rev: April 14, 2003
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Anti-MOUSE
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-MOUSE CD24 Antibodies (see 3 clones)
Catalog#: RDI-mCD24-M1/69
Price: $281.00/1pkg $250.00/pack 3+
Package Size: 0.1mg in 0.2ml (0.5mg/ml) 10mM phopshate buffer pH7.2 with
150mM NaCl and 0.09% sodium azide.
Supplied: purified rat monoclonal
Clone: M1/69
Ig Isotype: rat IgG2b
Reactivity: The M1/69 antibody reacts with CD24(Heat-Stable Antigen, HSA
or HsAg), a variably glycosylated membrane protein found on erythrocytes,
granulocytes, monocytes, and lymphocytes.(1,2,3) Levels of expression of
CD24 vary during differentiation of the T and B cell lineages. In the bone
marrow, hematopoietic progenitors acquire CD24 expression upon commitment
to the B-lymphocyte lineage(4). Immature B cells in the bone marrow and spleen
of adult mice express high levels of CD24, whereas mature peripheral B
lymphocytes express intermediate levels of CD24. (5,6) The level of CD24
expression has been reported to rise upon activation of splenic B cells with
LPS, but not with CD154 (CD40 Ligand).7 The majority of thymocytes express
high levels of CD24, while mature thymic and peripheral T cells do not express
CD24.(8). In contrast, Gd TCR-bearing thymocytes which emigrate to the spleen
are CD24 + (9) Dendritic cells of the thymus and spleen have also been reported
to express CD24, as determined by staining with M1/69 mAb.10,11 CD24 is involved
in the costimulation of CD4 + T cells by B cells,(7) it is a co-inducer of
in vitro thymocyte maturation,12 and it is a ligand of CD62P (P-selectin).(13)
While the monoclonal antibodies 30-F1, M1/69, and J11d all react with CD24,
they show subtle differences in the level of staining of different lymphocyte
populations.(6) When possible, investigators should continue to use the same
monoclonal antibody as used in previous studies.
Uses: -immunofluorescent staining (1 µg/million cells) with flow cytometric analysis
-immunohistochemical staining of citrate-pretreated formalin-fixed paraffin-embedded sections (0.5 - 2.5 µg/ml)
-western blot analysis,
-in vitro induction (plate-bound in the presence of anti-TCR chain mAb H57-597, of thymocyte maturation,12 complement-mediated cytotoxicity,(14)
-immunohistochemical staining of acetone-fixed frozen and zinc-fixed paraffin-embedded sections.
Storage: Store at 4 Deg C.
REFERENCES
1. Eur. J.Immunol. 8: 539 - 551.
2. J. Biol. Chem. 268: 22345 - 23352.
3. Alterman, L.A., I.N. Crispe, and C. Kinnon. 1990. Characterizationof the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies. Eur. J. Immunol. 20: 1597 - 1602.
4. Hunte, B.E., M. Capone, A. Zlotnik, D. Rennick, and T.A. Moore. 1998. Acquisition of CD24 expression by Lin - CD43 + B220 low ckit hi cells coincides with commitment to the B cell lineage. Eur. J. Immunol. 28: 3850 - 3856.
5. Allman, D.M., S.E. Ferguson, V.M. Lentz, and M.P. Cancro. 1993. Peripheral B cell maturation. II. Heat-stable antigen hi splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells. J. Immunol. 151: 4431 -4444.
6. Stall, A.M., and S.M. Wells. 1997. FACS analysis of murine B-cell populations. In Weir's Handbook of Experimental Immunology, L. A. Herzenberg, D. M. Weir, L. A. Herzenberg and C. Blackwell, eds. Blackwell Science Publishers, , p. 63.1 - 63.17.
7. Kennedy, M.K., K.M. Mohler, K.D. Shanebeck, P.R. Baum, K.S. Picha, C.A. Otten-Evans, C.A. Janeway Jr., and K.H. Grabstein. 1994. Induction of B cell costimulatory function by recombinant murine CD40 ligand. Eur. J. Immunol. 24: 116 - 123.
