rev: January 7, 2004
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(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Monoclonal mouse anti-neuron specific enolase
CAT#: RDI-TRK4N6-5G10, or RDI-TRK4N6-5E2,or
RDI-TRK4N6-5A4, or RDI-TRK4N6-1C1
PRICE: $375.00/mg or $344.00/mg 2 or more (any combination) Bulk on request
Clone: 5G10, 5E2, 5A4, 1C1
These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified neuron-specific enolase from human brain.
Specificity: Neuron-specific enolase (NSE), human
Reactivity: MoAb react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
Specific antibody titer in indirect ELISA is 1/1, 000.000 for all clones. MoAb 5G10, 5A4 and 5E2 may be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections (recommend clone 5G10). Recommended dilution is 1:100=1:300. Optimal dilution must be determined by experimentation.
All antibodies work in ELISA.
The following pairs of our anti-NSE clones can be used to make an ELISA: 1C1 - 5A4, 1C1 - 5E2 and 1C1 - 5G10. According our data best results were obtained with MAb 5G10 direct coating and MAb 1C1-HRP conjugate. Sensitivity approx 2-5ng/ml
(clone 1C1 can be purchased HRP conjugated, cat#RDI-TRK4N6-1CHRP $688.00/1mg, $625.00/mg 2+)
Mouse isotype: Clones 5E2, 1C1, 5A4 - IgG2a
clone 5G10 - IgG2b
Application: Detection of NSE in ELISA and immunohistochemistry.
Presentation:Each vial contains 1 ml of antibodies with concentration 1 mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store 4-8 DEG C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
FOR IN VITRO RESEARCH USE ONLY
-results within: 2hours 1 step procedure
- detection limit: <1ug/liter
-measuring range: 1-150ug/liter
-sample volume: 25ul
-hook effect: no hook up to 15,000 ug/lier
-stability: up to 12 months from date of production
-standard range: 6-165 ug/liter
-incubation temp: 20-28 DEG C
detection: 620nm or 405nm
refernece value: <13ug/ml
CAT#: RDI-TRK8NS3 $750.00/100ug
Source: Postmortal human brain
Source: Postmortal human brain Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies and syphilis
Purity: More than 95% (SDS-PAGE).
Purification: Purified by ion-exchange and gel-filtration chromatography according to Pahlman S. et al "Tumor Biology:, 1984, v.5, pp 127-139.
Activity: Specific activity-985 umol/min mg at 25 DEG C determined in 45mM imidazole, pH 6.8, 45mM Kcl, 1.33mM Mg2SO4, 1mM 2-PG. One unit is defined as the amount of NSE concentrated 1 mol of 2-phospho-D-glycerate pe rmin at 25 DEG C(J. Biochem 87, 1587-1594, 1980) Subunit composition is gamma-gamma.
Immunological Activity: Was tested in Elisa and immunoblotting with anti-NSE antibodies
Presentation: Solution in 0.1M Tris-Hcl, 5mM Mg2SO4, 0.25M KCL, pH 5.1 adjusted with sodium phosphate. no preservative added.
Store -20 DEG C. Avoid repeated freeze-thaw cycles.
Protein Concentration: see actual batch data (approx 0.85mg/ml according to spectrophotometer using E280nm
For Research Use Only
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. W are not responsible for any patent infringements that might result with the use of or derivation of this product
Catalog #RDI-TRK8NS4 $750.00/100ug $1125.00/500ug $1500.00/1mg
Source: Isolated from frozen human brain: Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, syphilis.
Purity: > 95 % according to 10 % SDS-PAGE
Purification: Purified by ion-exchange chromatography, gel-filtration and ammonium sulphate fractionation
Activity: Determined in 45 mM imidasole pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4, 1 mM 2-PG.
Immunoreactivity: Does not react with anti-NSE in ELISA and WB
Presentation : Solution in 10 mM TRIS/HCL, 5 mM MgSO4, pH 7.5
Protein concentration : Determined by BCA method ("Pierce" USA)
Storage: - 20 °C
Application: This product is for in vitro research use or further manufacture.Not for use in or on humans or animals-not for use in diagnsotics
San Jose, 95123 CA Snell ave 658
or (800) 370-2222
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