rev: April 6, 2005
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(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see additional info at: http://www.researchd.com/humanag/pappainfo.pdf
$438.00/1mg $406.00/mg 2-9 $375.00/mg 10+ Bulk on Request
catalog# RDI-TRK4P41- clone# example: RDI-TRK4P41-5H9 (for clone 5H9)
Monoclonal antibody: 5H9, 10E1, 10E2, 4G11, 10A5, 3C8, 10H9, 18A10, 11E4
Hybridoma clones, producing MAbs, have been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A antigen.
Specificity: Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of two PAPP-A subunits and two pro-MBP subunits) Clone 11E4 is specific to proMBP subunit.
Mouse isotype: IgG2b for MAb 5H9, 10E1 and 10E2
IgG2a for clones 4G11, 10A5, 3C8, 10H9 and 18A10
Application: Detection of human PAPP-A antigen in immunoassay.
Recommended pairs (coating - conjugate) : for the detection of PAPP-A in blood of AMI patients and patients with unstable angina (capture - detection): 4G11 3C8, 4G11 10H9, 10E2 10E1
Best pairs for the detection of PAPP-A in pregnancy serum (capture detection): 4G11 3C8, 4G11 10H9, 10A5 3C8, 10E2 10E1, 10E2 5H9, 5H9 10E2
MAbs 4G11, 10A5, 18A10 (best) and 10E1 recognize PAPP-A subunit in the heterotetrameric complex in Western Blotting. MAb 11E4 recognizes proMBP subunit in Western Blotting
Purification: All MAbs are purified by chromatography on protein A Sepharose. Clone 10E2 is additionally purified by gel-filtration. Purity of all MAbs is tested by electrophoresis.
Presentation: Each vial contains antibodies in PBS, pH 7,4, containing 0,1% of sodium azide as preservative. Store at + 4 °C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
For Research Use only
FOR IN VITRO RESEARCH USE ONLY
CAT#: RDI-TRK8P64 $750.00/100ug $1500.00/0.5mg $2500.00/1mg
Package: lyophilized protein from PBS containing 3% dextran as stabilizer
Source: Pooled retroplacental blood Starting material was tested and found negative for HBs/Ag,HCV and HIV-1 and 2 antibody, syphilis.
Purity: >85% (SDS-PAGE)
Immunological activity: Was tested in immunofluorescent assay.
Storage: +4C. The product is stable at least one year.
Purification: Gel-filtration and ion-exchange chromatography.
Application: PAPP-A can be used for in vitro testing of Down syndrome and other fetal disorders. Reference: Human Reproduction, 1995,V10,No.2,pp453 -458.
For In Vitro or Further Manufacturing Use Only
RDI Division of Fitzgerald Industries Intl
(Research Diagnostics Inc)
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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