rev: October 3, 2001

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD5  Antibodies

DATA SHEET: Rat anti-Mouse CD5 LY-1)

Catalog#: RDI-RamCD5-53   $438.00/vial

Package Size: 500ug purified ab with 0.1% NaN3

Species: rat IG2ak

CLONE: 53-7.3

Activity: Reacts with mouse CD5 (LY-1), the ligand of CD72, found on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on NK cells. Phenotypic, anatomical, functional, developmental, and pathogenic characteristics of the CD5+ B cells   suggest that they may represent a distinct lineage, known as B-1 cells. The frequency of CD5+ B cells shows strain-dependent variation. The level of CD5 antigen detected on T helper cells is somewhat higher than that on T cytotoxic/supressor and B cells. Few if any intestinal intraepithelial lymphocytes bearing the gamma-delta T cell receptor express CD5. This clone recognizes an epitope of CD5 which is expressed on all strains tested. Phenotypic, anatomical, functional, developmental and pathogenic characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. The frequency of CD5+ B cells shows strain dependent variation. It has been proposed that CD5 negatively regulates signal transduction mediated by T-cell receptor and B cell receptor.

Uses: paraffin embedded sections (recommend zinc fixed paraffin sections, not formalin fixed)

         -Acetone fixed frozen sections

          -immunoprecipitation

          -immunofluorescent detection (recommend PE or PerCp conjugates due to low levels)

ref: Immunol Rev. 47:63-90.

      J. Immunol 127:2594-2604

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Also available conjugated to Biotin: cat#RDI-mCD5-53bt      $500.00/0.5mg

                                               FITC : cat#RDI-mCD5-53-FT   $531.00/0.5mg

                                                    PE: cat#RDI-mCD5-53PE     $500.00/0.2mg

                                            PERCP: cat#RDI-mCD5-53PC     $531.00/0.1MG

rev: June 29, 2000 CD5 POLYCLONAl ANTIBODIES

The following antibodies are purified to homogeneity and supplied in 1ml PBS with 0.2% gelatin and 0.1% azide

Protocol For Zinc Fixation:

Fixation in Zinc-Fixative

1. Prepare 0.1M Tris buffer, pH 7.4 (IL)

Tris Base 12.1 g (THAM, TRIZMA)

Deionized water 900 mls

1.o N HCL 81.5 mls

2. Prepare Zinc-Fixative as follows:

0.1M Tris buffer, pH 7.4 recipe above (1000 ml)

0.5g Calcium Acetate

5.0g Zinc Acetate

5.0g Zinc Chloride

Mix to dissolve. The final pH will be approximately

6.5-7.0. Do not readjust the pH, as this will cause

the zinc to come out of solution.

3. Store Zinc Fixative at room temperature.

4. Fix tissues in Zinc Fixative by placing freshly dis-

sected tissue pieces (no larger than 5 mm in size) in

Zinc Fixative and allowing to sit 6 hours at room temp-

erature. After fixation, dehydrate tissues for one hour

each in 70% ethanol, 90% ethanol, 95% ethanol, and 100%

ethanol (2 changes) at room temperature. Tissues are

then cleared in 2 changes of xylene for 50 minutes each

at room temperature, followed by paraffin embedding.

Note: The use of a an alkaline phosphatase detection system may

be needed as this fixation method has been reported to be non-

compatible with blocking of endogenous peroxidase activity.

REFERENCES

Nitta,H. etal. 1997. Improved InSitu Immunodetection of

Leukocytes on Paraffin-Embedded Mouse Spleen. Cell Vision

4: 73-80

Beckstead JH: A simple technique for preservation of

fixation-sensitive antigens in paraffin-embedded tissues. The

Journal of Histochemistry and Cytochemistry 1994;42:1127

Beckstead JH: Letter to the editor. Journal of Histochemistry and

Cytochemistry 1995;43:345 (This is the actual formulation of the

fixative).

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com