rev: August 4, 2004
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Anti-MOUSE
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-MOUSE CD18 Antibodies
DATA SHEET: Hamster anti mouse B2 Integrin (CD18), monoclonal
Catalog#: RDI-MCD18
Price: $562.00/1pkg
Package Size: 0.5mg in 0.48ml (1.05mg/ml) in PBS (no carrier or preservative
added). Sterile filtered, Endotoxin <3EU/mg
Clone: 2E6
Ig Isotype: Armenian Hamster IgG
Purification: Purified from ascites
Reactivity: Reacts with mouse 90-95 kD common beta subunit of LFA-1 (CD18).
Recognizes the beta subunit in conjunction with all alpha subunits, CD11a,b,c.
This clone will completely block the homotypic aggregation of activated B
cells. Also blocks the heterotypic aggregation between B cell blasts and
T cells in a primary mixed lymphocyte reaction. The binding of opsonized
red cells to macrophages, mediated by C3bi and CR3 are also blocked by clone
2E6.
USE: flow cytometry
blocking experiments
immunoprecipitation
ref: -Bashir, R. et al 1992, J
. Neuroooncol. 12 (2):103.
-Metlay, J. P. et al, 1990.,J. Exp. Med. 171(5):1753.
Storage: Store at -20 Deg C. Aliquot. Avoid frequent freeze thaw cycles.
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
Catalog#: RDI-MCD18-M182 $281.00/100ug with 0.09% sodium azide
also available azide free: cat#RDI-MCD18-M182XP $625.00/0.5mg (No
BSA/No Azide)
Package Size: 0.1mg in 0.2ml with 0.09% NaN3
Clone: M18/2
Ig Isotype: Rat IgG2ak
Antigen: Mouse CTL glycoproteins purified on lentil-lectin columns
Purification: Purified by affinity chromatography
Reactivity: Reacts with common beta2 subunit of LFA-1 (CD11A/CD18), Mac-1
(CD11b/CD18) and gp150,95. It has been reported that clone M18.2 antibody
blocks in vivo metastasis of the LB lymphoma to the spleen and that blocks
in vitro formation of aggregates of LB cells and splenocytes. However, other
reports indicate this mab does not block CTL-mediated killing or adherence
of C3bi sensitized erythrocytes to MAC-1, does not inhibit antigen-specific
binding of T cells to APC and does not block rejection of cardiac allografts.
Based on in vitro studies, it may stimulate adhesion of MAC-1 to its ligand
C3bi and ICAM-1 and stimulate adhesion of LFA-1 to ICAM-1, but it has no
effect upon the interactions of LFA-1 with ICAM-2 or ICAM-3.
USE: -flow cytometry
-histochemistry (acetone fixed frozen and paraffin embedded sections)
-immunoprecipitation
-western blot
-blocking or stimulation of some cell- adhesion functions (azide free formulation- for routine blocking see clone GAME-46)
ref: -J Exp Med 158:586-602
-J. Immunol 150:4466-4477
-Intl Immunol 6:917-924
-J. Leukoc Biol 60:758-765
Storage: Store at 4 Deg C.
Precautions:For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics. Sodium azide may form explosive compounds in
presence of heavy metals or under acidic conditions. Flush drains with copious
amounts of water to prevent buildup of explosive compounds.
Catalog#: RDI-MCD18-GME $281.00/100ug in 0.2ml with 0.09% sodim azide
also available azide free: cat#RDI-MCD18-M182XP $625.00/0.5mg (NO AZIDE)
Package Size: 0.1mg in 0.2ml with 0.1% NaN3
Clone: GAME-46
Ig Isotype: Rat IgG1
Antigen: LFA-1 (CD11A/CD18) affinity purified
Purification: Purified by affinity chromatography
Reactivity: Reacts with common beta2 subunit of LFA-1 (CD11A/CD18), Mac-1
(CD11b/CD18) and gp150,95. Its expression is limited to leukocytes where
it is widely distributed in consort with each of the three integrin alpha
subunits (CD11A, CD11b< CD11c). The B2 integrins are important mediators
of leukocyte-endothelium interactions. It has been reporte dthat clone GAM-46
MAb blocks in vitro adhesion of LFA-1 expressing cells to the ligands ICAM-1,
ICAM-2 and ICAM-3 and of MAC-1 expressing cells to ICAm-1, C3bi and fibrinogen.
USE: -flow cytometry
-histochemistry (acetone fixed frozen sections)
-immunoprecipitation
-blocking of CD18 mediated cell-adhesion functions(azide free formulation)
ref: -J Leukoc Biol. 60:758-765
-Nature 346:425-434
Storage: Store at 4 Deg C.
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
-clone C71/16, rat IgG2ak
cat#RDI-MC18-C71BT $344.00/0.1mg Biotin cong
cat#RDI-MCD18-C71FT $625.00/0.5mg FITC copnjugated
cat#RDI-MCD18-C71PE $469.00/01mg PE conj
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RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266