rev:    December 16, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see below monoclonal and 2 polyclonals

PRODUCT DATA SHEET

Monoclonal mouse anti-human growth hormone  (see also recombinant GH)

CAT#:

RDI-TRK2G2-Gh29 (clone Gh29)

RDI-TRK2G2-GHG2 (clone GhG2)

RDI-TRK2G2-GHB9 (clone GhB9)

PRICE: $375.00/mg or  $344.00/mg 2-9  $312/mg 10+

Clone: Gh29, GhG2, GhB9

These clone has been derived from hybridization of   X63-Ag8-653 myeloma cells with spleen cells of a   Balb/c mice immunized with purified recombinant  human growth hormone (hGH).

Specificity: growth hormone, human

Reactivity: MoAb Gh 29 react with recombinant and natural human growth hormone with Kd=10-9M. There is no cross-rectivity with HPRL, FSH, LH and insulin. Specific antibody titer is 1:1 000,000 in indirect ELISA.

Recommended pair for sandwich ELISA:   (capture conjugate):   GhG2- GhB9

(conjugate must be labeled in your own lab or custom conjugated -inquire) 

Mouse isotype: IgG1

Application: Detection of human growth hormone.

Purification: Purified by chromatography on protein A Sepharose.

Purity is tested by electrophoresis.

Presentation: Each vial contains 1.0mg of antibodies in 0.1ml(10.2 mg/ml) PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG C.

Material safety

note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

FOR IN VITRO RESEARCH USE ONLY

NOTE:Before Opening., Make sure Cap is tight and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in bottom of vial. If desired, add 0.9ml PBS buffer with 0.1% NaN3 to bring up to 1mg/ml.


DATA SHEET :  Rabbit anti-Human Growth Hormone (Frozen & Parrafin   Sections)

cat# RDI-HGHabr   $375.00/vial


Specificity: Human Growth Hormone in normal pituitary gland and in pituitary adenomas associated with arcomegaly,

Preparation: Lyophilised rabbit serum containing 15mM sodium azide. Reconstitute with 0.25ml of sterile distilled water.

USE: Effective on paraffin wax embedded tissue and on frozen sections.

Protocol: Immunohistochemistry:Typical working dilution 1:50 - 1:100. 30 Minutes incubation at 25'C. Standard ABC technique.

Western blotting: not checked

Positive controls: pituitary gland

Staining pattern: cytoplasmic

Storage and stability: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, aliquot in non frost freze freezer, Avoid repeated freeze thaw cycles. Prepare fresh working dilutions daily.

Stricly for in vitro research use only-Not for use in or on humans or animals-not for use in diagnostics


RABBIT Anti- human Growth Hormone HGH ANTIBODY

cat# RDI-HGHabrX1   Price: $375.00/1ml

lot#

Form: neat sera (no preservative)

Presentation: 1ml neat rabbit sera

Titer: in 125I assay, 1ml=150k T

Specificity: HGH 100%

hFSH 0.2%

hLH 0.2%

hTSH 0.2%

hPrl 0.3%

Storage: -Refrigerate at 2 to 8 DEG C for immediate use -Store at -20 DEG C for long term -Avoid frequent freeze thaw cycles -Recommend adding preservative of choice (as 0.1% sodium azide or 0.01% merthiolate).

FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

**All characteristics and suitability of use must be determined by the end user. Changes in assay design will greatly effect all characteristics. RDI assumes no responsibility or liability for bulk antisera. No warranty either expressed or implied is available for this product.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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