rev: December 1, 2003
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CD CLUSTERED ANTIBODIES
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Catalog#: RDI-MCD162P-2PX $625.00 $594.00/pack 3+
Package Size: 0.5mg in 0.5ml PBS (No BSA/No Azide) 0.2micron filtered
als oavailable PE conjugated with 0.09% sodium azide cat#RDI-MCD162P-2PE $594.00/200ug
Ig Isotype: Rat IgG1k (Lewis strain)
Immunogen: ovalbumin conjugated peptide covering amino acids 42 to 60 of mouse PSGL-1
Reactivity: Reacts with the N-terminus of mouse CD162 (P- selectin glycoprotein ligand-1, PSGL-1). PSGL-1 is expressed on the cell surface as a homodimer of approx 230kDa. In the moluse, CD162 mRNA is detected in most tissues with high levels found in hematopoietic cells, brain, and adipose tissue. In humans, flow cytometric analyses have revealed CD162 expression on most neutrophils, monocytes and lymphocytes. PSGL-1 is a ligand for P- selectin (CD62P) and is involved in leukocyte rolling and the migration of leukococytes into flammed tissues. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that pther ligands for PSGL-1 and CD62P may exist. Clone 2PH1 Mab is reported to block binding of mouse leukocytes to CD62P.
Use: -histochemistry(acetone fixed frozen sections, and citrate buffer high temp release methods for paraffin sections),
-blocking of ligand binding (azide free
ref: -Blood 90:1934-1942 (1997)
-Blood 87:4176-4186 (1996)
Storage: Store at 4 Deg C.
Precautions: For In vitro research Use Only. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
also available: P-selectin-IgG fusion protein cat#RDI-CD62PFU-AG $406.00/100ug ,
-composed of the signal sequence, the lectin domain, the EGF-like repeat, asn the first two complement-binding doamins of mouse P- selectin (CD62P) fused to the Fc region (hinge, C1 and C2) of human IgG1. This chimeric protein binds to P-selectin expressed on mouse and human neutrophils and myeloid cell lines. In addition, P- selectin Ig has been shown to stain most T-helper-1 (Th1) cells strongly, while T-helper-2 (TH2) cells remain unreactive. Consequently, binding of P-selectin-Ig can be useful in distinguishing the two th subsets.
ref: J. Cell Biol 121:655-664 (1993)
J Cell Biol 125:471-481 (1994)
--Balb/c splenocytes were activated by culture in 4ug/ml Con A for 72 hours. The activated cells were then cultured in wells coated with BSA or with 2.5ug/ml well of P-selectin-IgG fusion protein. Prior to initation of culture, 10ug/ml of an isotype control IgG or 10ug/ml of clone 2HP1 (azide free) was added to the cells. Alternatively, 10ug/ml of the anti-P-selectin Mab clone RB40.34 was added to the coated wells prior to the addition of cells. After a 2 hour incubation, percent cell adhesion was determined. Clone 2PH1 significantly reduces cell adhesion .
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