rev: October 3, 2001
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CD CLUSTERED ANTIBODIES
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
DATA SHEET: Rat anti mouse CD14, monoclonal
Price: $469.00/1pkg $438.00/3 or more BULK ON REQUEST
Package Size: 0.5mg in 1.0ml Tris with 0.1% NaN3
also available: Fitc labeled cat#RDI-MCD14-C53FT $562.00/0.5mg
no azide cat#RDI-MCD14-C53-1XP $625.00/0.5mg
Antigen: recombinant mouse CD14
Ig Isotype: rat (LOU) IgG1k
Reactivity: Reacts with mouse CD14 on residues 308-322 of the hydrophilic region of mouse CD14/ Clone RmC5-3 detects soluble CD14 in serum of LPS-treated mice in a time dependent manner. Positive staining in flow cytometry with cell lines J774A.1 (mouse macrophage), WEHI-265.1, peritoneal resident macrophages, and LPS-activated Kupffer cells, but not unstimulated splenic macrophages, neutrophils, or blood monocytes. Data suggest that CD14 expression by leukocyte population , as detected by clone rmC5-3, may differ in mice and humans. Peritoneal cells from naive mice, 3 day thioglycollate elicited peritoneal exudate, as well as 4 hour LPS- activated peritoneal cells contain populations of MAC-1 (CD11b) high cells which double stain with rmC5-3 antibody. Preliminary evidence suggest that CD14 may be upregulated on mouse blood neutrophils.
USE: flow cytometry (approx1ug/1 million cells)
-NOTE:Non specific binding should be blocked with pure mouse IgG at 10-100 fold excess blocking experiments
ref: -J. Exp. Med 179:1671-1676
-Intl Immunol 3:205-213
Storage: Store at 4 Deg C.
Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
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