rev:      February 25, 2004

HOME (index page)

Return (master antibody page)

Return  (alphabetical antibody list)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


THROMBIN & Thrombin Receptor Antibodies

matched pair abs (includes coating antibody  and HRP-conjugated detecting ab, sample protocol)

suitable for 5 X 96 wells

cat#RDI-TATEIAKT         Human Thrombin-Antithrombin complex  $750.00

cat#RDI-THCIIEIAKT      Human Thrombin-HCII Complex              $750.00

cat#RDI-ATIIEIAKT         Human Antithrombin III                            $719.00        


DATA SHEET:  Sheep anti- Human Thrombin Polyclonal Antibody IgG

Catalog#: RDI-Thrombinabs  $312.00/10mg     Larger on quotation

Batch#

Package Size: 1.0 (10mg/ml) sheep immunoglobulin fraction in PBS pH 6.5 containing 50% glycerol.

Antigen: Purified human alpha-thrombin inactivated with PPACK.

Specificity: By immunoelectrophoresis:reacts only with thrombin and prothrombin.

-only one arc seen with normal plasma, no arc with barium absorbed plasma.

Titer: must be determined with each method

Application: immunoassays for prothrombin and thrombin as well as thrombin inhibitor complexes.

Storage: Store at -10 to -20 DEG C. AVOID Lower temperatures. Do not freeze diluted antibody.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available:human thrombin $188.00/1KU, $375.00/5Ku   cat#RDI-THROMBIN-AG

also:

sheep anti-rabbit thrombin cat#RDI-THROMRBabS  $312.00/10mg

sheep anti-bovine thrombin cat#RDI-THROMBOVabS $312.0010mg

-HRP conjugates on request

-inquire for antibodies to:bovine thrombin, rabbit thrombin, human prothrombin, fibrinogen, fibrin, protein C, Protein S,

Factor V,8,9,10,11,12,13,prekallikren, plasminogen and more....


DATA SHEET: Sheep anti- Human Thrombin Polyclonal Antibody IgG  AFFINITY PURIFIED

Catalog#: RDI-ThrombinPabs  $438.00/0.5mg      Larger on quotation

Package Size: 0.1ml (5mg/ml) sheep immunoglobulin fraction in 10mM HEPES, pH 6.5 containing 0.1M NaCl  and 50% glycerol.

Antigen: Purified human alpha-thrombin inactivated with PPACK.

Purification: purified on immobilized alpha thrombin

Specificity: By immunoelectrophoresis:reacts only with thrombin.

-only one arc seen with normal plasma, no arc with barium absorbed plasma.

Titer: must be determined with each method

Application: immunoassays for thrombin as well as thrombin inhibitor complexes.


Storage: Store at -10 to -20 DEG C. AVOID Lower temperatures. Do not freeze diluted antibody.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


DATA SHEET: Sheep anti-RABBIT Thrombin Polyclonal Antibody IgG

Catalog#: RDI-THROMRBabs   $312.00/10mg

also affinity purified cat#RDI-THROMRBabs-IG    $469.00/0.5mg

HRP conjugated: cat#RDI-THROMRB-HRP           $469.00/200ug

Batch#

Package Size: 1ml (10mg/ml) sheep immunoglobulin fraction in 10mM HEPES pH 7.4 containing 50% glycerol

Antigen: Purified RABBIT alpha-thrombin inactivated with PPACK.

Specificity: By immunoelectrophoresis:reacts only with thrombin and prothrombin. -only one arc seen with normal rabbit plasma.

Titer: must be determined with each method

Application: immunoassays for prothrombin and thrombin as well as thrombin inhibitor complexes.

Storage: Store at -10 to -20 DEG C. AVOID Lower temperatures. Do not freeze diluted antibody.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available: sheep anti-rabbit antithrombin III


DATA SHEET: Sheep anti-Bovine Thrombin Polyclonal Antibody IgG

Catalog#: RDI-THROMBOVabs  $312.00/10mg

also affinity purified cat#RDI-THROMBOVabs-IG $469.00/0.5mg

HRP conjugated: cat#RDI-THROMBOV-HRP $469.00/200ug

Batch#

Package Size: 1.23ml (8.14mg/ml) sheep immunoglobulin fraction in 10mM HEPES pH 7.4 containing 50% glycerol

Antigen: Purified BOVINE alpha-thrombin inactivated with PPACK.

Specificity: By immunoelectrophoresis:reacts only with thrombin and prothrombin. -only one arc seen with normal bovine plasma.

