rev: October 27, 1998

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-VIP  (polyclonal)
Pab (rabbit) to Vasoactive Intestinal Peptide (VIP)

Cat#RDI-PRO11428     $344.00

Category                  Rabbit polyclonal

Purification/Form    Serum

Immunogen               Porcine VIP

Description/Specificity Located in the nerve fibers in the central and peripheral nervous system, VIP is probably acting as a neurotransmitter. VIP-producing tumors are usually neuroblastomas or endocrine tumors in the pancreas. The localization of the antigen is extracellular and cytoplasmic.

Recommended Positive Control: Ileum

Application Suitable for frozen sections, cell preparations and paraffin sections*

* no pretreatment required

Antigen Recognized in species tested so far: Reactive with most mammals including human

Working Dilution 1:50 - 1:100

Dilution Buffer PBS with 1% BSA, optimal dilution should be tested by serial dilution

Incubation Time 1 h at room temperature

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Volume 0.25 ml; (contains 0.1% sodium azide)

For Research use Only


Polyclonal (Guinea Pig) Antibody to VIP (Vasoactive intestinal peptide)

Cat#RDI-PRO16071      $690.00

Category                          Guinea pig polyclonal

Form/Purification             Undiluted serum (lyoph.)

Immunogen Synthetic          human VIP (, #7161)

Description/Specificity VIP is localized in nerve fibers of the central and peripheral nervous system, and is probably acting as a neurotransmitter. Smooth muscle relaxation, vasodilation and secretion from exocrine glands are some of the effects of VIP. The Verner-Morrison or Watery Diarrhea Hypokaliemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. These VIP-producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.

Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining, while PHI does not.

Positive Control Stefanini-fixed frozen sections of rat intestine

Species tested so far Human, pig, rat and guinea pig

Application · Immunohistochemistry  · Suitable for frozen and paraffin sections

Reconstitution Dissolve the antiserum in 50 - 100 µl distilled water, and dilute further in 0.1 M PBS with 1% BSA and 0.1% NaN3.

Working Dilution 1:1000 - 1:3000 for immunohistochemistry (PAP)

1:1000 - 1:1500 for immunofluorescence with overnight incubation at 2-8°C.

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Quantity / Volume 50 µl (lyoph.)

Reference Messel T et al. Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused porcine ileum. Reg Pept 38, 179-198 (1992)

For Research Use Only


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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