rev: April 18, 2003
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CD CLUSTERED ANTIBODIES
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
cat# RDI-MCD53-OX79 $531.00
Presentation: 0.5mg in 1ml (0.5mg/ml) buffer with 0.09% sodium azide
Immunogen: BAB/14 mouse macrophage cell line RAW 264 1
PREPARATION: purified from tissue culture supernatant by affinity chromatography.
Storage: undiluted at 4°C. Avoid frequent warming cycles. DO Not Freeze
USE: -indirect FACS (approx 1ug/million cells)
-western blot analysis 1-2ug/ml
-histochemistry of acetone-fixed frozen sections
(not recommended for paraffin sections) 5-10ug/ml
Specificity: The OX-79 antibody reacts with CD53, 1 a 35-45 kDa member of the Transmembrane 4-pass protein superfamily (TM4SF).2 As in the human, mouse peripheral leukocytes express CD53 mRNA and protein, and erythrocytes and non-hematopoietic cells do not. However, the distribution of CD53 in the mouse and rat thymus differs from that in the human.1 In the mouse, most CD4 + 8 - and major subsets of CD4 - CD8 - (double-negative) and CD4 - CD8 + thymocytes express CD53, while most cortical CD4 + CD8 + (double-positive) thymocytes are CD53-low or CD53-negative. CD53 expression can be induced in double-positive thymocytes by cross-linking of their T-cell receptors with anti-TCR mAb or peptide-pulsed antigen-presenting cells. There is a strong correlation between positive selection of thymocytes and CD53 expression. Its association with CD2 and a tyrosine phosphatase in rat T lymphocytes 3 and with CD19, CD21, HLA-DR, and other TM4SF proteins in human B lymphocytes 4 suggests that CD53 is involved in leukocyte signal transduction.
REFERENCES: 1. Tomlinson, M.G., T. Hanke, D.A. Hughes, A.N. Barclay, E. Scholl, T. Hunig, and M.D. Wright. 1995. Characterization of mouse CD53: epitope mapping, cellular distribution and induction by T cell receptor engagement during repertoire selection. Eur. J. Immunol. 25: 2201 - 2205.
2. Puls, K.L., K.A. Hogquist, N. Reilly, and M.D. Wright. 2002. CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. Int. Immunol. 14: 249 - 258.
3. Carmo, A.M., and M.D. Wright. 1995. Association of the transmembrane 4 superfamily molecule CD53 with a tyrosine phosphatase activity. Eur. J. Immunol. 25: 2090 - 2095.
4. Angelisova, P., I. Hilgert, and V. Horejsi. 1994. Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 39: 249 - 256.
For In Vitro research use-not for use in or on humans or animals. Not responsible for patent infringements with use or derivation of any product.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds
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