rev: March 26, 2003
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Anti-MOUSE
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-MOUSE CD124 (IL-4 RA)
Antibodies
cat# RDI-MCD124-M1 $438.00.00/0.1mg - DISCONTINUED
RDI-MCD124-MBT $500.00/0.1mg Biotin - DISCONTINUED
RDI-MCD124-M1XP $688.00/0.5mg no azide - DISCONTINUED
Presentation: 0.1mg in 0.2ml(0.5mg/ml) buffer with 0.09% sodium
azide
Clone: mIL4R-M1
Isotype: Rat IgG2a
Immunogen: CTLL-19.4 cells, a subclone of CTLL-2 cells, that express high
levels of IL-4R .
Purification: purified from tissue culture supernatant via Protein G column
chromatography.
Specificity: The mIL4R-M1 monoclonal antibody specifically binds to the alpha
subunit of the mouse Interleukin-4 Receptor (IL-4R , aka, CD124).1 The mIL4R-M1
antibody does not recognize human or rat IL-4R; crossreactivities with IL-4R
from other species have not been established.
Activity: -Immunofluorescent staining and Flow Cytometric Analysis: The purified form of the mIL4R-M1 antibody can be used for the immunofluorescent staining (1 µg antibody/10 6 cells) and flow cytometric analysis of nucleated mouse cells to measure their expressed levels of surface IL-4R A three-layer staining, with anti-rat IgG:Biotin followed by Streptavdin:PE
-Neutralization: The mIL4R-M1 antibody reportedly blocks IL-4’s binding to and biological effects on IL-4R-positive cells. use azide free material
-Immunoprecipitation: The mIL4R-M1 antibody is reported to immunoprecipitate mouse IL-4R proteins.
-ELISA: The purified mIL4R-M1 antibody is reported to be useful for a sandwich ELISA that measures soluble mouse IL-4R protein levels.9 The mIL-4R-M1 antibody can be paired with the mIL4R-M2 (rat anti-mouse sIL-4R) antibody and recombinant soluble mIL-4R as the standard.
Storage: Store 2-8 DEG C. Avoid frequent warming cycles.
Background: The mouse IL-4R is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines.1-3 The cell surface IL-4R chain binds IL-4 with high affinity and associates with either the common ã chain (IL-4R /c; aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4R /IL-13R ; aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes (reviewed in reference 4). The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4’s biological activities.1 The mIL4R-M1 antibody also recognizes naturally-occurring, solu-ble truncated forms of IL-4R (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4R domain or from differential mRNA splicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. 1,2
REFERENCES
1. Beckmann, M. P., K. A. Schooley, B. Gallis, T. Vanden Bos, D. Friend, A. R. Alpert, R. Raunio, K. S. Prickett, P. E. Baker, and L. S. Park. 1990. Monoclonal antibodies block murine IL-4 receptor function. J Immunol 144:4212-4217.
2. Mosley, B., M. P. Beckmann, C. J. March, R. L. Idzerda, S. D. Gimpel, T. VandenBos, D. Friend, A. Alpert, D. Anderson, J. Jackson, J. M. Wignall, C. Smith, B. Gallis, J. E. Sims, D. Urdal, M. B. Widmer, D. Cosman, and L. S. Park. 1989. The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms. Cell 59:335-348.
3. Lowenthal, J. W., Castle, B. E., Christiansen, J., Schreurs, J., Rennick, D., Arai, N., Hoy, P., Takebe, Y., and M. Howard. 1988. J Immunol 140, 456-464.
4. Gessner, A., and M. Rollinghoff. 2000. Biologic functions and signaling of the interleukin-4 receptor complexes. Immunobiology 201:285-307.
5. Feldman, G. M., S. Ruhl, M. Bickel, D. S. Finbloom, and D. H. Pluznik. 1991. Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6. Blood 78:1678-1684.
6. Sempowski, G. D., M. P. Beckmann, S. Derdak, and R. P. Phipps. 1994. Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis. J Immunol 152:3606-3614.
7. Hassuneh, M. R., P. S. Nagarkatti, and M. Nagarkatti. 1997. Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin. Blood 89:610-620.
8. Kubo, M., M. Yamashita, R. Abe, T. Tada, K. Okumura, J. T. Ransom, and T. Nakayama. 1999. CD28 costimulation accelerates IL-4 receptor sensitivity and IL-4-mediated Th2 differentiation. J Immunol 163:2432-2442.
9. Chilton, P. M., and R. Fernandez-Botran. 1993. Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4. J Immunol 151:5907.
For In Vitro Research Use Only.
Inquire for an isotype control
DATA SHEET: Rat IgG2a Isotype Control
Catalog#: RDI-RTIGG2A $312.00/vial $281.00/vial 3+
Package Size: 500 ug purified IgG in 1ml of 10mm PBS woth 0.09% sodium azide
Species: rat IgG2ak
Clone: R35-95
Strain of Origin: Lou
Activity: -this clone has unknown specificity but has been tested by HPLC,
SDS-PAGE, ELISA and immunofluorescence staining of mouse spleen and bone
marrow suspensions and immunohistochemical staining of acetone fixed-frozen
and paraffin embedded sections to assure purity, isotype and lack of tissue
reactivity.
Application: -negative control for rat IgG2ak isotype for hisotchemistry -flow cytomtery
Storage: Store at 4 DEG C immediatley upon arrival. Avoid frequent warming cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Animal use requires approval of animal welfare committee. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.
also available without azide cat#RDI-RTIGG2A-X $438.00
inquire for Biotin, FITC and PE conjugates
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RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com