rev:      January 2, 2005

HOME (index page)

Return (alphabetical antibody page)

Return  (anti-enzyme antibodies..index)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


-see also new human and rabbit GAPDH below
Monoclonal mouse anti-rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH)


CAT#: RDI-TRK5G4-6C5          lot#02/10-G4-C5

PRICE: $375.00/mg  or  $344.00/mg for 2-9   $312/mg 10+

Clone: 6C5   This clone has been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with rabbit muscle GAPDH.

Reactivity: MoAb 6C5 reacts with GAPDH from fish,frog, chicken, rabbit, mouse and human skeletal muscle. -tested positive by western blot on fish, frog, chicken, mouse, rabbit and human skeletal muscle, and mouse , canine, porcine and human cardiac tissue extracts. Negative on yeast, goat cardiac and bovine cardiac. Others not tested.

Mouse isotype: IgG1 for clone 6C5

Application: Detection of GAPDH. Immunoaffinity purification of GAPDH. MoAb 6C5 works in ELISA and Western blotting (detects band at approx 36kDa on both human erythrocyte membranes and rabbit muscle, approx 1ug/ml with chemiluminescence detection) Specific antibody titer in indirect ELISA is 10-6.

-WB:start at 1ug/ml with chemiluminescence detection

-Immunoprecipitation.

Reference: FEBS letters, volume 300, number 1, 9-12, 1992.

Presentation: Each vial contains 1mg of antibodies (concentration 5.65 mg/ml), in 0.180ml PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG C. Avoid repeated warming cycles.

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. During the last decade, many findings have been made concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases such as Alzheimer’s and Huntington’s. GAPDH is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types.

GAPDH molecule is composed of four 36 kDa subunits. The molecular weight for GAPDH is 146 kDa. Since it is expressed in all cells, it is becoming the marker of choice as a loading control in such procedures as Western Blotting. see also our pan actin (cat#RDI-ACTINabm-N5 $438.00/100ug) for use as loading controls.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

FOR IN VITRO RESEARCH USE ONLY

NOTE:Before Opening., Make sure Cap is tight and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in bottom of vial. If desired, can rinse into a larger vial and add 0.820ml PBS with 0.1% NaN3 to yield a 1mg/ml stock.


Human GAPDH (Glyceraldehyde-3-phosphate dehydrogenase)

cat#RDI-TRK8G4      $315.00/100ug

       RDI-TRK8G4X  $875.00/0.5mg

Presentation: suspension in 5mM HEPES, 5mM EDTA, 1mM DTT pH8.5, 80% ammonium sulphate

-Source: Human heart tissue. Blood sample from the tissue donors was tetsted and found neggative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, syphilis

-Purity: >98% by SDS-PAGE

-Store 4-8 DEg C (avoid multiple freeze-thaw cycles)

-For In Vitro Research Use Only-not for use in or on humans or animals


Rabbit GAPDH (Glyceraldehyde-3-phosphate dehydrogenase)

cat#RDI-TRK8G4R      $315.00/100ug

       RDI-TRK8G4RX  $875.00/0.5mg

Presentation: suspension in 5mM HEPES, 5mM EDTA, 1mM DTT pH8.5, 80% ammonium sulphate

-Purity: >98% by SDS-PAGE

-Store 4-8 DEg C (avoid multiple freeze-thaw cycles)

-For In Vitro Research Use Only-not for use in or on humans or animals


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return (alphabetical antibody page)

Return  (anti-enzyme antibodies..index)

Ordering terms