rev:  October 23, 2002

HOME (index page)

Return (alphabetical   antibody page)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Vasopressin & Vasopressin Receptor    & Neurophysin II (vasopressin precursor)


DATA SHEET: Rabbit anti-Vasopressin (Polyclonal)

cat# RDI-VASOPRESabr   $312.00/100ul

Presentation: 100ul neat rabit sera with 0.1% sodium azide

Immunogen: arginine vasopressin conjugated to thyroglobulin

specificty: reacts with vasopressin (<1% cross with oxytocin)

species reactivity: rat, mouse, rabbit, sheep and human

Applications: -Immunohistochemistry:1:5K-1:10K using 24hour incubation on formalin, Bouin's or Zamboni's fixed tissue. Will react with paraffin embedded tissue.

-Immunocytochemistry: 1:1K-1:200K (24 hour incubation on fetal rat neurons_

-Immunoblotting: 1:1K

-RIA: 1:30K

-Optimum titer must be established by the end user

References: 1)PNAS USA (1989) 86:2952

Storage: Store at -20 DEG C in aliquots. Avoid frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge for 1-2 minutes 1000rpm to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


vasopressin (AVP) exerts its action through binding to specific membrane receptors coupled to distinct second messengers. There are three types of AVP receptors: V1a, V1b and V2 subtypes. The V2 receptor stimulates adenyl cyclase and protein Kinase A, V1 activates phospholipase A2.C and D, resulting in prodcution of IP3 and DAG, the mobilization of intracellular calcium, the influx of extracellular calcium, the activation of protein kinase C, and protein phosphorylation. The V1a receptors mediate vasoconstriction and hepatic gluconeogenesis platelet aggregation, coagluation factor release. V1a receptors are found in vascular smooth muscle, hepatocytes, blood platelets, lymphocytes and monocyte, tyep II pneumocytes, adrenal cortex, brain, reproductive organs, retinal epithelium, renal mesangial cells.

the V1b modulates ACTH secretion from the naterior pituitaary and the V2 mediates anti-diuretic effects. V1b receptors are located in anterior pituitary. V2 renal receptors are present in medullary portion of the kidney where they control free water and urea absorption by stimulating adenyl cyclase.

AVP receptors are members of the G protein coupled receptors with putative 7 transmembrane domains. the sizes of various AVP receptors are V1a (rat, 424aa, human 418aa); V1b(rat, 421aa, human 424aa); V2 (rat, 371aa, human 371aa).  The N terminus and C-terminus are predicted to be extracellular and cytoplasmic, respectively.


DATA SHEET: Rabbit anti-Arginine Vasopressin Receptor (AVPR-V2)   (Polyclonal)

cat# RDI-AVPRV2abr    $500.00/100ug

control peptride cat#RDI-AVPRV2-CP   $188.00/100ug

Presentation: 100ug affinity purified immunoglobulin in PBS with 0.1% BSA & 0.02% merthiolate


Immunogen: an 18 amino acid peptide between the 5th and 6th transmembrane domain of rat AVP-V2 (predicted to be the cytoplasmic domain)

specificty: reacts with the arginine vasopressin receptor (AVP-2) in rat. The peptide used for immunization has no significant simililarity with AVP V1a or V1B.

species reactivity: rat (not tested with other species) by antigen homology (human and mouse expected)

Applications: -western blot:1-10ug/ml with chemiluminescence detection, approx 62kda band in mebranes from rat inner medualla. N-Gluconase redcued the size to approx 36 kda (deglycosylated form)

-histochemistry: 2-10ug/ml on pfa fixed tissue. AVP-V2 has been localized in terminal IMCD and other areas of rat kidney.

