rev:   October 3, 2001

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD152/CTLA-4 Antibodies

Catalog#:   RDI-MCD152-9H10

Price:        $469.00/1pkg

-also available:no azide cat#RDI-MCD152-9XP    $625.00/0.5mg

Package Size: 0.5mg in 0.5ml|

Supplied: purified ab in Tris with 0.1% NaN3

Cone: 9H10

Antigen: heat killed Staph A bacteria coated with mouse CTLA-4 human IgG1 fusion protein

Ig Isotype: Syrainhamster IgG, group 2k

Reactivity: Reacts with mouse CTLA4 (CD152 ) which is expressed on activated T lymphocytes 2-3 days after stimulation thrpugh T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization, the Cd152 and Cd28 genes map to the same chromosomal band. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression, at both mRNA and protein levels, appears to be restricted to activated T cells with a maximal surface expression between 48 and 72 hours postactivation. The regulation of CTLA-4 protein expression is complex and can involve changes in the rate of transit between intracellular stores and the cell surface. Whereas CD28 delivers a co-stimulatory signal required for T-cell activation, CTLA-4 appears to be a negative regulator of cell mediated immune responses.

USE: -azide free formulation can be used for in vitro and invivo blocking and promotion of in vitro CTLA- 4 signalling by cross linked antibody.

       -flow cytometry (due to low level of CTLA-4, we strongly recommend a second step process using a Fab fragmented goat anti-syrain hamster IgG:Biotin (cat#RDI-HAMIGGabg-BT  $250.00/0.5ml)  conjugate followed by Streptaivin:PE. Blocking with anti-mouse CD16/32 (fc blocking may also help to reduce non specific staining). Since a large population of CTLA-4 molecule is intracellular, use of a permabiliztion reagent as 0.1% saponin is also recommended.

ref: -J. Exp Med 182:459-465

Storage: Store at 4 DEG C

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

Catalog#: RDI-MCD152-4F10    Price: $469.00/1pkg

 -also available:no azide cat#RDI-MCD152-FXP $625.00/0.5mg

                      PE labeled cat#RDI-MCD152-FPE $469.00/100ug

Package Size: 0.5mg in 1ml Tris with 0.1% NaN3

Clone: UC10-4F10-11

Antigen: mouse CTLA-4 mouse IgG2a fusion protein

Ig Isotype: Armenian hamster IgG, group 1k

Reactivity: Reacts with mouse CTLA4 (CD152 ) which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor. CTLA-4 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization, the Cd152 and Cd28 genes map to the same chromosomal band. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression, at both mRNA and protein levels, appears to be restricted to activated T cells with a maximal surface expression between 48 and 72 hours postactivation. The regulation of CTLA-4 protein expression is complex and can involve changes in the rate of transit between intracellular stores and the cell surface. Whereas CD28 delivers a co-stimulatory signal required for T-cell activation, CTLA-4 appears to be a negative regulator of cell mediated immune responses. |

USE: -azide free formulation can be used for in vitro blocking and immunoprecipitation.

          -flow cytometry (due to low level of CTLA-4, we strongly recommend a second step process using an anti-hamster IgG:Biotin conjugate followed by Streptaivin:PE. blocoking with anti-mouse CD16/32 (fc blocking may also help to reduce non specific staining). Since a large population of CTLA-4 molecule is intracellular, use of a permabilization reagent as 0.1% saponin is also recommended.

ref: -Immunity 1:405-413

Storage: Store at 4 DEG C

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com