rev: December 2, 1999

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


VaChT (see two polyclonal antibodies below)
Rabbit anti-Vesicular acetylcholine Transporter (VAChT) polyclonal

Cat#RDI-PRO16087       $562.00/vial

Category                             Rabbit polyclonal

Purification/Form               Rabbit serum

Immunogen                          Synthetic peptide from the C-terminus of rat VAChT conjugated to BSA

Description/Specificity       The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylchiolinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of rat and mouse. Absorption with 10 - 100 µg immunogen per ml diluted antiserum abolishes staining.

Recommended Positive Control:     Frozen sections of rat small intestine

Application                            Suitable for frozen tissue, paraffin sections after microwave treatment

                                               Immunohistochemistry, immunofluorescence microscopy

Antigen Recognized in

species tested so far           Rat, mouse

Reconstitution                    Dissolve in 50 - 100 µl distilled water, and dilute further with 0.1M PBS with

                                           1% BSA and 0.1% NaN3

Working Dilution                1:5000 - 1:1000 using FITC

Incubation Time                  Overnight at 4°C

Storage                                At 2-8°C (undiluted) or at -20°C (aliquots)

Quantity                               50 µl (lyoph.)

For Research Use Only


Guinea Pig polyclonal antibody to Vesicular acetylcholine Transporter (VAChT)

Cat#RDI-PRO16084              $562.00/vial

Category                                   Guinea pig polyclonal

Purification/Form                     Guinea pig serum

Immunogen                                Synthetic peptide from the C-terminus of rat VAChT conjugated to BSA

Description/Specificity            The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylchiolinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of rat and mouse. Absorption with 10 - 100 µg immunogen per ml diluted antiserum abolishes staining.

Recommended positive control for Frozen sections:     rat small intestine

Application Suitable for          frozen sections

                                                 Immunohistochemistry

Antigen Recognized in              Rat, mouse

Species (tested so far)

Reconstitution                          Dissolve in 50 - 100 µl distilled water, and dilute further with 0.1M PBS

                                                 with 1% BSA and 0.1% NaN3

Working Dilution                     1:5000 - 1:1000 using FITC

Incubation Time                       Overnight at 4°C

Storage                                     At 2-8°C (undiluted) or at -20°C (aliquots)

Quantity                                    50 µl (lyoph.)

For Research Use Only


-need a secondary antibody?
RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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