rev: May 14, 2008

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see monoclonals and polyclonals below


PRODUCT DATA SHEET


Monoclonal mouse anti-horseradish peroxidase


CAT#: RDI-TRK4P14-2H11, new cat#: 10R-P156a

PRICE: $412.50/1mg

Clone: clone3P/2H11

This clone has been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified peroxidase from horseradish.

Specificity: Horseradish peroxidase, isoforms with pl from 3.5   to 9.5

Reactivity: Specific antibody titer in indirect ELISA is 1/100 000. MoAb 3P/2H11 do not influence enzymatic activity upon immune complex formation.

Mouse isotype: IgG2b

Application: Peroxidase-antiperoxidase complex for immunohistochemistry and ELISA.

Purification: Globulin fraction from ascitic fluid reprecipitation in semisaturated ammonium sulfate.

Presentation: Each vial contains 1 ml of antibodies with concentration 1 mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at +4C.


Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care muse be taken when handling this product.


FOR IN VITRO RESEARCH USE ONLY


also available:Goat, sheep and rabbit anti-HRP

cat#RDI-HRPabG, new cat#: 20R-PG005   $375.00/50mg

     #RDI-HRPabS, new cat#: 20R-PS001   $375.00/50mg

     #RDI-HRPabr, new cat#: 20R-PR040    $375.00/50mg

DATA SHEET: Rabbit anti-Horseradish Peroxidase

Catalog#: RDI-HRPabR $250.00/vial

Size: 50 mg (lyophilized)

Antibody Concentration: 10mg/ml (by UV at 280nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: none

Antigen: horseradish peroxidase

Purification: IgG fraction isolated by delipidation, salt fractionation and ion exchange chromatography with dialysis against above buffer system.

Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher. approx 1:15K+ in ELISA

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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