-Optimum titer must be established by the end user

References: 1) Nature (1992) 357:336-339

                    2) J.Clin Invest (1995) 96:1768-1778

Storage: Store at -20 DEG C in aliquots. Avoid frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge for 1-2 minutes 1000rpm to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


see also rabbit anti-vasopressin receptor: rat

V1a (extracellular)   cat#RDI-AVPRV1Aabr       $500.00/100ug (rat/mouse)

V1a (intracellular)   cat#RDI-AVPRV1AXabr     $500.00/100ug (rat)

V1B (extracellular)  cat#RDI-AVPRV1Babr       $500.00/100ug (rat, human)


DATA SHEET: Rabbit anti-Arginine Vasopressin Receptor (AVPR-1A)    (Polyclonal)

cat# RDI-AVPRV1Aabr     $500.00/100ug

control peptide cat#RDI-AVPRV1A-CP $188.00/100ug

Presentation: 100ug affinity purified immunoglobulin in PBS with 0.1% BSA & 0.02% merthiolate

Immunogen: an 19 amino acid peptide mapping within the predicted extracellular, N-terminal domain . The peptide was synthesized with a cysteine at the amino terminus and coupled to KLH. Antibodies affinity purified using the control peptide coupled to sepharose.

specificty: reacts with the arginine vasopressin receptors , 394, 395 and 424aa)(AVP-1A) in rat. The peptide used for immunization has no significant simililarity with AVP V1b or V2 or V3 receptors.

species reactivity: rat (not tested with other species) by antigen homology:

19 amino acid immunogen peptide:

-84% homology with mouse (16/19aa)

-68% homology human 13/19aa

-52% homology with sheep (10/19aa)

Applications: -western blot:1-10ug/ml with chemiluminescence detection,

-histochemistry:not tested recommend 2- 10ug/ml on pfa fixed tissue.

References: 1) Nature (1992) 357:336-339

                    2) J.Clin Invest (1995) 96:1768-1778

Storage: Store at -20 DEG C in aliquots. Avoid frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge for 1-2 minutes 1000rpm to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product.


DATA SHEET:   Rabbit anti-Arginine Vasopressin Receptor (AVPR-1A)   (Polyclonal) RAT

cat# RDI-AVPRV1AXabr    $500.00/100ug

control peptide cat#RDI-AVPRV1AX-CP $188.00/100ug

Presentation: 100ug affinity purified immunoglobulin in PBS with 0.1% BSA & 0.02% merthiolate

Immunogen: an 18 amino acid peptide mapping within the predicted cytoplasmic domain between the TM5 and TM6. The peptide was synthesized with a cysteine at the amino terminus and coupled to KLH. Antibodies affinity purified using the control peptide coupled to sepharose.

specificty: reacts with the arginine vasopressin receptors , 394, 395 and 424aa)(AVP-1A) in rat. The peptide used for immunization has no significant simililarity with AVP V1b or V2 or V3 receptors.

species reactivity: rat (not tested with other species) by antigen homology:

18 amino acid immunogen peptide:

-55% homology with mouse (10/18aa)

-no significant homology with human

Applications: -western blot:1-10ug/ml with chemiluminescence detection,

-histochemistry:not tested recommend 2- 10ug/ml on pfa fixed tissue.

References: 1) Nature (1992) 357:336-339

                    2) J.Clin Invest (1995) 96:1768-1778

Storage: Store at -20 DEG C in aliquots. Avoid frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge for 1-2 minutes 1000rpm to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product.


DATA SHEET: Rabbit anti-Arginine Vasopressin Receptor (AVPR-1B)   (Polyclonal) RAT/Human

cat# RDI-AVPRV1Babr    $500.00/100ug

control peptide cat#RDI-AVPRV1B-CP $188.00/100ug

Presentation: 100ug affinity purified immunoglobulin in PBS with 0.1% BSA & 0.02% merthiolate

Immunogen: an 18 amino acid peptide mapping within the predicted 1st extracellular domain of rat AVP-V1b. The peptide was synthesized with a cysteine at the amino terminus and coupled to KLH. Antibodies affinity purified using the control peptide coupled to sepharose.

specificty: reacts with the arginine vasopressin receptors (AVP-1B) in rat. The peptide used for immunization has no significant simililarity with AVP V1a or V2

species reactivity: rat (not tested with other species) by antigen homology:

18 amino acid immunogen peptide:

-88% homology with human (16/18aa

Applications: -western blot:1-10ug/ml with chemiluminescence detection,
-histochemistry:not tested recommend 2- 10ug/ml on pfa fixed tissue.

References: 1) Nature (1992) 357:336-339

                    2) J.Clin Invest (1995) 96:1768-1778

Storage: Store at -20 DEG C in aliquots. Avoid frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge for 1-2 minutes 1000rpm to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

Return (alphabetical   antibody page)

Return  (anti-enzyme antibodies..index)

Ordering terms