rev: October 4, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse ANTI-Human CD44 ANTIGEN (HCAM)

-see also our CD table for additional monoclonal and polyclonals against CD44



PRODUCT CODE :RDI-CBL154    $375.00/vial 200ug purified

      FITC label 100 tests $375.00 ord cat#RDI-CBL154FT

         PE label 100 tests $469.00 ord cat#RDI-CBL154PE


CLONE: F10-44-2


ISOTYPE: IgG2a


SOURCE: Mouse ascitic fluid


PURIFICATION METHOD: Ammonium sulphate precipitation followed by ion exchange chromatography


SPECIFICITY: The mature CD44 antigen is a transmembranous molecule which has undergone both extensive O-linked and N-linked glycosylation. The main cellular reactivity is on leucocytes and red blood cells with only weak reactivity on platelets.


APPLICATIONS:

* Identification of CD44 - positive cells in immunocytochemistry and flow cytometry.

* Studies involving T -, B - cell, monocyte and granulocyte binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

* Studies on HCAM (homing receptor) function.


REFERENCES:

Dalchau, R. in Leucocyte Typing III. Oxford University Press 1987.

Dalchau, R. in Leucocyte Typing IV. Oxford University Press 1989.

Goldstein, L.A. & Butcher, E.C. Immunogenetics 32 (6) 389-97 (1990).


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

FOR BULK (NO BSA AND NO AZIDE): $1062.00/1 milligram


-BULK FITC (milligram or larger quantities quoted on request).



RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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