rev:    July 12, 2004

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD13 Antibodies

DATA SHEET: Rat anti mouse CD13, monoclonal :FITC conjugated

Catalog#: Fitc labeled cat#RDI-MCD13-R3FT    $406.00/100ug

            -also available PE conjugated: cat#RDI-MCD13-R3PE  $469.00/100ug

Package Size: 0.1mg in 0.2ml (0.5mg/mL) 10mM phosphate   buffer pH7.2 with 150mM NaCl and 0.09%   sodium azide.

Clone: R3-242

Antigen: crude amiopeptidase N preparation from Balb/c intestinal epithelium

Ig Isotype: rat (LOU) IgG1k

Reactivity: Clone R3-242 antibody reacts with aminopeptidase N (APN) which has been reported to be the mouse CD13 homologue. APN is widely expressed in a variety of mammalian tissues, including intestinal and renal epithelial brush border, endothelium, liver, lung, and brain. In lymphoid tissues, clone R3-242 mab is reported to detect APN on monocytes, macrophages, dendritic cells and B lymphocytes, but not on T cells and thymocytes.

*Note: It has been determined that the binding to B cells may be non-specific via binding FCgamma receptors. By preincubating with the CD16/CD32 MAB , binding is eliminated. This effect is in agreement with a previous report using a different antibody to mouse APN (see clone ER-BMDM1). Clone R3-242 mab does not inhibit the enzymatic activity of APN.

USE: flow cytometry (approx 1ug/1 million cells)

        -NOTE:Non specific binding should be blocked with CD16/CD32 ab.

ref: -Eur J. Immunology 23:2358-2364

      -Eur J. Immunol 22:1567-1572

Storage: Store at 4 Deg C.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

see also clone ER-BMDM-1 (mouse macrophages)

DATA SHEET: Rat anti-mouse Macrophage-aminopeptidase), CD13?   monoclonal

Catalog#: RDI-ERBMDM1  or RDI-T2015X

Price: $565.00/vial     100ug


Monoclonal Antibody  ER-BMDM1

Product Information

Rat anti mouse CD13 (aminopeptidase), dendritic cells

ER-BMDM1 is a useful marker for the identification of aminopeptidase N positive macrophages, interdigitating cells and dendritic cells. It is also very suitable for in vitro monitoring of M-CSF stimulated bone marrow cell cultures, as the antigen is gradually expressed with macrophage development. The expression of the ER-BMDM1 aminopeptidase arises after the monocytic stage of differentialtion: bone marrow cells and peripheral blood monocytes are ER-BMDM1 negative, whereas virtually all thioglycollate elicited peritoneal exudate macrophges bind the antibody. The CD designation is based on similarity in molecular and functional characteristics.

Applications: Histology on frozen or formalin fixed sections, FACS

Lot number:

TECHNICAL CHARACTERISTICS

Species: Rat

Class/Subclass: IgG2a

Quantity: 100ug

Purity/Physical state: Purified IgG

Physical state: lyophilized

Reconstitute in: 0.5ml distilled water (= stock solution)

Buffer: Phosphate buffered saline, pH 7.2

Preservative: 0.01% Thimerosal

Stabilizer: 1% BSA

Storage: Aliquots of stock solution can be kept frozen at -70°C; do not freeze working dilutions.

Stability of stock solution: 1 year at -70°C

Approximate working dilution: 1mg/ml (1:200) freshly prepared for immunohistology

This product does not require protease or microwave treatment for the staining of paraffin sections.

Caution: this product contains Thimerosal, a poisonous and hazardous substance

For laboratory use and research purposes only

REFERENCES:

Leenen, P.J.M. et al.: The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidas activity. Eur. J. Immunol. 22, 1567-72 (1992)

Allaerts, W. et al.: A population of interstitial cells in the anterior pituitary with a hematopoietic origin and a rapid turnover: a relationship with folliculo-stellate cells? J. Neuroimmunology 78: 184 197 (1997)

Leenen, P.J.M. et al.: Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers and subpopulation turnover. J. Immunol. 160: 2166-73 (1998)

Specificity Mouse: Mature macrophages

Other species: unknown

Biochemistry

The antigen is a 160kDa membrane associated protein which shows aminopeptidase N activity. It is homologous to the human CD13 marker.

Antigen Distribution

Isolated Cells:

The antigen is present on the majority of isolated dendritic cells of the spleen and lymph node. Over 80% of thioglycollate elicited peritoneal exudate macrophages also express the ER-BMDM1 related antigen. It is absent from freshly isolated bone marrow cells and blood cells.

Tissue Sections:

Lymphoid organs: macrophages surrounding small blood vessels, interdigitating cells, subpopulation of macrophages in the T-cell areas, capsular and medullary cord macrophages in lymph nodes.

Non-lymphoid organs: subpopulation of macrophages (mainly in connective tissues) and dendrocytes, structures positive for aminopeptidase such as the brush border of the small intestine, bile canaliculi in the liver and tubuli and glomeruli in the kidney or type II pneumocytes in the lung. Kupffer cells are negative.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
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RDI Division of Fitzgerald Industries Intl

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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com