USES: -Paraffin sections

           -typical dilutions:1:20-1:40, 60 minutes   incubation at 25 DEG C with ABC detection

Stain: membrane and cytoplasmic

Controls: normal adult cerebral cortex

Reconstitution: 1 ml distilled water. Allow to set 30-60 minutes, mix well.

Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for immediate use. For long term use, recommend aliquoting and store at -20 DEG C. Avoid frequent freeze thaw cycles. Store in non frost free freezer.

general ref:

-Milosevic A et al, Immunocytochemical localization of growth-associated protein GAP43 in early human development. Development Brain research 84:282-288 (1995)

-Schuh S M et al, Production of the neuronal growth-associated protein GAP-43 in a bacterial expression system. Cancer Research 565:85-93 (1991)

-Strittmater S M , et al. Go is a major growth cone protein subject to regulation by GAP-43. Nature 344:836-841 (1990)

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


RDi Division of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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