rev:      December 13, 2004

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  ANTIBODIES  

                                                      (anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


TRK ANTISERA
Mouse anti-TRK-A (extracellular-human)

cat# RDI-TRKAabmX      $344.00/50ug

       RDI-TRKAabmX1    $562.00/100ug

Presentation: 50ug (0.050ml) or 100ug (0.1ml) at 1mg/ml in PBS (0.2 micron filtered)

Purification: Protein G of ascites

immunogen: recombinant human TrkA extracellular domain

isotype: mouse IgG2B

reactivity: human (not tested for others)

Use: -Western blot; 1–2ug/ml to detect approx 5ng/lane recombinant human TRK-A (non-reducing) or 25ng/lane (reducing) condition

-Elisa: in direct Elisa (no cross observed with recombinant human TrkB, rec human TRKC or rec rat TRKC)

Storgage: Antibody can be stored at 2 ° -8 ° C for 1 month without detectable loss of activity. Antibody can also be aliquoted and stored frozen at -20 ° C to -70 ° C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

For In Vitro Research Use Only


Rabbit anti-TRK-A (Human/Rat/Mouse)

cat#RDI-TRKAabrX   $562.00/200ug in Tris-glycine buffer

-protein A purified rabbit polyclonal

-immunogen: recombinant extracellular region of rat TRKA

-reacts with human, rat and mouse TRKA, no cross observed with TrkB or TRKC

-use: western blot, approx 1-2ug/ml (pos controls include EGF stimulated human A431, mouse 3T3 and rat PC-12 cells), immunoprecipitaion, and histochemistry


Rabbit anti-TRK-B (Rat/Mouse)

cat#RDI-RTTRKBabrX   $562.00/200ug in Tris-glycine buffer

-protein A purified rabbit polyclonal

-immunogen: extracellular region of rat TRKB aa 1-429

-reacts with rat and mouse TRKB at 145Kda

-use: western blot, approx 1-2ug/ml (pos controls include mouse membrane microsomal preparations


Goat anti-TRK-C (Human/Rat/Mouse)

cat#RDI-TRKCabGX     $562.00/200ug in Tris-glycine buffer

-protein A purified Goat polyclonal

-immunogen: extracellular region of rat TRKA (aa 1-429)

-reacts with human, rat and mouse TRK-C at 145kda

-use: western blot, approx 1-2ug/ml (pos controls include mouse membrane microsomal preparations


Chicken anti-TRK-C (Human and Rat)

cat#RDI-TRKCabCK   $562.00/200ug in Tris-glycine buffer

-PEG/ammonium sulphate purified chicken polyclonal

-immunogen: fusion protein corresponidng to aa 57-243 of human TrkC

-reacts with human and rat TRK-C at 145kda

-use: western blot, approx 1-2ug/ml (pos controls include HS294T RIPA lysate)


RABBIT POLYCLONAL  ANTIBODY TO: HUMAN trk ONCOPROTEIN

PRODUCT CODE          RDI-CBL770       $375.00

CLONE                           Polyclonal

ISOTYPE                        IgG

IMMUNIZING ANTIGEN Synthetic peptide: (Cys)-Ala-Leu-Ala-Gln-Ala-Pro-Pro-Val-Tyr-Leu-Asp-Val-Leu-Gly selected from the C-terminal catalytic domain of the human trk proto-oncogene amino acid sequence. This sequence is also present at the C-terminus of the trk oncogene amino acid sequence. Four amino acid changes exist in the aligned region of the trkB sequence, five amino acid changes exist in the aligned region of the trkC sequence.

SOURCE                       Rabbit serum

PURIFICATION METHOD Protein A affinity chromatography

SPECIFICITY CBL 770 detects a band at 140 kDa on immunoblots of E25-427 a transfected cell line that expresses the trk proto-oncogene product. A band at 110 kDa was also detected. Prior incubation of the antibody with peptide blocks these bands.CBL 770 detects a band at 70 kDa on immunoblots of E106-632 a transfected cell line that expresses the trk oncogene product. Prior incubation of the antibody with peptide blocks these bands. CBL 770 35S immunoprecipitates a 140 kDa protein from lysates of methionine labelled PC12 cells.

APPLICATIONS  *Immunoblotting*Immunoprecipitation

REFERENCES Martin-Zanca, D. et al. Mol. & Cell. Biol. 1, 24-33 (1989)

LOT SPECIFIC DATA

PRESENTATION The purified immunoglobulin is presented at a concentration of 100µg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedure


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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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