rev:   February 4, 2004

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(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

XANTHINE OXIDASE (see below 2antibodies)
Mouse anti-Xanthine Oxidase/Aldehyde Oxidase

cat# RDI-Xanthabm         $438.00/100ug

        RDI-XANTHabm-1  $688.00/200ug

        RDI-XANTHabm-BT  $562.00/100ug Biotin coj

Presentation: Protein A purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml -no BSA cat#RDI-XANTHabm-1XP $750.00/200ug

clone: 145.5

Isotype: mIgG1

Immunogen: his-tagged C-terminal 358 amino acid fragment of human xanthine oxidase

Reactivity: human,rat, mouse, and rabbit

Uses: -Western blot: 1ug/ml (150kda (XDH) 130kda (A0)

         -immunoprecipitation:2ug/mg protein

       -histochemistry (frozen and paraffin sections)

         12-4ug/ml, paraffin sectiosn require boiling tissue sections in 10mM citrate buffer pH6.0 for 10-20minutes followed by cooling to room temp for 20 minutes.

Positive Ctrl: heptatic, pulmonary, intestinal and renal epithelium, smooth muscle;endothelium;and Kupffer cells in normal tissues

Localization: Cytoplasmic

Background: Recognizes a protein of 150kDa AND 130KDA. THESE CORRESPOND WITH 150KDA FOR A MONOMER OF XANTHINE DEHYDROGENASE(XDH) AND 130KDA FOR ALDEHYDE OXIDASE (AO) AND THE PROTEOLYTICALLY IRREVERSIBLY MODIFIED XANTHINE OXIDASE (XO). In normal tissues the enzyme exists predominantly as XDH which converts hypoxathine and xanthine to uric acid, a critical plasma antioxidant. During ischemia, XDH is reversibly converted to the oxygen radical producing XO by oxidation of essential sulfhydryl groups or irreversibly through limited proteolytically cleavage of the amino-termius. AO has a higher affinity for aldehydes than purines but produces O2- in a manner very similar to XO because both oxidases possess nearly identical internal electron transport systems. XO and AO share sequence identity (>49%), similarity (>84%) and cofactor requirements.

Storage: 2-8 deg c

For Research Use Only

DATA SHEET: Rabbit anti-XANTHINE OXIDASE   (bovine buttermilk)

Catalog#: RDI-XANTHBabr     $312.00/vial

Also available HRP conjugated: cat#RDI-XANTH-HRP  $344.00/20mg

                                Biotin conj: cat#RDI-XANTH-BT     $344.00/10mg

Size: 50 mg (lyophilized)

Antibody Concentration: approx 10mg/ml

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: bovine buttermilk

Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.

Reactivity: This polyclonal reacts with xanthine oxidase from buttermilk showing a 275kda band under non-reducing conditions of SDS-PAGE. Xanthine Oxidase (XO) catalyzes the oxidation of xanthine to uric acid. XO is a unimolecular, multicomponent electron transport system comprised of enzyme, two FAD molecules, two atoms of molybdenum and eight atoms of iron. Cross reactivity with other mammalian forms is expected but not tested.

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049


phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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