rev:  April 22, 2003

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


Anti-MOUSE CCR5 (CD195)

RAT ANTI- MOUSE CCR5 MONOCLONAL ANTIBODY

cat# RDI-MCCR5-48         $500.00/0.5mg purified

       RDI-MCCR5-48BT    $625.00/0.5mg Biotin conj

Presentation: 0.5mg in 1ml (0.5mg/ml) buffer with 0.09% sodium azide

Clone: C34-3448

Isotype: Rat IgG2c

Immunogen: KLH-conjugated peptide consisting of amino acids 9-30 of mouse CCR5.

PREPARATION: purified from tissue culture supernatant via Protein G column chromatography.

Storage: Store undiluted at 4 DEG C. Avoid frequent freeze thaw cycles

Use: indirect flow cyctometry (approx 0.25ug/million cells)

-Expression of mouse CCR5 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA (10 µg/ml, final concentration; Sigma). The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 (100 U/ml, final concentration. The splenocytes were stained with purified rat anti-mouse CCR5 antibody followed by biotinylated mouse anti-rat IgG2c (clone A92-1, Cat. No. RDI-10142BT $406.00/0.5mg) and treptavidin-PE (RDI-STREPPE $375.00/0.5mg) Isotype control cat#RDI-RTIGG2CX   $406.00/0.5mg

Reactivity: Antibody reacts with mouse chemokine receptor CCR5, a member of the B chemokine receptor family. CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1) and Beta . 1-3 In the mouse system, the gene for CCR5 has been mapped to chromosome 9. 3 CCR5 mRNA is expressed in heart, spleen and liver tissues, and by macrophages and T-lymphocytes.

REFERENCES

1. Napolitano, M., K. B. Seamon, and W. J. Leonard. 1990. Identification of cell surface receptors for the Act-2 cytokine. J. Exp. Med. 172:285-9.

2. Meyer, A., A. J. Coyle, A. E. Proudfoot, T. N. Wells, and C. A. Power. 1996. Cloning and characterization of a novel murine macrophage inflammatory protein-1 receptor. J. Biol. Chem. 271:14445-51.

3. Boring, L., J. Gosling, F. S. Monteclaro, A. J. Lusis, C. L. Tsou, and I. F. Charo. 1996. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1 receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J. Biol. Chem. 271:7551-8.

For Research Use Only. Not for use in or on humans or animals, not for use in diagnostics. RDI will not be held  responsible for patent infringement or other violations that may occur with the use of our products.

Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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