rev: July 15, 1999

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ANTIBODIES  

                                                                  (anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Cat. No. RDI-ASYNUCabG   $315.00/0.25ml


-A goat antiserum to a synthetic peptide that corresponds to amino acids 116 - 131 of the C-terminus of the Parkinson disease Alpha-synuclein protein is currently available. The Alpha-synuclein gene was discovered on chromosome 4 and is responsible for early-onset autosomal dominant Parkinson’s disease. It is an abundant protein whose major site of expression is in the nervous system. Human ALPHA-synuclein is also known to be a precursor of the non- -amyloid component protein (NACP) of Alzheimer’s disease where one of its fragments associates amyloid deposits.

-The function of this gene product is currently unknown, but it is being shown to be associated with other neurodegenerative diseases, such as dementia with Lewy bodies and multiple-system atrophy. This antiserum has been shown to be immunoreactive to the ALPHA-synuclein immunizing peptide by ELISA. Currently, this antibody has been shown to immunolabel Lewy bodies in Parkinson brains using formalin-fixed, paraffin-embedded sections. This antibody should be a valuable tool for scientists who will be working to discover the function of this protein in normal individuals and its role in Parkinson’s and other neurodegenerative diseases.

-References:

-Spillantini M. G., et al. A-synuclein in Lewy bodies. Nature 388: 839-840, 1997.

-Gai, W. P., et al. Multiple-system atrophy: a new A-synuclein disease. Lancet 352: 547-548, 1998.

 DESCRIPTION: ALPHA -Synuclein C-terminal Peptide Antiserum

PRODUCT NO: RDI-ASYNUCabG $312.00/0.25ml

LOT NO:

ANTIBODY HOST: Goat

IMMUNOGEN: Synthetic peptide corresponding to amino acids 116-131 of the C-terminus of the human ALPHA -Synuclein protein. 

Antigen sequence: M P V D P D N E A Y E M P S E E  (cat#RDI-ASYNUC-CPX $469.00/1mg)

FORM: Whole antiserum

CONCENTRATION: N/A

TITER: Recommended dilutions based of neat serum:
-   ELISA using immunizing peptide: approx 1:1,000
-  IHC formalin-fixed, paraffin sections: approx 1:300
-  IHC paraformaldehyde-fixed, frozen sections: n/d
-Western Blots: n/d

SPECIFICITY:Specific for residues 116-131 on the C-terminus of human ALPHA-Synuclein Protein 

STORAGE: 4° C short term; -20° long term

FOR RESEARCH AND MANUFACTURING USE ONLY.

Goat ALPHA-Synuclein Antiserum

 Parkinson disease brain showing immunohistochemical staining of Lewy body at 1:300 dilution in formalin-fixed, paraffin-embedded section of substantia nigra.

 RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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