rev:  October 4, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CD56 ANTIGEN (NEURAL CELL ADHESION MOLECULE)

     see also our CD table for additional antibodies against CD56


Mouse anti-human CD56 (NCAM) Paraffin Sections

cat#RDI-CD56abm-C3    $406.00/200ug vial

-mouse monoclonal IgG1

-human NCAM (CD56) reactive

-suitable for western blotting, immunoprecipitation and immunohistochemistry (formalin-fixed paraffin embedded sections with high temp release, certain tissues may requrie trypsin or 0.1% saponin pretreatment)

-also available in kit form sufficient for 150 slides, cat#RDI-CD56abm-PKT   $625.00



ANTI-Human CD56 ANTIGEN   (NEURAL CELL ADHESION MOLECULE)

PRODUCT CODE :RDI-CBL159    $375.00/200ug vial

CLONE: ERIC-1

ISOTYPE: IgG1

Immunizing Antigen: human retinoblastoma tissue

Fusion Partner: P3X6Ag8.653 myeloma cell line

SOURCE: tissue culture supernatant

PURIFICATION METHOD: Protein A affinity chromatography

SPECIFICITY: The CD56 antogen is a 175-185kDa isoform of the neural cell adhesion molecule (NCAM). It is expressed by lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.

APPLICATIONS: * Recognition of a membrane antigen present on retinoblastoma and other neuroectodermal tumours and tissues.

                             * Immunohistological characterisation of neuroectodermal tumours and in detailed biochemical         investigation of expression of different isoforms of NCAM’s on different tumours.

-This isoform works on frozen sections but NOT in flow cytometry

-Identification of NK cells

-immunoprecipitation studies of CD56 antigen

-Also reacts with porcine antigens

REFERENCES: Bourne, S. et al. J.Neuro-oncology 10, 111-119 (1991)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -200C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in bulk form (no BSA, NO AZIDE),

order cat#RDI-CBL159-1XP $1062.00/1 milligram Significant discounts for bulk-please inquire.


Mouse Anti-Human CD56


PRODUCT CODE : RDI-CBL510     $375.00/vial 200ug

also available FITC labeled cat#RDI-CBL510FT    $375.00/100 Test vial

                         PE labeled cat#RDI-CBL510PE    $469.00/100 Test vial



CLONE: Mem 188


ISOTYPE: IgG2a


SOURCE: mouse ascitic fluid


Fusion Partner: NSO myeloma cell line


PURIFICATION METHOD: ion exchange chromatography



SPECIFICITY: CD56 is an isoform of NCAM molecule that is expressed by all lymphocytes that mediate non- MHC restricted cytotoxicity. Mem 188 is specific for the extracellular domain of NCAM that consists of five C2 set IgSF domains and 2 fibronectin III domains.


APPLICATIONS:

*FACS analysis of PBLs and other CD56+ cells

* Antibody is positive in non reduced western blotting

* immunoprecipitation of 125I labeled CD56+ cells

*Pan NK cell marker


Cross Reactivity: also known to be reactive with rhesus monkey lymphocytes


References:

Lanier, L.L. et al (1991) J. Immunol. 146 4421-26

Edelman, G.M. & Crossin K.L. (1991) ANN Rev. Biochem 60:155-190


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME order cat#RDI-CBL498-1XCP $1062.00/1mg



Antibodies to N-CAM (CD56)

Expression of neural cell adhesion molecules (N-CAM) provides neurons with a means of attaching to and interacting with other cells and the extracellular matrix. Alternative splicing of N-CAM mRNA results in several N-CAM isoforms. The three major isoforms are referred to as 180 kD, 140 kD, and 120 kD. Individual isoforms undergo various post-translational modifications, including the addition of polysialic acid residues to embryonic forms. The highly polysialylated forms of N-CAM present in embryos and neonates are lost during maturation and replaced by less-polysialic  acid-laden mature forms of N-CAM. The functional significance of  alternative forms of N-CAM remain to be fully elucidated. RDI offers N-CAM antibodies recognizing the major isoforms of adult and embryonic N-CAM (N-CAM 13), and embryonic N-CAM (12F8).


