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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Taurine TRANSPORTER  (TAURT)  

see also related antibodies to Vesicular Monoamine Transporters, Acetylcholine, Creatine, Norepinehrine, Proline, Serotonin and Taurine Transporter


Rabbit anti-Rat Taurine Transporter Polyclonal Antibody   (IgG fraction)

Rat/Human/Mouse/Canine

cat# RDI-TAURT1abrX      $500.00/vial

Presentation: 100ug affinity pure antibody in 1.0ml (1mg/ml) 100mM Tris pH7.5 with 0.2% BSA and 0.05% sodium azide.-At first thaw or on receipt, tighten cap,centrifuge for 1 minute at 1000rpm to concentrate antibody in vial. Recommned making any further dilutions in buffer containing 0.2% BSA and 0.05% sodium azide.

Purification: peptide affinity purified.

Antigen: A 20 amino acid peptide sequence specific for the rat taurine transporter, TAUT-1 (c terminal, cytoplasmic)

Reconstitution: All peptide/antibody received in solution or reconstituted from Lyophilized vials should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw.

Specificity: The antigen has 100% sequence homology to mouse and rat and 90% homology to human and canine and 78% with bovine. The peptide shows no significant homology with other known peptides.

USE: Western Blotting (1-10 ug/ml for affinity pure using Chemiluminescence technique).

ELISA (1:10K; using 50-100 ng of control peptide/well).

Histochemistry & Immunofluorescence:Not tested, We recommend the testing of affinity purified antibody at 2-20ug/ml on acetone fixed frozen sections or pfa fixed tissues.

Background:

Taurine (2-aminoethane sulfonic acid), a beta-amino acid with a negatively charged sulfonic group is one of the most abundant amino acid in the brain. It is particularly enriched (milimolar conc.) in excitable tissues, particularly those that generate oxidants. Taurine is produced by cysteine metabolism and obtained from diet as well. It is chemically inert molecule with a long turnover rate. It is not incorporation into proteins. It is thought to pay a major role in osmoregulation. It is also postulated to play a critical role in neurotransmission, neuromodulation, and membrane stabilization, neuronal differentiation, migration, synaptogenesis, and development. Taurine is transported to various target tissues by Na-dependent high affinity uptake system carried out two distinct membrane transporter proteins: a high affinity (Km 40 uM) taurine transporter (TAU-1 or TAUT-1) (1) and a beta-alanine and taurine transporter protein (Km 4.5 uM) known as TAUT-2 (2). Both TAUT-1/2 share significant sequence homology with the GABA and Glycine transporters that are characterized by the presence of 12 transmembrane domains. Taurine transporters are found in brain, retina and many other tissues.

General References:

Smith, K.E. et al. (1992) Mol. Pharmacol. 42, 563; Liu, Q.R. et

al. (1993) 268, JBC, 2106; Uchida, S. et al., (1992) PNAS USA 89,

8230; Jhiang, S.M. et al., (1993) FEBS Lett. 318, 139; (2). Liu

Q-R et al (1992) PNAS 89, 12145; Pow et al (2002) Glia 37, 153.

control/blocking peptide: cat#RDI-TAURTab-CP $188.00/100ug

(lyophilized from 0.1ml (1mg/ml PBS pH 7.4 + 0.05% sodium azide)

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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