Titer: must be determined with each method

Application: immunoassays for prothrombin and thrombin as well as thrombin inhibitor complexes.

Storage: Store at -10 to -20 DEG C. AVOID Lower temperatures. Do not freeze diluted antibody.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available:

BOVINE THROMBIN cat#RDI-THROMBOV-AG     $312.00/5K units

Bovine Prothrombin cat#RDI-PRTHOMBOV-AG      $250.00/10mg (100units)


Mouse anti-PAR-1 (Thrombin Receptor) Human/Mouse

cat# RDI-PAR1abm        $440.00/50ug

       RDI-PAR1abm-1     $750.00/150ug

Presentation: 50ug (0.2ml) or 150ug (0.6ml) at 0.25 mg/ml in 50%glycerol,20mM sodium phosphate pH 7.5,150mM NaCl,1.5mM NaN3,1 mg/ml BSA.

Isotype: mouse IgG1

Immunogen: aa 33-153 from human thrombin receptor

Use: western blot (1ug/ml) positive control 50ug/lane K-562 cell lysate. Bad 47kda .Optimize dilution for each use.
         -not tested for other applications


Reactivity: human,mouse (others not tested)

Storage: Store at -20 °C. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

General ref: -Vu, T.-K.H., et al. 1991 Cell 64:1057-1068

                     -Nelken, N.A., et al. 1992 J Clin Invest 90:1614-1621

                     -Niclou, S., et al. 1994 Cell Mol Biol 40:421-428

                    -Coughlin, S.R. 1999 Proc Natl Acad Sci 96:11023-11027

Backgound:Thrombin is a Ser protease that regulates the activity of many cell types including the activation of platelets after vascular injury.Thrombin functions are mediated by G-protein-coupled protease-activated receptors that are homologous to substance P and thyrotropin receptors.Thrombin receptors (PAR1,PAR3,and PAR4)are seven transmembrane domain proteins with large N-terminal exodomains that contain a thrombin cleavage site (LDPR/S).Thrombin binds to the exodomain and cleaves the peptide bond between Arg-41 and Ser-42.This unmasks a new N-terminus with the sequence SFLLRN that act s as a tethered ligand.SFLLRN binds to the body of the thrombin receptor leading to irreversible activation.Both phosphorylation of the thrombin receptor and internalization may uncouple downstream signaling.PAR1 protein is expressed in vascular endothelial cells,smooth muscle cells,and macrophages,while PAR1 mRNA is expressed at higher levels in neonatal rat brain and lower levels in skeletal muscle,liver,and kidney.Thus,G-protein signaling via thrombin receptors,like PAR1,may be important for a diverse array of cellular functions,such as platelet activation and neural development.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

-copyright by owner For In Vitro Research Use Only

Recommend western blot control:

K-562 Cell Lysate. Prepared from a human chronic myelogenous leukemia cell line. Do not boil or dilute.

cat#RDI-K562/CPX $250.00/500ug vial

cell lysate is provided as 500 micrograms in 500ul (1mg/ml) in SDS-PAGE buffer (62 mM Tris pH 6.8, 2% SDS, 0.9% B- mercaptoethanol, 0.003% bromophenol blue and 5% glycerol . Use approx 10-20ul per lane with enhanced chemiluminescence detection.


DATA SHEET: Human Alpha Thrombin Antigen

Catalog#: RDI-Thrombin-AG $188.00/1KU   $375.00/5KU

Bulk quotes on request

Batch# sample data

Package Size: 5000 units (1.52mg) in 0.469ml of 50mM Sodium Citrate/0.2M NaCl/0.1% PEG8000/pH6.5

Units: approx 3281 NIH u/mg, 10663 NIH u/ml

Isolation: This human factor alpha thrombin was purified from human plasma that was tested and found negative for HIV-1, HIV-2, HBSAg and HCV.Donors are screened for CJD (Creutzfeldt- Jakob Disease)

Purity: The human thrombin was activated from homogeneous human prothrombin by activation with Factor Xa, Factro Va and phospholipid. These activating enzymes were removed after activation. Complete activation was observed on 10% SDS-PAGE gels.

Storage: Store at -80 DEG C or lower. AVOID FREQUENT FREEZE THAW CYCLES.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


All antibodes are for In Vitro Research Use Only-Not for Use in Diagnostics

-copyright by owner (s)


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return  (alphabetical antibody list)

Ordering terms