rev:   June 24, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see below monoclonal and polyclonal
DATA SHEET:   Mouse anti-Human VE-CADHERIN

Catalog#: RDI-VECADHabm    $469.00/vial

Package Size: 100ug in 1ml (0.1mg/ml) PBS with 0.1%   BSA and 0.02% NaN3.

Species: mouse IgG2a

Clone ref: BV6

Immunogen: human endothelial cells

Reactivity: react with human VE-Cadherin. Specificity determined by immunoprecipitation from a detergent extract of biotinylated cells. The epitope recognized is located on the extracellular domain of the molecule. Antibody binding requires calcium. Does not react with mouse or bovine VE-Cadherin . Epitope is trypsin sensitive

Uses: -western blotting (non-reducing conditions, 2-5mMCa++ required in buffer,expected size is approx 140kda)

-Flow cytometry (in the presence of CA++, epitope is trypsin sensitive), use PBS + 2-5mM EDTA for cell detachment

-histochemistry

-immunoprecipitation

-ELISA

Titers must be optimized for each application

Storage: Store at 4-8 DEG C

ref: -Lampugnani, G. et al (1992) J. Cell Biol. 118:1511-1522

       -Brevario, F. et al (1995) Arterioscler. Thromb. Vasc. Biol. 15:1229-1239

        -Martin-Padura, I et al. (1995) J Pathol 175:51-57

        -Matsumoto, Y et al (1993)

       Transplantation 56:69-75

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Note: Upon receipt, tighten cap, and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in vial (during shipping, some antibody can become lodged inside the cap and on sides of vial.

Note: Upon receipt, tighten cap, and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in vial (during shipping, some antibody can become lodged inside the cap and on sides of vial.

for anti-mouse see http://www.researchd.com/mscdabs/mscd144.htm


RABBIT ANTI HUMAN/Bovine CD144  VE-cadherin (polyclonal)

cat# RDI-CD144abrx   $438.00

   also available FITC conjugated (with sodium azide and BSA)

               cat#RDI-CD144abrx-FT    $469.00/100Tests in 1ml

Presentation: 0.1mg in 1ml (0.1mg/ml) affinity purified rabbit IgG in PBS (no preservative)

Immunogen: Recombinant protein fragments corresponding to amino acids 1-258 of VE-cadherin.

Immunogen: Antisera to human CD144 were raised by repeated immunisation of rabbits with highly purified antigen.

Specificity: recognises human CD144 (also known as VE-cadherin), a 130KDa cell surface glycoprotein expressed by endothelial cells. It does not cross react with N-cadherin, which is also expressed by endothelial cells. -reported to block adhesive properties of VE-cadherin. (1)

Species: Cross Reactivity Bovine (others not tested)

             - Antibody reactivity and working conditions may vary between species.

Applications: -flow cytometry: approx 1ug/1million cells

                     -histochemistry: frozen sections

                     -Elisa

                    -western blot

                   -functional studies

Storage: Undiluted at -20 DEG C only. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

For Research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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