rev:   October 18, 2001

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


TIE-1 & TIE-2 Cell Membrane Receptors

see also new recombinat sTIE-1/FC chimera and recombinant sTIE-2/Fc chimera below


rabbit anti-Tie-1 (Human/Mouse/Rat)

cat#RDI-TIE1Cabrx    $531.00/100ug antigen aff pure

-immunogen:20aa mapping in carboxy terminal region of human (identical to corresponding mouse,rat and bovine sequence)

-reactivity: human, mouse and rat

-suitable for western blotting, NIH/3T3 cells can be used as positive control. Tie-1 ~135-140 kDa.(other applications not tested) -blocking pepide cat#RDI-TIE1CX-CP $188.00/100ug


rabbit anti-Tie-1 (Human/Mouse/Rat)

cat#RDI-TIE1Nabrx   $531.00/100ug antigen aff pure

-immunogen:21aa mapping in the amino terminal region of human Tie-1

-sequence homology:(mouse 90%, 19/21 aa) and bovine (63%, 12/19 aa) Tie-1

-reactivity: human, mouse and rat

-suitable for western blotting,NIH/3T3 cells can be used as positive control. Tie-1 ~135-140 kDa.(other applications not tested)  -blocking pepide cat#RDI-TIE1NX-CP $188.00/100ug


rabbit anti-Tie-2 (Human/Mouse/Rat)

cat#RDI-TIE2Cabrx   $531.00/100ug antigen aff pure

-immunogen:20aa mapping in carboxy terminal region of human

-reactivity: human, mouse and rat (identical to corresponding mouse,rat and bovine sequence)

-suitable for western blotting,NIH/3T3 cells can be used as positive control. Tie-2 is ~140 kDa (other applications not tested)

-blocking pepide cat#RDI-TIE2CX-CP $188.00/100ug


rabbit anti-Tie-2 (Human/Mouse/Rat)

cat#RDI-TIE2Nabrx    $531.00/100ug antigen aff pure

-immunogen:18aa mapping in amino terminal region of human

-reactivity: human, mouse and rat (identical to corresponding mouse and rat sequence, approx 90% homology with bovine)

-suitable for western blotting, NIH/3T3 cells can be used as positive control. Tie-2 is ~140 kDa (other applications not tested)

-blocking pepide cat#RDI-TIE2NX-CP $188.00/100ug

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Mice lacking TIE 1 or TIE 2 are lethal. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis. TIEs appear to be acting downstream of the VEGFRs. Tie-1 (human 1138 AA; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.

For Research Use Only


Recombinant Human sTIE-1/Fc Chimera

Description: Recombinant human soluble TIE-1 was fused with the Fc part of human IgG1. The recombinant mature sTIE-1/Fc is a disulfide-linked homodimeric protein. The sTIE-1/Fc monomers have a mass of approximately 125 kDa. The soluble receptor protein consists of the full extracellular domain (Met1-Glu749). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-1 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-1 display similar angiogenic defects. The recombinant mature TIE-1/ Fc is a disulfide-linked homodimeric protein. Human TIE-1/Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.

Source:                       Insect cells

Molecular Weight:     250 kDa

Subunit:                     glycosylated dimer

Purity:                       > 90%, by SDS-PAGE and visualised by silver stain

Endotoxin level:       < 0.1 ng per mg sTIE-1/Fc

Stabilizer:                 none

Buffer:                       PBS

Formulation:              lyophilized

Biological Activity: Since a ligand for TIE-1 has not yet been identified, the recombinant protein was not tested for biological activity.

Reconstitution: The lyophilised sTIE-1/Fc is soluble in water and most aqueous buffers. The lyophilised sTIE-1/ Fc should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six months at –20°C to –70°C. Reconstituted sTIE-1/Fc should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: sTIE-1/Fc is offered for research use. Not for drug use. Not for human use!

Catalogue number: RDI-SFC-011 Size: 100 µg   $438.00   $375.00/vial 5+  Bulk quotes on request

Range: 10-100 ng/ml

Literature: [Sato et al., PNAS 90:9355, 1993; Gale et al., Gen Dev 13:1055, 1999]


Recombinant Human sTIE-2/Fc Chimera

Description: Recombinant human soluble TIE-2/Tek was fused with the Fc part of human IgG1. The recombinant mature sTIE-2/Fc is a disulfide-linked homodimeric protein. The sTIE-2/Fc monomers have a mass of approximately 125 kDa. The soluble receptor protein consists of the full extracellular domain (Met1-Val730). TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or TIE-2 display similar angiogenic defects. The recombinant mature TIE-2-Fc is a disulfide-linked homodimeric protein. Human TIE-2-Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions.

Source: Insect cells

Molecular Weight: 250 kDa

Subunit: glycosylated dimer

Purity: > 90%, by SDS-PAGE and visualised by silver stain

Endotoxin level: < 0.1 ng per mg sTIE-2/Fc

Stabilizer: none

Buffer: PBS

Formulation: lyophilized

Biological Activity: Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4 µg/mL (100µl/well), it binds rh Angiopoietin-2 with a linear range of 2 - 100 ng/ml.

Reconstitution: The lyophilised sTIE-2/ Fc is soluble in water and most aqueous buffers. The lyophilised sTIE-2/Fc should be reconstituted in PBS or medium to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six months at –20°C to –70°C. Reconstituted sTIE-2/

Fc should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: sTIE-2/Fc is offered for research use. Not for drug use. Not for human use!

Catalogue number: RDI-SFC-013    Size: 100 µg  $438.00   $375.00/vial 5+  Bulk quotes on request

Range: 10-100 ng/ml

Literature: [Sato et al., PNAS 90:9355, 1993; Gale et al., Gen Dev 13:1055, 1999]


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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