rev:       January 5, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Uncoupling Protein Antibodies (UCP1, UCP2 ,UCP3, UCP4 & UCP5)
-background:The regulation of body weight depends upon the calories intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (BAT and WAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolyals. BAT is able to dissipate energy as host via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domain (ETPDs). Both amino and C-terminal are predicted to be cytoplasmic. UCP1 is a member of the mitochondrial carrier protein family, which includes the ATP/ADP translocator, the phosphate, the 2-oxaglutarate/malate and the citrate (tricarboxylate) carriers. UCP1 plays an important role in thermoregulation in hibernating animals, neonates, and it contributes to rodent cold-and diet induced thermogenesis.

Because of the restricted expression of UCP1 and the lack of BAT in adult animals and humans. UCP1 may not be important in body weight regulation/energy expenditure in adults.Recently a novel UCP2, a homologue of UCP1, has been identified. In contrast with UCP1, UCP2 in widely expressed in adult human tissues and it is unregulated in WAT in response to fat intake. UCP2 is 59% homologous with UCP1. In the WAT of ob/ob and db/db mice, UCP2 expression is greatly elevated when compared to lean animals. Therefore, UCP2 has been implicated in the regulation of energy balance, body weight, and thermoregulation. Skeletal muscle contributes up to 40% of whole body epinephrine-induced thermogenesis in humans. Very recently, another homologue of UCPs,UCP3,has been cloned from human skeletal muscle. Human UCP3 protein (312 AA) has 71% and 57% homology with UCP2 and UCP1, respectively. The long (309 aa) and mature form of UCP3 is designated as UCP3L;UCP3s lacks the 6th potential TM domain and the PNBD domain implicated in the control of coupling efficiency of UCP1. Both forms of UCP3 are expressed in muscle. UCP3 expression is highly specific for skeletal muscle and it is unaffected by cold acclimation. It is also expressed in BAT of animals. UCP3 could play a critical role in energy expenditure and its regulation. Human UCP4 (323 aa; chromosome 6p11.2-q12) is expressed in both fetal and adult brain tissues. It is ~32% related to mammalian UCP1-3 and plant/nematode UCPs (~38-46% identity).

Human UCP4 (323 aa; chromosome 6p11.2-q12) is expressed in both fetal and adult brain tissues. It is ~32% related to mammalian UCP1-3 and plant/nematode UCPs (~38-46% identity). It is primarily expressed in the brain. Over expression of UCP4 in HEK cells reduces brain mitochondrial membrane potential. UCP5 or Brain Mitochondrial Carrier Protein 1 or BMCP1 (human 322 aa, chromosome Xq24, ~35% identity with UCP1-3) is also mainly expressed in the brain.


The following specific polyclonal rabbit antibodies to UCP1, UCP2, UCP3 ,UCP4 and UCP 5 using isoform-specific peptide sequences. These antibodies should prove to be useful in studying various UCPs. Species crossreactivity of UCPs antibodies and isoform is based only upon sequence homology information and it need to be experimentally confirmed. The control peptides are also available to determine specificity of antibodies.


Rabbit antiUncoupling Antibodies

Legend:M=Mouse; R=Rat; H=Human, Rb=Rabbit, D=Dog; P=Pig; C=Chicken;

--Antibodies are provided as affinity pure forms (1 mg/ml) preserved in 0.02% merthiolate.

--Control peptides are provided as 1 mg/ml solution (100 µg/100 µl) in PBS + 0.02% merthiolate. All products are for In vitro research use only. Not for human or drug use.


UCP1:primarily expressed in brown adipose tissues

see general refs: Feil S and Rafael J (1994) Eur J. Biochem 219, 681

                           Nature Genetics 15, 269-272

Rabbit anti-Murine UCP1     cat#RDI-MUCP12abrX     $531.00/100ug vial

-Antigen :a 12 amino acid peptide sequence (cytoplasmic) at the C terminus of the mouse/rat UCP1 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:100% mouse and rat (12/12aa), No significant homology with UCP2 or UCP3.

Antigen sequence homology: 12 amino acids

-rat (100%;12/12)

-hamster (91%, 11/12)

-rabbit/pig (83%, 10/12)

-human/dog (75%, 9/12)

-Use: WB (1-10ug/ml overnight at 4 DEg C with chemiluminescence detection), approx 32kDa in brown adipose tissue), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue).

