rev: May 12, 2004
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Anti-MOUSE
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-MOUSE CD127 (IL-7RA) Antibodies
clone B12-1 (biotin labeled) cat#RDI-MCD127-B12BT $562.00/0.5mg
clone SB/199 (PE labeled) cat#RDI-MCD127-19PE
$594.00/0.5mg
Catalog#: RDI-MCD127-14 $625.00
Package Size: 500 micrograms in 0.5ml (1mg/ml) buffer . no preservative, 0.2micron filtered, endotoxin <0.01ng/ug protein
Clone: SB/14
Anigen: Balb/c mouse pre-B cell line 1A9
Ig Isotype: rat IgG2ak
Reactivity: The SB/14 monoclonal antibody reacts with CD127, the 65-75 kDa type-I transmembrane protein IL-7R. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7R and CD132, the common ¶ chain.3 CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4 - CD8 - immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities. IL-7R is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4 - CD8 - double-negative and CD4 + and CD8 + single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density gd TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring AB TCR-bearing T cells.
-Clone SB/14 Mab has been reported to neutralize IL-7 induced proliferation of the IL-7 dependent immature B-lymphocyte line 2E8 (dteceted by BRDU incorporation)
Uses: -immunofluorescent staining and flow cytometry (recommend 3 step staining protocol)
-blocking experiments
-not recommended for WB or IP or histochemistry
Storage: Store at 4 Deg C under aseptic conditions
REF: -Blood 93:140-148 (1999)
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
DATA SHEET: Rat IgG2a Isotype Control (No Azide, sterile filtered)
Catalog#: RDI-RTIGG2A-X $438.00/vial
Package Size: 500 ug purified IgG (via Protein G) in 0.5 ml of 10mm PBS pH 7.2 and 150mM NaCl. NO preservative or carrier protein added. Sterile filtered. Endotoxin <0.01ng/ug of ab at time of packaging.
Species: rat IgG2ak
Clone: R35-95
Strain of Origin: Lou
Activity: -this clone has unknown specificity but has been tested by HPLC, SDS-PAGE, ELISA and immunofluorescence staining of mouse spleen and bone marrow suspensions and immunohistochemical staining of acetone fixed-frozen and paraffin embedded sections to assure purity, isotype and lack of tissue reactivity.
Application: -negative control for rat IgG2ak isotype neutralizing antibodies
-flow cytomtery
Storage: Store at 4 DEG C immediatley upon arrival. Avoid frequent warming cycles. Handle under asceptic conditions
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Animal use requires approval of animal welfare committee. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.
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RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com