rev:   November 3, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mouse anti-Villin Monoclonal ab

cat# RDI-VILLINabm      $438.00/vial 50ug

       RDI-VILLINabm-1   $750.00/vial 150ug

Background: The isolated intestinal microvillus cytoskeleton (core) consists of four major proteins: actin, villin, fimbrin, and brush border myosin-I. These proteins can assemble in vitro into structures resembling native microvillus cores. Of these components, villin, and brush border myosin-I show tissue-specific expression, so they may be involved in the morphogenesis of intestinal micro- villi. Found in association with the microvillar actin bundles of the intestinal brush border, villin is a 95kDa protein composed of   two very similar domains of approximately 44kDa each, the core, and a C-terminal domain of 8kDa, the headpiece. The core has been shown to contain villin's Ca+2 regulated capping, nucleating, and severing activities, but it cannot induce the formation of microfilament bundles without the headpiece. Villin is a useful differentiation marker of early embryogenesis and may be useful in diagnosis and follow-up of colorectal cancers. It has been demonstrated that villin is necessary for bothe the cytoskeletal and membrane protein organization of a functional brush border.

Antigen: bovine villin

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

Use: -Suitable for western blot on human using approx 0.5ug/ml ab on total cell lysate Of HCT-8 cells. Major band at Mw 95kDa (other bands at approx 44kDa).   Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection.

       -also for use in immunofluorescence and histochemistry

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: Friederich,E., et al. 1990 Bioessays 12:403.

                   Friederich,E., et al. 1992 Cell 70:81.

                   Costa de Beauregard, M.-A., et al. 1995 EMBO J. 14:409

                   Magdalenat, H. 1992 J. Imm. Meth. 150:133.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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