Mouse anti-Na+/K+ alpha 1 subunit Monoclonal ab (M7PBE9)

cat# RDI-atpa1M7abm $469.00/vial

Background: The NA+/K+ Atpase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of NA and K across the cell membrane. The enzyme consists of a large multipass transmembrane catalytic subunit (alpha subunit) and an associated smaller glycoprotein (Beta subunit). Studies indicate that there are three isoforms of the alpha subunit (A1, a2, & A3) and two isoforms of the Beta subunit (B1 & B2) which are encoded by two multigene families. The alpha 3 subunit has been found in neuronal and to a lesser extent skeletal and smooth muscle.

isotype: mIgG1

clone: M7-PB-E9

package: 200ul of diluted ascites fluid with 0.05% NaN3

antigen: produced from a fusion of SP20/0-Ag14 myeloma cells and spleen cells from F1 hybrid mice immunized with purified SHEEP kidney alpha subunit Na+ K+ Atpase.

Specificity: alpha 1 subunit of NA+/K+ ATPase -in human, sheep, canine , chicken and pig. (also sees alpha 3 subunit in rat but not alpha 1)

-binds to region between amino acids 646 and 652 of the sheep kidney NA K Atpase alpha subunit.

Use: -western blot (detects a band at approx 110kDa) 1:250

-immunoprecipitation

-Immunohistochemistry: 1:100

ref: -W.J. Ball, et al, Biochimica et Biophysica Acta, 719:413-423, 1982.

-A. Abbott and W.J. Ball, Biochemistry, 32:3511-3519, 1993

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.