rev:   May 24, 2002

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD27 Antibodies

DATA SHEET: Hamster ant-Mouse/Rat CD27   (MONOCLONAL ANTIBODY)

cat# RDI-MCD27abmH $625.00

also available Biotin conj: cat#RDI-MCD27abmH-BT 250.00/100ug

Presentation: 0.5mg in 0.5ml (1mg./ml) buffer (sterile filtered, no azide or carrier, endotoxoin <0.01ng/ug at time of packaging

Clone: LG.3A10

Immunogen: Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA 1

Isotype: Armenian Hamster IgG1

Purification: From TCS by affinity chromatography

SPECIFICITY: Mab from clone LG.3A10 antibody reacts with CD27, a lymphocyte-restricted member of the Tumor Necrosis Factor Receptor family which binds to CD70. The CD27 molecule is a 45-kDa transmembrane glycoprotein which is constitutively expressed by lymphocytes of the T lineage: virtually all thymocytes and over 90% of peripheral T cells bearing both alpha-beta and gamma-delta T-cell receptors. CD27 cooperates with the pre-TCR in mediating thymocyte differentiation and expansion. In addition, one to ten percent of mature peripheral B cells express CD27, and CD27’s role in the differentiation of human plasma cells has been studied. Mouse NK cells, freshly isolated and IL-2 activated, also express CD27. In the bone marrow, CD27 is found on a progenitor population which provides short-term hematopoietic reconstitution. Cells of the myeloid lineage do not express CD27. Cross-linked LG.3A10 mAb has been reported to amplify the proliferative response of purified T lymphocytes to suboptimal mitogenic stimulation 1 and to enhance NK-cell proliferation and IFN-gamma production. In contrast, non cross-linked LG.3A10 mAb inhibits CD3-induced pre-T cell development by interfering with the receptor-ligand interaction. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with a similar population of rat leukocytes.

Storage: at 4 DEG C under aseptic conditions.

USES: -indirect flow cytometry, approx 1ug/million cells

            -immunoprecipitation

            -in vitro T-cell costimulation, in vitro

              NK-cell stimulation

              -immunohistochemical staining of acetone-fixed frozen sections (not tested for paraffin-not recommended).

              Titer for each application

REFERENCES

1. Gravestein, L.A., J.D. Nieland, A.M. Kruisbeek, and J. Borst. 1995. Novel mAbs reveal potent co-stimulatory activity of murine CD27. Int. Immunol. 7: 551 557.

2. Gravestein, L.A., B. Blom, L.A. Nolten, E. de Vries, G. van der Horst, F. Ossendorp, J. Borst, and W.A.M. Loenen. 1993. Cloning and expression of murine CD27: comparison with 4-1BB, another lymphocyte-specific member of the nerve growth factor receptor family. Eur. J. Immunol. 23: 943 950.

3. Gravestein, LA., W. van Ewijk, F. Ossendorp, and J. Borst. 1996. CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development. J. Exp. Med. 184: 675  685.

4. Agematsu, K., S. Hokibara, H. Nagumo, K. Shinozaki, S. Yamada, and A. Komiyama. 1999. Plasma cell generation from B-lymphocytes via CD27/CD70 interaction. Leuk. Lymphoma 35: 219 225.

5. Takeda, K., H. Oshima, Y. Hayakawa, H. Akiba, M. Atsuta, T. Kobata, K. Kobayashi, M. Ito, H. Yagita, and K. Okumura. 2000. CD27-mediated activation of murine NK cells. J. Immunol. 164: 1741 1745.

6. Wiesmann, A., R.L. Phillips, M. Mojica, L.J. Pierce, A.E. Searles, G.J. Spangrude, and I. Lemischka. 2000. Expression of CD27 on murine hematopoietic stem and progenitor cells. Immunity 12: 193 199.

For In Vitro Research Use Only

not be responsible for violations or patent infringements which may occur with the use or derivation of our products.

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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com