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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since
no one antibody works best for all applications (neutralization, blotting,
histochemistry, ELISA, etc), we offer many different types of antibodies
to help solve this problem. Please inquire for other applications or types
of antibodies not listed below.
Product: Rabbit
anti- Amyloid Beta N-terminal Antiserum
Cat
No:
RDI-AMYLBNabR
$375.00/0.25ml
Host: Rabbit
Clone: Polyclonal
Immunogen:
Synthetic Peptide: 1 DAEFRHDSGYEVHH
14
Form:
Whole serum with 0.1% sodium azide
Azide free (order
cat#RDI-AMYLBNabG-XP
$1250.00/1ml)
Concentration:
N/A
Supplied
as:
0.25 ml vials
Titer:
Reported working dilution using neat serum:*
-ELISA using immunizing peptide:
approx 1:1,000
-IHC using formalin-fixed, paraffin-embedded sections: approx
1:200
-IHC using paraformaldehyde-fixed,
frozen sections: N/D
-
Western blots:
N/D
Specificity:
Amino acid sequence 1-14 of human amyloid Beta
protein.
Storage:
Short term: Refrigerate at
4º
C (1-2 months)
Long term: Freeze at
-20º
C. (recommend aliquot and store
–20 DEG C.
Avoid
frequent freeze thaw cycles)
FOR
RESEARCH AND MANUFACTURING USE ONLY
*
Various assay conditions require that the optimum working concentrations
be determined by serial dilutions of this product.
Product:
Human Amyloid Beta N-terminal Peptide Antiserum
Cat
No: RDI-AMYLBNabR
Amyloid Beta (ABeta)
is the peptide that accumulates in the brain in Alzheimer's disease and certain
classes of cerebrovascular angiopathy. Alzheimer’s disease (AD) is
characterized by the progressive formation in the brain of insoluble amyloid
plaques and vascular deposits consisting of the 4-kD
ABeta-peptide. ABeta generation
is initiated by proteolytic cleavage of the amyloid precursor protein (APP)
at the N-terminal of ABeta by
Beta-secretase. The ABeta peptide
is then released by proteolytic cleavage at its C-terminus by
(-secretase.
The insoluble ABeta peptide forms aggregates in the vessels and senile
plaques of AD brains.
A rabbit antiserum to
a synthetic peptide that corresponds to amino acids 1-14 of the Nterminal
sequence of Abeta has been produced and is currently
available. This antiserum has
been shown to be immunoreactive with the unconjugated immunizing peptide
by ELISA. This antiserum is
capable of detecting ABeta by immunohistochemistry or protein immunoblots
and will identify it from other CNS amyloids. Using formic acid treatment
of formalin-fixed, paraffin-embedded sections, this antiserum will detect
both cerebrovascular and core Abeta in senile plaques. It will also detect
ABeta on immunoblots of formic acid extracts of brain tissue. The
immunohistochemical detection of ABeta has been shown to be more sensitive
than histochemical methods. This
antibody should be a valuable tool for scientists working to understand the
role of ABeta in Alzheimer’s disease.
This antiserum was produced
using proprietary methodology whereby the peptide is attached to a carrier
that elicits minimal immunoreactivity so that the antiserum has a higher
degree of specificity for the peptide.
Since there is no overwhelming production of interfering antibodies
to the carrier, this antiserum can routinely be used without further
purification. A control immunizing
peptide (RDI-AMYLBN-CPX
$312.00/1mg) is available to be used in conjunction with this antibody
to neutralize immunoreactivity.
Amyloid
Beta N-terminal Peptide Antiserum
Cat No. RDI-AMYLBNabR
Alzheimer
disease brain showing
immunohistochemical
staining of vessels containing amyloid Beta deposits at
1:200 dilution in formalin-fixed, paraffin-embedded,
formic-acid-treated section
of
hippocampus.
Alzheimer disease brain
showing
immunohistochemical
staining of amyloid cores in senile plaques containing amyloid
Beta deposits at 1:200 dilution in formalin-fixed,
paraffin-embedded,
formic-acid-treated section of hippocampus.
For Research Use Only
See other antibodies
at:
http://www.researchd.com/absort.htm
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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