Adult and Embryonic Pan N-CAM (mouse/rat)

cat #RDI-NCAM13abm      $469.00/vial 100ug

Clone: N-CAM13

Isotype: Mouse IgG2a

Immunogen: Mouse brain membrane fractions.

Format: Purified

Size: 0.1mg

-N-CAM 13 recognized the three major isoforms of N-CAM, referred to as 120 kD, 140 kD, and 180 kD. It recognized an extracellular epitope. N-CAM 13 has been shown to react with mouse and rat. It does not cross-react with chicken. Applications include western blot analysis, immunohistochemistry of frozen sections and of tissue-cultured neurons.


Rat anti-N CAM (Adult and Embryonic N-CAM (140 and 180 kD Epitopes)

cat# RDI-CD56-12F11ab    $469.00/vial

Clone: 12F11

Isotype: Rat IgG2a

Format: Purified

Size: 0.1 mg

Clone 12F11 recognizes the 140 and 180 kD isoforms of N-CAM, but not the 120 kD form. 12F11 recognizes an intra-cellular epitope. 12F11 has been shown to react with human,rat,mouse, and chick  neuronal N-CAM. Applications include western blot analysis, and  immunohistochemistry of frozen tissue sections for light and electron microscopy, and of tissue-cultured neurons. Immunogen: Mouse brain membrane fractions.

1. Chung,W.W., C. F. Lagenaur,Y. Yan, and J.S. Lund. 1991. Developmental expression of neural cell adhesion molecules in the mouse neocortex and olfactory bulb. J.Comp.Neurol. 314:290-305.

2. Miller, P.D.,W.W. Chung,C.F. Lagenaur, and S.T. DeKosky. 1993. Regional distribution of neural cell adhesion molecule (N-CAM) and L1 in human and rodent hippocampus. J. Comp. Neurol. 327:341-349.

3. Miller, P.D.S.D. Styren, C.F. Lagenaur, and S.T. DeKosky,1994. Embryonic neural cell adhesion molecule (N-CAM) is elevated in thedenervated rat dentate gyrus. J. Neurosci. 14: 4217-4225.

4. Jankin, M.H. and C. F. Lagenaur. 1994. Cell adhesion molecules in the early developing mouse retine: Retinal neurons show preferential outgrowth in vitro on L1 but not N-CAM. J. Neurobio. 25: 472-487.


Rat anti-Embryonic N-CAM

cat #RDI-CD56-12F8     $469.00/100ug vial

Clone: 12F8

Isotipe: Rat IgM

Format: Purified

Size: 0.1 mg

Clone 12F8 reacts with the polysialic acid on N-CAM. The epitope recognized is a neuraminidase-sensitive carbohydrate. Additionally, it may cross-react with carbohydrate epitopes that may be expressed in the embryonic than in the adult brain. As such, 12F8 is useful for distinguishing embryonic forms of N-CAM from adult forms which have decreased polysialic acid expression. 12F8 will also react with some non-neuronal cells expressing polysialylated N-CAM, however, it does not appear to recognize mouse NK cells. 12F8 recognized all species tested, from fish through human. Published references include human, rat, mouse, and avian. Applications include western blot analysis, and immunohistochemistry of  frozen tissue sections.

Immunogen: Mouse brain membrane fractions.

1. Chung,W.E., C.F. Lagenaur,Y.Yan, and J.S. Lund. 1991. Developmental expression of neural cell adhesion molecules in the mouse neocortex and olfactory bulb. J.Comp.Neurol. 314: 290-305.

2. Miller, P.D., W.W. Chung,C.F. Lagenaur, and S.T. DeKosky, 1993. Regional distribution of neural cell adhesion molecule (N-CAM) and L1 in human and rodent hippocampus. J.Comp.Neurol. 327:341-349.

3. Miller, P.D., S.D. Styren,C.F. Lagenaur, and S.T. DeKosky. 1994. Embryonic neural cell adhesion molecule N-CAM) is elevated in the denervated rat dentate gyrus. K/Meurosci. 14:4217-4225. Hankin,M.H., and C.F. Lagenaur. 1994. Cell adhesion molecules in the early developing mouse retine: Retinal neurons show preferential outgrowth in vitro on L1 but not N-CAM. J. Neurobio. 25: 472-487.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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