rev:June 18, 2003

HOME (index page)

Return (alphabetical antibody page)

Return  (anti-enzyme antibodies..index)


  ANTIBODIES  

(anti-Human and others as indicated)

Research Diagnostics Inc  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Caldesmon (see 2 monoclonals below)
Monoclonal mouse anti-duck gizzard caldesmon

CAT#: RDI-TRK5C5-12B5

PRICE: $300.00/mg


Clone: 12B5

This clone has been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with duck gizzard caldesmon (heavy chain).

Rectivity: MoAb 12B5 react with heavy and light forms of human, duck and chicken caldesmon. MoAb 12B5 do not cross-react with other proteins. Kd=10-12M.

Mouse isotype: IgG1

Application: Detection of caldesmon. Immunoaffinity purification of caldesmon. MoAb 12B5 work in ELISA and immunoblotting.

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains 1 ml of antibodies with concentration 1 mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at +4C.


Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care muse be taken when handling this product.


FOR IN VITRO RESEARCH USE ONLY


Mouse anti-L-Caldesmon Monoclonal ab

cat# RDI-CALDESMabm    $350.00/vial 50ug

       RDI-CALDESMabm-1  $600.00/vial 150ug

Background: Caldesmon is a protein component of the thin filaments of smooth muscle myofibrils.It is also localized in the stress fibers of fibroblasts.Caldesmon was identified as a Ca2+ /Calmodulin-binding protein with molecular weight of 120-150 kDa (H-Caldesmon)and 70-80 kDa (L-Caldesmon).H-Caldesmon is the primary isoform in smooth muscle while L-Caldesmon is most abundant in non-muscle cells.Caldesmon is capable of binding two calmodulin molecules,one at either end of the protein.Additionally,Caldesmon s an actin,myosin,and tropomyosin-binding protein.Human L-Caldesmon is a protein of 538 amino acids with mobility of 80 kDa.In vitro L-Caldesmon inhibits the actomyosin ATPase n an F-Actin-dependent manner.L-Caldesmon may play an important function in motile processes such as secretion and organelle movement.

Antigen: derived from human aa 251-385

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) purified antibody (0.25mg/ml) in a 50%glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

Use: Suitable for western blot on human, rat and mouse using approx 0.1-0.2ug/ml ab on total cell lysate. Major band at Mw 80kDa. Recommend 2-3 hour RT or overnight 4 DEG C incubation with enhanced chemiluminescence detection. Also for immunofluorescence (not recommended for IP or histochemistry)

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner

Positive western blotting Control:

Hs68 Fibroblast Lysate was derived from human foreskin.

ell lysates available on request

see     http://www.researchd.com/controls/lysates.htm


ANTI-CALDESMON MONOCLONAL ANTIBODY

PRODUCT CODE :  RDI-CALDESMONabmX     $250.00/vial 100ul

CLONE: TD107

ISOTYPE: IgG1

SOURCE: Tissue culture supernatent or ascites

PURIFICATION METHOD: none

SPECIFICITY: The antibody immunoblots with the caldesmon band (80kDa) on SDS/PAGE gels from platelets and the 140kDa isoform of smooth muscle. Immunofluorescence staining on methanol fixed fibroblasts shows strong stress fibre reactivity.

reactivity: human, mamalian, yeast and avian

APPLICATIONS: * Immunofluorescence staining on cryostat sections.

                              * Suitable for immunoblotting.

We recommend that each laboratory determine an optimum working titre for use in its particular application.

Precautions: For In vitro research Use Only. Not  for use in or on humans or animals or   for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Research Diagnostics Inc

Pleasant Hill Road

Flanders NJ 07836

phone (800) 631-9384 or (973) 584-7093

fax     (973) 584-0210

EMAIL:ResearchD@aol.com

Return  (anti-enzyme antibodies..index)

Return (alphabetical antibody page)

Ordering terms