8. Wilson, A., L.M. Day, R. Scollay, and K. Shortman. 1988. Subpopulations of mature murine thymocytes: properties of CD4 - CD8 + and CD4 + CD8 - thymocytes lacking the heat-stable antigen. Cell Immunol. 117: 312 - 326.
9. Kelly, K.A., M. Pearse, L. Lefrancois, and R. Scollay. 1993. Emigration of selected subsets of .d + T cells from the adult murine thymus. Int. Immunol. 5: 331 - 335.
10. Vremec, D., M. Zorbas, R. Scollay, D.J. Saunders, C.F. Ardavin, L. Wu, and K. Shortman. 1992. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J. Exp. Med. 176: 47 - 58.
11. Ardavín, C., L. Wu, I. Ferrero, and K. Shortman. 1993. Mouse thymic dendritic cell subpopulations. Immunol. Lett. 38: 19 - 25.
12. Cibotti, R., J.A. Punt, K.S. Dash, S.O. Sharrow, and A. Singer. 1997. Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals. Immunity 6: 245 - 255.
13. Aigner, S., M. Ruppert, M. Hubbe, M. Sammar, Z. Sthoeger, E.C. Butcher, D. Vestweber, and P. Altevogt. 1995. Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin. Int. Immunol. 7: 1557 - 1565.
14. Veillette, A., J.C. Zúñiga-Pflücker, J.B. Bolen, and A.M. Kruisbeek. 1989. Engagement of CD4 and CD8 expressed on immature thymocytes induces activation of intracellular tyrosine phosphorylation pathways. J. Exp. Med. 170: 1671 - 1680.
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
Also available: Biotin labeled cat#RDI-mCD24-M169BT $562.00/vial
FITC labeled cat#RDI-mCD24-M169 FT $562.00/vial
For Research Use Only-Not for use in Diagnostics
Catalog#: RDI-mCD24-J11d Price: $562.00/1pkg|
Package Size: 0.5mg purified ab in Tris Hcl
Supplied: purified rat monoclonal
Clone: J11D
Ig Isotype: rat IgM
Reactivity: Reacts with the heat stable antigen (HSA) found on erythrocytes,
granulocytes, monocytes, and lymphocytes. There are variable levels of expression
of HSA, possibly developmentally regulated among subsets of B cells. The
majority of thymocytes express hgh levels of HSA, while mature hymic and
peripheral T cells do not express HSA. In Vitro treatment with J11d antibody
plus complement reduces primary IgM responses and proliferative responses
to LPS.
Uses: -indirect flow cytometry
-complement mediated depletion
-western blot
-histochemistry (acetone fixed frozen sections)
ref: -Eur J. Immunol 20:1597-1602
-J. immunol 127:2496-2501
Storage: Store at 4 Deg C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
Catalog#: RDI-mCD24-30F1 Price: $281.00/1pkg
Package Size: 0.1mg purified ab
Supplied: purified rat monoclonal
Clone: 30-F1
Ig Isotype: rat IgG2c
Reactivity: Reacts with the heat stable antigen (HSA) found on erythrocytes,
granulocytes, monocytes, and lymphocytes. There are variable levels of expression
of HSA, possibly developmentally regulated among subsets of B cells.
The majority of thymocytes express high levels of HSA, while mature
thymic and peripheral T cells do not express HSA. Different staining patterns
may be observed when using clones J11d, 30-F1 and M1/69). Clone 30F1 has
been used in conjunction with anti-CD45R (clone B220), and anti-CD43
(clone S7) to identify defined stages of pro- and pre-B cell development.
Uses: -indirect flow cytometry
-histochemistry (acetone fixed frozen sections)
ref: -Stall, Am and SM Wells, 1997. Facs analysis of murine B-cell populations in Weir's Handbook of Experimental Immunology, fifth edition, Blackwell Science Publishers pp 63.1-63.17
-J Exp Med 173:1213-1225
-J Exp Med 178:951-960
Storage: Store at 4 Deg C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
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RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com