rev:  September 24, 2002

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


Anti-MOUSE  CD54 (ICAM-1) Antibodies

-2 clones


DATA SHEET: Hamster anti mouse ICAM-1 (CD54), monoclonal

Catalog#: RDI-MCD54-3E2   Price: $469.00/1pkg    BULK ON REQUEST

Package Size: 0.5mg in 1.0ml

Supplied: purified ab in Tris-Hcl with 0.1%NaN3

Clone: 3E2

Ig Isotype: armenian Hamster IgG

Purification: Purified from ascites

Reactivity: Reacts with ICAM-1, a 95 kDa surface antigen found on endothelial cells, lymphocytes, epithelial cells, dendritic cells, and keratinocytes. Icam-1 is a ligand for LFA-1 (CD11a/CD18) and MAC-1(CD11b/CD18). Its expression is up regulated upon stimulation by inflammatory mediators such as cytokines and LPS and its involved in B cell T-cell co- stimulatory interactions.

USE: flow cytometry(<1ug/1M cells)

        histochemistry (acetone fixed frozen sections)

        immunoprecipitation

        blocking (azide free material)

ref: -J. Immunol. 150:655-663

       -J. Exp. Med. 180:95-109

Storage: Store at 4 Deg C. Aliquot. Avoid   frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

also available: FITC labeled cat#RDI-MCD54-FT $625.00/0.5mg

no azide formulation cat#RDI-MCD54-XP $625.00/0.5mg

Biotin conjugates upon request


RAT MONOCLONAL ANTIBODY TO: MOUSE CD 54 (MOUSE ICAM-1)

PRODUCT CODE                         RDI-CBL1331       $400.00

CLONE                                          LTF 653

ISOTYPE                                       Rat IgG2b

SOURCE                                        Mouse ascitic fluid

PURIFICATION METHOD           Protein G affinity chromatography

SPECIFICITY                                This antibody recognizes mouse intercellular adhesion molecule-1 (ICAM-1). ICAM-1 is a 90-110KDa transmembrane glycoprotien expressed by monocytes, lymphocytes, dendritic cells and endothelial cells. In the inflammatory response interaction of CD54 with its ligands CD11a and CD 11b is important. Expression of CD54 is upregulated on activated lymphocytes.

APPLICATIONS                          Blocking of CD54 in functional studies.*Flow cytometry                                                                                                       analysis*Immunohistochemistry on frozen sections

LOT SPECIFIC DATA

PRESENTATION    The monoclonal is presented at 0.5mg in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available:

FITC conjugated cat#RDI-CBL1331FT $375.00/1000T

PE conjugated cat#RDI-CBL1331PE $438.00/100T

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


RAT MONOCLONAL ANTIBODY TO: MOUSE CD 54 (MOUSE ICAM-1)

PRODUCT CODE                            RDI-CBL1316      $375.00

CLONE                                             KAT-1

ISOTYPE                                          Rat IgG2a

IMMUNIZING ANTIGEN                Mouse mastocytoma p815 cells

FUSION PARTNER                         Spleen cells of rat fused with the mouse myeloma cell line P3-X63 Ag8.653

SOURCE                                          Mouse ascitic fluid

PURIFICATION METHOD              Protein G affinity chromatography

SPECIFICITY                                   This antibody recognizes mouse intercellular adhesion molecule-1 (ICAM-1). It also inhibits the cytotoxic activity of LAK cells through its binding with cell surface antigen of target cells. It inhibits homotypic cell aggregation of PMA (TPA) - stimulated PHA blasts and EL-4 cells.

APPLICATIONS                            *Flow cytometry analysis*Immunohistochemistry on frozen sections

PRESENTATION The monoclonal is presented at a concentration of 0.1mg at 1mg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


-All products are for in vitro research use only-

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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com