anti-MOUSE CD31 antibodies from RDI Division of Fitzgerald Industries Intl

rev: February 4, 2005

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Anti-MOUSE
CD CLUSTERED ANTIBODIES

RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE CD31 Antibodies

see also anti-mouse lyve-1 (new endothelial marker)

DATA SHEET: Rat anti-mouse CD31 (PECAM-1), monoclonal

Catalog#: RDI-mCD31abrt Price: $500.00/1pkg

special-no preservative or carrier, purified ord cat#RDI-mCD31abRT-XP $625.00/vial of 0.5mg

FITC labeled ord cat#RDI-mCD31abrt-FT $625.00/vial 0.5mg

PE labeled cat#RDI-MCD31abrt-PE $625.00/200ug

Biotin labeled cat#RDI-MCD31abrt-BT $562.00/vial

Package Size: 0.5mg in 1ml (0.5mg/ml) buufer and 0.09% (w/v) sodium azide

Supplied: purified (from tissue culture supernatant via chromatography)

Clone: Mec13.3

Immunogen: 129/Sv mouse-derived endothelium cell line tEnd.1

Ig Isotype: Rat IgG2ak (RAT TYPE lew/cR1br)

Reactivity: Reacts with mouse CD31 (Pecam-1) which is expressed constitutively on the surface of endothelial cells from a variety of tissues and weakly expressed on many peripheral lymphoid cells and platelets. This clone inhibits the aggregation of PECAM-1 transfected L-cells and the in vivo aggregation of platelets induced by endothelial injury.

Use: -Flow cytometry:approx 1ug/1 million cells

-Immunohistochemical staining: 5-20ug/ml (acetone fixed frozen and zinc fixed paraffin embedded sections). We do not recommend formalin fixed paraffin sections. (although there are reports of using this clone 10-20ug/ml with formalin fixed paraffin coupled with trypsin or pronase pretreatment 30-40 minutes 37 DEG C, results are inconsistent between labs)

-mouse kidney as pos control. 5-20ug/ml

-blocking experiments (azide free formats)

-immunoprecipitation

Storage: Store at 4 Deg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

ref: Eur. J. Cell Bio. 63:247-254

AM. J. Pathol. 145:33-36.

Am. J. Pathol 151:671-677

Biocell 21:39-46

J. Immunol 158:4872-4878

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product.

Protocol For Zinc Fixation:

Fixation in Zinc-Fixative

1. Prepare 0.1M Tris buffer, pH 7.4 (IL)

Tris Base 12.1 g (THAM, TRIZMA)

Deionized water 900 mls

1.0 N HCL 81.5 mls

2. Prepare Zinc-Fixative as follows:

0.1M Tris buffer, pH 7.4 recipe above (1000 ml)

0.5g Calcium Acetate

5.0g Zinc Acetate

5.0g Zinc Chloride

Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not readjust the pH, as this will cause the zinc to come out of solution.

3. Store Zinc Fixative at room temperature.

4. Fix tissues in Zinc Fixative by placing freshly dissected tissue pieces (no larger than 5 mm in size) in Zinc Fixative and allowing to sit 6 hours at room temperature. After fixation, dehydrate tissues for one hour each in 70% ethanol, 90% ethanol, 95% ethanol, and 100% ethanol (2 changes) at room temperature. Tissues are then cleared in 2 changes of xylene for 50 minutes each at room temperature, followed by paraffin embedding.

REFERENCES

Nitta,H. etal. 1997. Imporved InSitu Immunodetection of Leukocytes on Paraffin-Embedded Mouse Spleen. Cell Vision 4: 73-80