-Control Peptide (100 µg): #RDI-MUCP12-CP $188.00

Rabbit anti-Murine UCP1      cat#RDI-MUCP19abrX     $531.00/100ug vial

-Antigen :a 19 amino acid peptide sequence (cytoplasmic) at the C terminus of the mouse/rat UCP1 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:100% mouse and rat (19/19aa), No significant homology with UCP2 or UCP3.

Antigen sequence homology: 19 amino acids

-rat (100%;19/19)

-hamster (94%, 18/19)

-pig 88%

-rabbit (78%, 16/19)

-human (78%, 16/19)

-canine (78%, 16/19)

-Use: WB (1-10ug/ml overnight at 4 DEg C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-MUCP19-CP $188.00


-UCP2:wide tissue distribution in mouse tissues including brain, kidney, liver, brown adipose tissue, heart and muscle)

-see general refs:   Nature Genetics 15, 269-272

                              Nature Genetics 15, 223-224

Rabbit anti-Murine UCP2      cat#RDI-MUCP2abrX    $531.00/100ug vial

-Antigen :a 14 amino acid peptide sequence near the C terminus of the mouse UCP2 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:100% mouse (14/14aa), 86% human (12/14aa). No significant homology with UCP1 or UCP3.

Antigen sequence homology: 14 amino acids

-rat, hamster 100%

-human,pig, bovine, canine (86%; 12/14)

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-MUCP2-CP  $188.00

Rabbit anti-Murine UCP2      cat#RDI-MUCP2CabrX     $531.00/100ug vial

-Antigen :a 14 amino acid peptide sequence at the C terminus of the mouse UCP2 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:100% mouse (14/14aa), 86% human (12/14aa). No significant homology with UCP1 or UCP3.

Antigen sequence homology: 14 amino acids

-rat, hamster (100%)

-human, pig, canine, bovine (86%; 12/14)

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-MUCP2C-CP $188.00

Rabbit anti-human UCP2    cat#RDI-UCP2MabrX     $531.00/100ug vial

-Antigen :an amino acid peptide sequence in the mid region of human UCP2 coupled to KLH

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection),

ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

Antigen sequence homology: 13 amino acids

-human/mouse/rat (100%; 13/13)

-canine/bovine/pig 92%

-Control Peptide (100 µg): #RDI-UCP2MC-CP $188.00


UCP3L:   see Boss, O et al (1997) FEBS Lett 408,38-42

                        Vidal-Plug A et al (1997) BBRC 235, 79-82

Rabbit anti-HUMAN UCP3      cat#RDI-UCP3abrX     $535.00/100ug vial

-Antigen :a 17 amino acid peptide sequence (transmembrane) of the human UCP3 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:100% human (17/17aa), 71% mouse (12/17aa). No signficant homology with UCP1 or UCP2.

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-UCP3-CP $188.00

Rabbit anti-HUMAN UCP3     cat#RDI-UCP3CabrX    $531.00/100ug vial

-Antigen :a 14 amino acid peptide sequence near carboxy terminus of human UCP3 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:100% human (14/14aa),

Antigen sequence homology: 14 amino acids

-dog, pig 100%

-mouse, rat, bovine 92%

No signficant homology with UCP1 or UCP2.

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-UCP3C-CP   $188.00

Rabbit anti-HUMAN UCP3     cat#RDI-UCP3MabrX    $531.00/100ug vial    HUMAN

-Antigen :an 18 amino acid peptide sequence located between the 3rd & 4th transmembrane domain of human UCP3 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:

Antigen sequence homology: 18 amino acids

-mouse (38%; 7/18)

-monkey 88%

-pig 83%

-bovine 61%

. Cross with mouse/rat may or may not occur. No signficant homology with UCP1 or UCP2.

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-UCP3C-CP $188.00


Rabbit anti-Rat UCP3      cat#RDI-RTUCP3MabrX    $531.00/100ug vial Rat/Mouse

-Antigen :an 18 amino acid peptide sequence between the 3rd and 4th transmembrane domain of rat UCP3 coupled to KLH

-Antibody Cross reactivity based on antigen sequence homology:100% rat (18/18aa), 100% mouse (18/14aa), only 38% human (7/18).

