rev: June 22, 1999

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD30  Antibodies

Catalog#: RDI-MCD30-2D    $281.00

Package Size: 100ug in 0.2ml (0.5mg/ml) with 0.1% NaN3

Clone: mcd30.1 (also known as 2SH12-5F-2D)

Ig Isotype: Armenian Hamster IgG group 1

Immunogen: fusion protein of mouse CD30-mIgG1

Reactivity: reacts with CD30, a member of the TNFR family. The CD30 moleculae is predominantly expressed by activated T cells, with its expression peaking at day 4-5 on spleen cells activated with plate bound anti-CD3E ab. The mCD30.1 antibody reacts with a majority of CD8+ T cells, as well as some CD4+ T cells in these cultures. By northern blot analysis, mouse CD30 mRNA is detected in the thymus and in pokeweed mitogen-and Con A- activated spleen cells, but not in the lung, brain, kidney, liver, bone marrow, unactivated spleen, or LPS-activated splenocytes. Reports suggest that signaling through the CD30 molecule may be important in cytokine production by CD8+ CTL lines and may play a role in the negative selection of thymocytes Members of the TNFR family and their ligands are involved in the inducation of diverse biological responses in lymphocytes, including differentiation, proliferation, and cellular death. In humans, CD30 was initially identified in Hodgkin and Reed-Sternberg cells in Hodgkin's disease patients and subsequently was found on neoplastic cells of certain types of non-Hodgkin's lymphomas. Stidues with activated human T cells suggest that CD30+ CD45RO+ T cells comprise the major IFN-G and IL-5 producing cells.

Use: -flow cytometry (recommend using Fc blocking steps along with biotin labeled second step reagents with   Streptavidin:PE)

-in vitro activation of CTL cell lines (using   azide free material)

ref: -J. Immunol. 156:442-449 (1996)

        -Cell 84:551-562 (1996)

Storage: Store at 4 Deg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available:

Biotin conjugated          cat#RDI-MCD30-2DBT     $625.00/0.5mg

No Azide formulation   cat#RDI-MCD30-2DXP     $625.00/0.5mg   sterile filtered, low endotoxin )

Mouse anti-Aremenian Hamster:Biotin cat#RDI-HAMIGGCOC     $344.00/0.5mg

-monoclonal cocktail  clones G70-204 & G94-56

-reacts with Aremian Hamster IgG groups 1, 2 and 3 and Syrian hamster IgG group 1. It does not react with hamster IgM abs. Use at approx 2.5-10ug/ml for flow cytoemtry and histochemistry

-Bulk quotes on request

Catalog#: RDI-HAMIGG1K-X     $375.00/vial     $344.00/vial 3+     $312/vial 10+

Package Size: 500 ug purified IGG, group 1 kappa in 0.5 ml of 10mm PBS pH 7.2 and 150mM NaCl. NO preservative or carrier protein added. Sterile filtered. Endotoxin <0.01ng/ug of ab at time of packaging.

Species: hamster IgG, group 1, kappa (armenian)

Clone: A19-3

Immunogen: TNP-KLH

Activity: -specific for the hapten trinitrophenol (TNP) and selected as an isotype control following screening for low background on a variety of mouse spleen and bone marrow suspensions.

Application: -negative control for hamster IgG group 1K, kappa (Aremenian hamster) isotype neutralizing antibodies

-flow cytomtery

Storage: Store at 4 DEG C immediatley upon arrival. Aliquot and avoid frequent warming cycles. Handle under asceptic conditions

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Animal use requires approval of animal welfare committee. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

inquire for formulations with azide or conjugates

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com