rev:   October 3, 2001

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RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD154/CD40L Antibodies

DATA SHEET:  Hamster anti-mouse CD154 (CD40 Ligand gp39) , monoclonal

Catalog#: RDI-MCD154-MR1

Price: $500.00/1pkg    BULK ON REQUEST

 -also avialble:Biotin labeled cat#RDI-MCD154BT    $562.00/0.5mg

 -also available:no azide cat#RDI-MCD154-Xp    $625.00/0.5mg

Package Size: 0.5mg in 0.5ml

Supplied: purified ab in Tris with 0.1% NaN3

Clone: MR1

Antigen: activated mouse Th1 clone D1.6

Ig Isotype: Armenian hamster IgG, group 3k

Reactivity: Reacts with mouse gp39 (CD40L, CD154) an accesory moleculae expressed on activated T helper lymphocytes. Clone MR1 inhibits in vitro activation of B lymphocytres by T helper cells by blcking interaction of gp39 with CD40. In vivo treatemnet with clone MR1 antibody blocks the development of collagen induced autoimmune arthritis and inhibits formation of germinal centers and generation of memory B cells.

USE: -flow cytometry (approx 1ug/1 million cells) (on activated peripheral T cells, it is recommended that T cells be purified before activation. Mouse CD154 is transiently expressed on the surfaces of activated normal T cells and certain T cell clones with a maximal level detected 608 hours post activation. Activation with immobilized anti- mouse CD3E is sufficient to induce CD154 expression onb CD4 cells. It has been reported that CD8+ cells express CD154 only in response to PMA/Ionomycin treatment.) -blocking

ref: -J. Immunol. 151:2497-2510

      -Proc Natl Acad Sci USA 89:6550-6554

      -Science 261:1328-1330

      -J. Exp Med 180:157-163

Storage: Store at -20 Deg C. Aliquot. Avoid frequent freeze thaw cycles.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

secondary ab: mouse anti-hamster IgG(cocktail) :Biotin conj cat#RDI-HAMGCOC-BT  $344.00/0.5mg

-clones G70-204 & G94-56 react with armenian hamster IgG groups 1, 2 and 3 and syrain hamster group 2

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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266