Antigen sequence homology: 18 amino acids

-mouse (100%; 18/18)

-hamster 94%

-canine 66%

-bovine 61%

-monkey 63%

-human (38%, 7/18)=reactivity not anticipated but   possible , see anti-human ab

No signficant homology with UCP1 or UCP2.

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-RTUCP3M-CP    $188.00


Rabbit anti-Uncoupling Protein-3   (Mouse, Rat/Human)

cat# RDI-MUCP3Xabr  $469.00/100ul

Package: 100 ug of affinity purified rabbit serum in 0.1ml (1mg/ml) with 0.05% sodium azide. Store at -20C. Avoid repeated freeze/thaw cycles.

Immunogen: Synthetic Peptide: C R(295) A L M K V Q V L R E S P F (308)

-immunizing peptide corresponds to amino acid residues 295-308 of the mouse and rat UCP-3 protein. This peptide differs from the porcine and human UCP-3 sequence by a single amino acid substitution. The immunizing peptide (Cat.RDI-MUCP3X-CP $188.00/50ug) is available for use in neutralization and control experiments.

Use: -western blot 1:1000 detects uncoupling protein-3 (UCP-3) from human, rat and mouse tissues. This antibody does not detect UCP-3 from mouse liver or kidney. This ab has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing UCP-3 from rat heart homogenate.

Background: The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP-1 is abundantly expressed in brown adipose tissue of mammals. UCP-1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP-2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP-2 to be expressed by a variety of tissues. UCP-3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.

For In Vitro Research Use Only


Rabbit anti-Human UCP4 cat#RDI-UCP4abrX    $535.00/100ug vial  Human

-Antigen :a 16 amino acid peptide sequence within the cytoplasmic amino terminus of human UCP4 coupled to KLH

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA,(potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)

-Control Peptide (100 µg): #RDI-UCP4-CP $188.00


Rabbit anti-RAT UCP4 c  at#RDI-RTUCP4abrX   $531.00/100ug vial  RAT/Mouse/Human

-Antigen :a 14 amino acid peptide sequence within the within the 1st extracellular region of rat UCP4 coupled to KLH

Rat UCP4 may be alternatively spliced to produced at least 3 transcripts with 365 aa (isoform C), 344 aa (isoform b), and 322 aa (isoform a). The rat control peptide sequence is 100% conserved in all UCP4 isoform. It is 85% conserved in human and 78% in mouse UCP4. It has no appreciable homology with UCP1-3/UCP5 or any other protein.

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)-Control Peptide (100 µg): #RDI-RTUCP4-CP $188.00


Rabbit anti-RAT UCP5 cat#RDI-RTUCP5abrX   $531.00/100ug vial    RAT/Mouse/Human

-Antigen :A 16 amino acid peptide sequence within the 1st extracellular region of rat UCP5 coupled to KLH

UCP5 may be alternatively spliced to produced at least 3 transcripts: UCP5L has a unique 22-aa unique N-terminal sequence; UCP5S is missing 3 aa (23-25 aa) and UCP5SI has 31 aa insertion between TM II-IV. The RTUCP5abrx sequence is conserved in all 3 isoforms in rat and mouse UCP5. It is 94% conserved in human UCP5/BMCP1. IT has no appreciable homology with UCP1-4.

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)-Control Peptide (100 µg): #RDI-RTUCP5-CP $188.00


Rabbit anti-Human UCP5   cat#RDI-UCP5abrX   $531.00/100ug vial   Human

-Antigen :A 16 amino acid peptide sequence within cytoplasmic N-terminus of human  UCP5 coupled to KLH

UCP5 may be alternatively spliced to produced at least 3 transcripts: UCP5L has a unique 22-aa unique N-terminal sequence; UCP5S is missing 3 aa (23-25 aa) and UCP5SI has 31 aa insertion between TM II-IV. The sequence is conserved in all 3 isoforms in rat and mouse UCP5. It is 100% conserved in rat and mouse UCP5/BMCP1. It has no appreciable homology with UCP1-4.

-Use: WB (1-10ug/ml overnight at 4 DEG C with chemiluminescence detection), ELISA, (potentially histochemistry at approx 10-20ug/ml on formaldehyde or 4% PFA fixed tissue)-Control Peptide (100 µg): #RDI-UCP5-CP $188.00

-copyright by owner


-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.
RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

EMAIL:margaret@cellular-research.com

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