rev: January 13, 2003
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Anti-MOUSE
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-MOUSE CD157
Antibodies
Catalog#: RDI-MCD157-BP3 $344.00/1pkg
Package Size: 0.1mg in 0.2ml (0.5mg/mL) buffer with 0.09% sodium
azide.
Clone: BP-3
Antigen: BALB/c mouse derived neoplastic pre-B cell line 18.81
Ig Isotype: Mouse (Mus spretus) IgG2Bk
Purification: The antibody was purified from tissue culture supernatant by
affinity chromatography.
Reactivity: The BP-3 antibody reacts with CD157, also known as the BP-3
alloantigen or Bone marrow STromal cell antigen-1 (BST-1),1 found in a variety
of common laboratory strains of mice (AKR, BALB/c, CBA/N, C3H/HeJ, C57BL/6,
DBA/2, NZB, C.B-17, SJL and Swiss, but not A/J).1 CD157 is a differentially
glycosylated GPI-linked cell-surface glycoprotein with a core protein of
32 kDa and differing patterns of glycosylation in different types of cells.
It is a member, along with CD38, of a family of genetically-related ectoenzymes.
The BP-3 alloantigen is expressed on the B lineage from the earliest detectable
progenitors to circulating immature B lymphocytes, on immaturethymocytes
up to the expression of the T-cell receptor, on circulating neutrophils,
peritoneal macrophages, on subpopulations of stromal cells in peripheral
lymphoid organs (but not in bone marrow or thymus), on intestinal epithelial
cells, and in the lumen of kidney-collecting tubules. The distribution of
CD157 antigen on leukocytes and stromal cells of lymphoid organs suggests
that it may be involved in leukocyte-stroma interactions which support the
development, migration, and/or responses of leukocyte populations.
*Note: It has been determined that the binding to B cells may be non-specific via binding FCgamma receptors. By preincubating with the CD16/CD32 MAB , binding is eliminated.
USE: flow cytometry (approx 1ug/1 million cells)
-NOTE:Non specific binding should be blocked with CD16/CD32. For optimal staining of peripheral lymphoid tissue, we recommend the an isotype-specific second-step antibody, and amplification of the staining signal by use of a biotinylated second-step antibody (such as biotinylated anti-mouse IgG 2b mAb R12-3, Cat. no. RDI-MIGG2B-R2BT $406.00) followed by a Streptavidin-PE (RDI-STREPPE $375.00/0.5mg).
-immunoprecipitation
-immunoaffinity chromatography
ref: 1. McNagny, K.M., P.-A. Cazenave, and M.D. Cooper. 1988. BP-3 alloantigen. A cell surface glycoprotein that marks early B lineage cells and mature myeloid lineage cells in mice. J. Immunol. 141: 2551 - 2556.
2. McNagny, K.M., R.P. Bucy, and M.D. Cooper. 1991. Reticular cells in peripheral lymphoid tissues express the phosphatidylinositol-linked BP-3 antigen. Eur. J. Immunol. 21: 509 - 515. 3. Dong, C., J. Wang, P. Neame, and M.D. Cooper. 1994. The murine BP-3 gene encodes a relative of the CD38/NAD glycohydrolase family. Int. Immunol. 6: 1353 - 1360.
4. Dong, C., D. Willerford, F.W. Alt, and M.D. Cooper. 1996. Genomic organization and chromosomal localization of the mouse Bp3 gene, a member of the CD38/ADP-ribosyl cyclase family. Immunogenetics 45: 35 - 43.
5. Ngo, V.N., H. Korner, M.D. Gunn, K.N. Schmidt, D.S. Riminton, M.D. Cooper, J.L. Browning, J.D. Sedgwick, and J.G. Cyster. 1999. Lymphotoxin á / and tumor necrosis factor are required for stromal cell expression of homing chemokines in B and T cell areas of the spleen. J. Exp. Med. 189: 403 - 412.
6. Itoh, M., K. Ishihara, T. Hiroi, B.O. Lee, H. Maeda, H. Iijima, M. Yanagita, H. Kiyono, and T. Hirano. 1998. Deletion of bone marrow stromal cell antigen-1 (CD157) gene impaired systemic thymus independent-2 antigen-induced IgG3 and mucosal TD antigen-elicited IgA responses. J. Immunol. 161: 3974 - 3983.
Storage: Store at 4 Deg C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com