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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


THYROID HORMONE RECEPTOR ANTIBODIES

Mouse anti-Thyroid Hormone Receptor Monoclonal ab


cat# RDI-THYHR-C3    $406.00/vial


Background: This antibody recognizes the TRB-1 and also cross reacts with the TRalpha-1 isoforms of thyroid hormone receptor (TR) in human samples.


isotype: mIgG1

clone: C3

package: 100ul of diluted ascities fluid with 0.05% sodium azide added

antigen: produced from a fusion of P3x63 Ag8653 myeloma cells and splenocytes from a Balb/c mice immunized with purified truncated fragment of human TRB-1 (Lys201-Asp456 over-expressed in E. Coli)


Specificity: epitope mapping in hormone binding domain of hTRB- 1 at amino acids 235-414 which is conserved in hTRA-1.


Use: -immunoprecipitation: immunoprecipiates 52kDa and 55kDa proteins, representing the products of two alternate start sites of TRB-1 and a 48 kDa protein representing TRA-1.
       -western blot:detects recombinant TRB-1 which was expressed in E. Coli
       -gel shift assay


Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


References: -M.K. Bhat, et al. BBRC, 195:385-392, 1993


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-Thyroid Hormone Receptor B-1 Monoclonal ab

cat# RDI-THYHRB1-J52    $406.00/vial


Background: This antibody recognizes the TRB-1 isoforms of thyroid hormone receptor (TR) in human and rat samples.


isotype: mIgG1

clone: J52

package: 100ul of diluted ascities fluid with 0.05% sodium azide added


antigen: produced from a fusion of P3x63 Ag8653 myeloma cells and splenocytes from a Balb/c mice immunized with purified truncated fragment of human TRB-1 (over-expressed in E. Coli)


Specificity: epitope mapping in the A/B domain hTRB-1 at amino acids 1-101 -reacts with the unliganded, liganded, and DNA binding forms of TR.


Use: -immunoprecipitation: immunoprecipiates 52kDa and 55kDa proteins, representing the products of two alternate start sites of TRB-1

-western blot:detects recombinant TRB-1 which was expressed in E. Coli

-gel shift assay

-immunocytochemistry:recgnizes the transfected and endogeneous T3 nuclear receptor in intact GH3 cell. Cytoplasmic staining and nuclear staining is seen when high levels of the transfected recpetor are expressed.


Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.


References: -K-H Lin, et al. Endocrinology 128(5):2601-2609, 1991

Precautions: For In vitro research Use Only.


-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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