rev:     July 2, 2004

HOME (index page)

Return (alphabetical antibody index page)

Return  (neurohistology ab index page)


ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


S100 (see below 6 monoclonals and polyclonals)  see also other S100 abs

-see also s100.pdf


ANTI-S100 PROTEIN MONOCLONAL ANTIBODY

PRODUCT CODE :RDI-CBL410      $438.00/vial    $375.00/vial 3 or more

CLONE: S1/61/69

ISOTYPE: IgG1

SOURCE: Tissue culture supernatant

PURIFICATION METHOD: None.

SPECIFICITY: S-100 can be found in glial cells and in tumours of the nervous system, such as schwannomas, ependydomas, and astrogliomas. The antibody also reacts with melanocytes and malignant melanoma, dendritic reticulum cells, adipocytes and chondrocytes. The antibody recognizes an antigenic determinant shared by both the a- and ß-subunit of bovine and human S-100.


APPLICATIONS:

* Useful as a marker in malignant melanoma.

* Reacts with paraffin wax embedded tissue sections.


PRESENTATION: 1.5ml Tissue culture supernatant, which should be diluted 1:10 to 1:100 depending on fixation and detection procedures to be used. The preparation is supplied containing 0.1% sodium azide as preservative.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Mouse anti-S100

cat# RDI-S100abm      $312.00/100ug

       RDI-S100abm-1   $438.00/200ug

Presentation: Protein A purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: purified bovine brain S100

Isotype: mIgG2a

Clone: 4C4.9

Reactivity: human, bovine, mouse

Uses: -Western blot: approx 0.5ug/ml

        -histochemistry (frozen and paraffin sections) approx 1:100-1:200 60 minutes RT

Positive Control melanoma or Schwannoma

Localization: Cytoplasmic

Background: Recognizes proteins of 21-24kDa, identified as the A & B subunits of S100 protein. S100 belongs to the family of   calcium binding proteins such as calmodulin and trop0onin C.   S100A is composed of an alpha and beta chain whereas S100B   is composed of two beta chains. Antibody to S100 stains Schwnnomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metasases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. The diagnosis of Histocytosis X is confirmed by S100 staining. S100 protein is highly soluble and may be eluted from frozen tissue during staining.

Storage: 2-8 deg c

For Research Use only


Rabbit anti-S100B

Catalog#: RDI-S100Babr

Price: $440.00/vial

Packaging: 200 microliters lyophilized antiserum in 15mM KPO4 ppH 8, approx 43mg/ml IgG, no preservatives)

Reconstitution: with 200ul distilled water

Species: rabbit

Antigen: purified bovine brain S100B

Specificity: specific for S100B polypeptide, showing <0.5% cross reactivity with S100A(alpha) and no cross reactivity with calmodulin. The antibody reacts with S100B from all species tested to date including bovine, rat, chicken, and human.

Uses: western blot: 1:200-1:1500

          Immunohistochemistry: 1:5000- 1:10K with avidin biotin method

         Elisa:

         RIA:1:1000

Optimal dilutions must be determined by the end user for their particular application.

ref: -Van Eldik L.J. and Griffin WST (1994), Biochim. Biophys Acta 1223:398-403

       -J. Neurochem 65:228-233

Storage: For long term storage, keep small portions (1-2ul) at -80 DEG C or at least -20 DEG C. For continuous use keep at 4 DEG C (with 0.01% Na-azide). AVOID FREQUENT FREEZE THAW CYCLES.

Allow 2 weeks for delivery:

Additional product of interest: recombinant human S100B cat#RDI-S100B-AG  $220.00/10ug

-For Research Use Only


Monoclonal mouse anti-human S-100 protein

Catalog # RDI-TRK4S37    $438.00/1mg   $375.00/mg 10+ or more Bulk Quotes on Request

Clone: clones 8B10, 6G1, 4B3, 3B10

These clones have been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with human S-100 protein.

Specificity: Clone 8B10, 6G10, 4B3 specific to S-100ab and S-100bb

                    Clone 3B10 - specific to S100aa

Mouse isotype: clones: 8B10, 6G1- IgG1, clones 4B3, 3B10 - IgG2a.

Application: S-100 immunoassay and immunoaffinity purification. All clones are working in Western blotting.

Recommended pairs for sandwich immunoassay: (coating - conjugate) 8B10 - 6G1, 3B10 - 6G1,

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7.4 containing 0,1% of sodium azide as preservative. Store at +4C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.


Human S-100 Antigen:   RDI-S100AG              Human S-100 Protein                    

100ug  $406.00, 500ug=$750.00  1mg              $1250.00

see other S100 antigen from human ag table


ANTIBODIES AGAINST S-100


cat#RDI-S100abg            Goat anti-S100   $312.00/1ml

-made against bovine brain S-100 purified by SDS-Page

-suitable for immunohistochemistry and EIA

-sold as 1ml neat serum

-no other data available


cat#RDI-S100abs           Sheep anti-S100    $312.00/1ml

-made against bovine brain S-100 purified by SDS-Page

-suitable for immunohistochemistry and EIA

-sold as 1ml neat serum

-no other data available


cat#RDI-S100Labm     mouse anti-human S100L

$438.00/50ug   $750.00/150ug

-made against bovine S100L

-mIgG1 packaged at 250ug/ml in 50% glycerol:PBS

-reacts with human S100L in immunofluorescence and in  western blot (10kda using MDBK cells)


cat#RDI-S100Pabm     mouse anti-human S100P

$438.00/50ug  $750.00/150ug

-made against human S100P

-mIgG1 packaged at 250ug/ml in 50% glycerol:PBS

-reacts with human S100P in western blot (11kda using human  placenta cells)

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-NOT FOR DIAGNOSTICS.

see also antibodies to calmodulin and related products-please inquire.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

Ordering terms

RETURN(neurohistology ab page)