rev:       April 12, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


THYROGLOBULIN
PRODUCT DATA SHEET


Monoclonal mouse anti-human thyroglobulin

CAT#: RDI-TRK2TG12-5E6

            RDI-TRK2TG12-5F9

           RDI-TRK2TG12-12G9

PRICE: $375.00/mg or $344.00/mg for 2 or more   Significant Bulk Discounts

Clone: 24Tg/5E6, 24Tg/5F9, or 12G9

These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with thyroglobulin from human thyroid gland.

Reactivity: MoAb specifically recognize human thyroglobulin. Specific antibody titer in indirect ELISA is 1/100 000. Clone 5F9 also reacts with bovine thyroglobulin., Clone 12G9 cross reacts with bovine, pig, cat, rat dog and mouse thyroglobulins.

Mouse isotype: 5E6, 5F9 =IgG2b  

                         clone 12G9=IgM

Application: Detection of human thyroglobulin in two-site enzyme immunoassay. We recommend to use MoAb 24Tg/5F9 for coating and MoAb 5E6 for labeling.

Purification: Protein G Sepharose

Presentation: Each vial contains 1.0mg in 0.2ml (5mg/ml) PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG C.

Material safety note:

This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be  followed when handling this material. Contains sodium azide (0 .1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when  handling this product.

FOR IN VITRO RESEARCH USE ONLY

see thyroglobulin antigen at:  http://www.researchd.com/humanag/humags.htm


Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

FOR IN VITRO RESEARCH USE ONLY


Polyclonal Antibody to Thyroglobulin

Cat#RDI-PRO10855    $312.00

Category                        Rabbit polyclonal

Purification/Form          Rabbit serum

Immunogen                     Isolated from human thyroid

Description/Specificity

                                     Specifically identifies follicular epithelium and colloid of the thyroid. It can, therefore, be applied for the classification of thyroid neoplasms, especially the differentiation between follicular and papillary carcinoma (TH-positive) and medullary carcinoma (TH-negative). Undifferentiated carcinomas will not react with the antibody. Furthermore, the antibody can be used for the characterization of metastases of unknown primary tumors. The antibody reacts exclusively with follicle epithelium and colloid in the thyroid. Antigen localization: membrane, cytoplasm

Recommended Pos Control: Thyroid tissue

Application                           Immunoblotting

                                              Immunohistochemistry

                          Suitable for frozen and paraffin sections

                          Proteolytic treatment not required

Antigen Recognized            Human

in Species (tested so far)

Working Dilution               1:50 - 1:100

Dilution Buffer                  PBS with 1% BSA and 0.1% Na-Azide

Incubation Time                 1 h at room temperature

Storage                               At 2-8°C (undiluted) or at -20°C (aliquots)

Volume                               0.25 ml

For In Vitro research Use Only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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