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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Vitamin D3 Receptor


Rat anti-Vitamin D Receptor(VDR)   (human/rat/mouse/hamster/pig)

cat# RDI-VITD3Rabrt   $469.00/vial

Background: Vitamin D3 receptor (VDR) has been found in the intestine, bone and kidney as well as skin and many cells of the endocrine and immune systems. VDR is a member of the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucorticoid, androgen, and thyroid hormone receptors. Vitamin D is known to play a role in calcium and phosphorous homeostasis, bone remodeling, and regulation of cellular growth and differentiation.

isotype: Rat IgG2b

clone: 9A7

package: 100ug in 0.1ml (1mg/ml) PBS with 0.05% NaN3

antigen: partially purified chicken intestinal cytoplasmic 1,25 (OH)2D3 receptor protein. Hybridoma formed by fusing splenic lymphocytes derived from these Lewis rats with the myeloma cell line SP2/0-Ag14.

Specificity: -recognizes with high affinity just C-terminal to the DNA binding domain of the VDR; studies suggest the antigenic site lies between amino acids 89 to 105 of human VDR. It reacts with both the occupied and unoccupied forms of the receptor, and binds to VDR from a variety of tissues and cell types.

-recognizes VDR in the intestinal tissue of fish, frog, chick and rat, as well as kidney from rat. It has also shown reactivity with VDR in cultured cells from human breast, human intestine, rat bone, hamster ovary, pig kidney, and mouse fibroblast. It has shown no reactivity with estrogen and glucocorticoid receptors.

Use: -Western blot:1ug/ml starting dilution detects the band approx 52-55kda in mammalian species, but band is approx 58kDa and 60Kda in avian.

-Histochemistry: Bouin's fixative recommended. 5ug/ml (strong nuclear staining)

-immunoprecipitation (titer must be determined)

-gel supershift


Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

Main REF: J.W. Pike et al, Proc Natl Acad Sci USA 79:7719-7723, 1982.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Rabbit anti-Vitamin D Receptor (Human/Mouse/Rat)

cat# RDI-VITDRabRX   $469.00/100ug

Presentation: 100ul of antiserum in PBS with 0.05% sodium azide

Immunogen: Synthetic Peptide: E(395) E H S K Q Y R C L S F Q P E C S M K(413) aa391-413 from human VDR

Isotype: Rabbit IgG

Species Reactivity: Human, mouse, rat (others not tested)

Use: -Western blot: 1:100

        -Immunoprecipitation

        -gel shift

This Pab has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~53 kDa protein representing VDR in COS-7 cells transfected with the human gene. It supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).

ref: Molecular and Cellular Biology, 15: 5789-5799, 1995.

       Molecular Endocrinology, 8: 159-172, 1994.

Background: Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expresion, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription.

Recombinant human VDR and human RXR beta (catalog # RDI-RXRB-AGX) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures


also available: recombinant human Vitamin D3 Receptor   $688.00  $625/package 2+

Vitamin D3 Receptor, Recombinant Human

Product No.  RDI-VITD3R-AG

750 pmol (36 µg)*  Bulk Price Request

* active receptor

Applications

Steroid biochemistry research

Signal transduction research

Drug discovery

Description

Vitamin D3 Receptor (VDR) is a 48 kDa hormone-inducible transcription factor that acts in the regulation of a number of cellular functions. PanVera's Vitamin D3 Receptor is both soluble and functionally active, and tested for its ability to bind [3H]1 alpha,25-dihydroxyvitamin D3. Like other members of the steroid hormone receptor superfamily, VDR binds to appropriate response elements upstream of the regulatable gene. For example, VDR binding to a site upstream of the osteocalcin gene regulates calcium homeostasis.

Specifications

Source: Recombinant baculovirus-infected insect cells

Functional Assay: The amount of active receptor is determined by quantitation of [3H]1 alpha,25-dihydroxyvitamin D3:receptor complexes using a hydroxylapatite (HAP) assay

Concentration: > or = 1,000 pmol [3H]1 alpha,25-dihydroxyvitamin D3:receptor bound per milliliter

Specific Activity: > or = 1,000 pmol [3H]1 alpha,25-dihydroxyvitamin D3:receptor bound per milligram protein

Physical Purity: Greater than 50% as assessed by visualization of 10 µg of protein on a Coomassie® Blue-stained SDS-polyacrylamide gel

Storage Buffer: 50% glycerol, 50 mM Tris (pH 7.5), 500 mM KCl, 2 mM DTT, 1 mM EDTA

For research use only. Not supplied for use in human diagnostic or therapeutic procedures



RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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