rev: October 3, 2001

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD56 (NCAM-1) Antibodies

Antibodies to N-CAM (CD56)

Expression of neural cell adhesion molecules (N-CAM) provides neurons with a means of attaching to and interacting with other cells and the extracellular matrix. Alternative splicing of N-CAM mRNA results in several N-CAM isoforms. The three major isoforms are referred to as 180 kD, 140 kD, and 120 kD. Individual isoforms undergo various post-translational modifications, including the addition of polysialic acid residues to embryonic forms. The highly polysialylated forms of N-CAM present in embryos and neonates are lost during maturation and replaced by less-polysialic  acid-laden mature forms of N-CAM. The functional significance of  alternative forms of N-CAM remain to be fully elucidated. RDI offers N-CAM antibodies recognizing the major isoforms of adult and embryonic N-CAM (N-CAM 13), and embryonic N-CAM (12F8).

Adult and Embryonic Pan N-CAM (mouse/rat)

cat #RDI-NCAM13abm      $469.00/vial 100ug

Clone: N-CAM13

Isotype: Mouse IgG2a

Immunogen: Mouse brain membrane fractions.

Format: Purified

Size: 0.1mg

-N-CAM 13 recognized the three major isoforms of N-CAM, referred to as 120 kD, 140 kD, and 180 kD. It recognized an extracellular epitope. N-CAM 13 has been shown to react with mouse and rat. It does not cross-react with chicken. Applications include western blot analysis, immunohistochemistry of frozen sections and of tissue-cultured neurons.

Rat anti-N CAM (Adult and Embryonic N-CAM (140 and 180 kD Epitopes)

cat# RDI-CD56-12F11ab    $469.00/vial

Clone: 12F11

Isotype: Rat IgG2a

Format: Purified

Size: 0.1 mg

Clone 12F11 recognizes the 140 and 180 kD isoforms of N-CAM, but not the 120 kD form. 12F11 recognizes an intra-cellular epitope. 12F11 has been shown to react with human,rat,mouse, and chick  neuronal N-CAM. Applications include western blot analysis, and  immunohistochemistry of frozen tissue sections for light and electron microscopy, and of tissue-cultured neurons. Immunogen: Mouse brain membrane fractions.

1. Chung,W.W., C. F. Lagenaur,Y. Yan, and J.S. Lund. 1991. Developmental expression of neural cell adhesion molecules in the mouse neocortex and olfactory bulb. J.Comp.Neurol. 314:290-305.

2. Miller, P.D.,W.W. Chung,C.F. Lagenaur, and S.T. DeKosky. 1993. Regional distribution of neural cell adhesion molecule (N-CAM) and L1 in human and rodent hippocampus. J. Comp. Neurol. 327:341-349.

3. Miller, P.D.S.D. Styren, C.F. Lagenaur, and S.T. DeKosky,1994. Embryonic neural cell adhesion molecule (N-CAM) is elevated in thedenervated rat dentate gyrus. J. Neurosci. 14: 4217-4225.

4. Jankin, M.H. and C. F. Lagenaur. 1994. Cell adhesion molecules in the early developing mouse retine: Retinal neurons show preferential outgrowth in vitro on L1 but not N-CAM. J. Neurobio. 25: 472-487.

Rat anti-Embryonic N-CAM

cat #RDI-CD56-12F8     $469.00/100ug vial

Clone: 12F8

Isotipe: Rat IgM

Format: Purified

Size: 0.1 mg

Clone 12F8 reacts with the polysialic acid on N-CAM. The epitope recognized is a neuraminidase-sensitive carbohydrate. Additionally, it may cross-react with carbohydrate epitopes that may be expressed in the embryonic than in the adult brain. As such, 12F8 is useful for distinguishing embryonic forms of N-CAM from adult forms which have decreased polysialic acid expression. 12F8 will also react with some non-neuronal cells expressing polysialylated N-CAM, however, it does not appear to recognize mouse NK cells. 12F8 recognized all species tested, from fish through human. Published references include human, rat, mouse, and avian. Applications include western blot analysis, and immunohistochemistry of  frozen tissue sections.

Immunogen: Mouse brain membrane fractions.

1. Chung,W.E., C.F. Lagenaur,Y.Yan, and J.S. Lund. 1991. Developmental expression of neural cell adhesion molecules in the mouse neocortex and olfactory bulb. J.Comp.Neurol. 314: 290-305.

2. Miller, P.D., W.W. Chung,C.F. Lagenaur, and S.T. DeKosky, 1993. Regional distribution of neural cell adhesion molecule (N-CAM) and L1 in human and rodent hippocampus. J.Comp.Neurol. 327:341-349.

3. Miller, P.D., S.D. Styren,C.F. Lagenaur, and S.T. DeKosky. 1994. Embryonic neural cell adhesion molecule N-CAM) is elevated in the denervated rat dentate gyrus. K/Meurosci. 14:4217-4225. Hankin,M.H., and C.F. Lagenaur. 1994. Cell adhesion molecules in the early developing mouse retine: Retinal neurons show preferential outgrowth in vitro on L1 but not N-CAM. J. Neurobio. 25: 472-487.

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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com