rev:March 19,2001

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD70 Antibodies

DATA SHEET: Rat anti mouse CD70

Catalog#: RDI-MCD70abrt   Price: $281.00/1pkg

  also available:Biotin labeled                cat#RDI-MCD70ab-BT $594.00/0.5mg

                         PE labeled                     cat#RDI-MCD70ab-PE $625.00/01.mg

                 no azide/sterile filtered         cat#RDI-MCD70ab-XP $625.00/0.5mg

Package Size: 0.1mg in 0.2 10mM Phosphate buffer, pH7.2 with   150mM NaCl and 0.1% sodium azide.


Supplied: purified by affinity chromatography

Clone: FR70

Immunogen: BaLB/c mouse B lymphoma A20.2J

Ig Isotype: rat IgG2bk

Uses: -flow cytometry (<1ug/1M cells),

          -function blocking studies (azide free)

          -histochemistry(acetone fixed frozen sections)

         -immunoprecipitation

Background: The FR70 antibody reacts with mouse CD70, 1 a 30-33 kDa type-II transmembrane glycoprotein belonging to the TNF/NGF superfamily.2 CD70 has been identified as the counter-receptor for CD27 and is expressed primarily on activated B cells and at very low levels on some activated T cells. CD70 expression can be induced by activation of splenocytes with Lipopolysaccharide or anti-mouse CD40 mAb HM40-3 or clone 3/23 , but not Concanavalin A. The CD27-CD70 interaction delivers a T-cell costimulatory signal. It has been reported that FR70 mAb blocks binding of mCD27-Ig fusion protein to mCd70-transfected cells and inhibits some in vitro T-cell dependent responses.

ref: 1. Oshima, H., H. Nakano, C. Nohara, T. Kobata, A. Nakajima, N.A. Jenkins, D.J. Gilbert, N.G. Copeland, T. Muto, H. Yagita, and K. Okumura. 1998. Characterization of murine CD70 by molecular cloning and mAb. Int. Immunol. 10: 517 - 526.

2. Tesselaar, K., L.A. Gravestein, G.M.W. van Schijndel, J. Borst, and R.A.W. van Lier. 1997. Characterization of murine CD70, the ligand of the TNF receptor family member CD27. J. Immunol. 159: 4959 - 4965.

3. Zola, H. 1995. Detection of cytokine receptors by flow cytometry. In Current Protocols in Immunology. Coligan, J.E., A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, NY, pp. 6.21.1 - 6.21.18.

Storage: Store at 4 Deg C.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com