rev:   February 2, 2005

HOME (index page)

Return  (alphabetical antibody list)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Surfactant abs (see monoclonals and polyclonal below)
Mouse anti-Surfactant Protein A

cat# RDI-SURFAabm     $375.00/0.1ml

       RDI-SURFAabm1   $812.00/0.5ml

Presentation: Tissue culture supernatant with 0.09% sodium azide

Immunogen: Surfectant apoproteins isolated from lung lavages of patients with alveolar proteinosis

Isotype: mIgG2b

Clone: PE10

Mol. Wt. of Antigen: 70kDa. also detects 34, 36, 37 and 62 kDa species

Reactivity: human (others not tested)

Uses: -histochemistry ( paraffin sections) 1:100-1:300 30 min RT

-no special pretreatment needed

-not tested for other applications

Positive Control: lung adenocarcinomas

Localization: Cytoplasmic granular

Background: Pulmonary surfactant apoproteins are located in type II pneumocytes of the lung play role in reducing surface tension of alveolar interface. These are highly specific to lungs and are also found in many bronchioalveolar carcinomas and adenocarcinomas of lung.

Key References:

1. Kuroki Y, et al. (1985) Biochim Biophys Acta, 836:201-209.

2. Shimizu H, et al. (1989) Tohuku J Exp Med, 157:269-278.

3. Mizutani Y, et al. (1988) Cancer, 61:532-537.

4. Nicholson A G, et al. (1995) Histopathol, 27:57-60.

For Research Use Only


Mouse anti-MATURE-Surfactant Protein B

cat# RDI-SURFBMabm       $438.00/100ug

       RDI-SURFBMabm-1    $719.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml order cat#RDI-SURFBMabm-X $750.00/200ug

Immunogen: recombinant surfactant protein B

Isotype: mIgG2ak

Clone: SPB02

Reactivity: human (others not tested)

Uses: -histochemistry (frozen and paraffin sections) 2-10ug/ml -paraffin sections require boiling tissue sections in 10mM citrate buffer pH6.0 for 10-20min followed by cooling at RT for 20 minutes. approx 1:50-1:100 60 minutes RT

-immunofluorescence

-Western blot-not tested


Positive Control: lung adenocarcinomas

Localization: Cytoplasmic

Background: Recognizes a protein of 8-9kDa, identified as mature Surfactant Protein B (SP-B). This monoclonal is specific to mature SP-B. ProSP-B is glycosylated in the Golgi apparatus and undergoes carboxy- and amino-terminal proteolysis by a cathepsin D-like protease. Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8- 9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B.

For Research Use Only


Mouse anti-PRO-Surfactant Protein B

cat# RDI-SURFBabm         $438.00/100ug

        RDI-SURFBabm-1    $719.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request)   concentration 200ug/ml

Immunogen: recombinant pro-surfactant protein B

Isotype: mIgG

Clone: SPB01

Reactivity: human and rat (others not tested)

Uses: -histochemistry (frozen and paraffin sections) 2-10ug/ml -paraffin sections require boiling tissue sections in 10mM citrate buffer pH6.0 for 10-20min followed by cooling at RT for 20 minutes. approx 1:50-1:100 60 minutes RT

-immunofluorescence

-Western blot-not tested

Positive Control: lung adenocarcinomas

Localization: Cytoplasmic

Background: Recognizes a protein of 42-46kda, identified as PRO-Surfactant Protein B   (SP-B),, Shows no reaction with th emature SP-B (8-9kda). ProSP-B is   glycosylated in the Golgi apparatus and undergoes carboxy- and   amino-terminal proteolysis by a cathepsin D-like protease.   Pulmonary surfactant is a complex mixture of phospholipids and   proteins that is secreted from type II cells in alveoli and   reduces the surface tension at the alveolar air-liquid interface,   providing alveolar stability necessary for normal ventillation.   Four distinct proteins isolated from pulmonary surfactant   are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and   SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8- 9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary   adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of   the lung and nonpulmonary adenocarcinomas do not express SP-B.

For Research Use Only



Mouse anti-Surfactant D (Human/Rat):biotin

biotin conjugated cat#RDI-T3203X    $688.00/200ug

-clone IIE11, mIgG1

-conformational antigen-SP-D)

-Clone IIE1 Mab is useful for staining rat and human surfactant D in paraffin embedded and frozen tissue sections. It stains alveolar type II but not type III cells, alveolar macrophages and two types of Clara cells of which one subpopulation is also positive for SP-A. Clone IIE1 Mab also detects extra-cellular deposits of SP-D.

-suitable for histochemistry on frozen and paraffin sections, elisa and western blots

-ref: M Kasper et al, Exp Lung Res 21, 577 (1995)


Mouse anti-Surfactant D (Human/Rat)

-cat#RDI-T3204X   $660.00/200ug

-clone VIF11, mIgG1

-lectin domain-SP-D)

-Clone VIF11 Mab is useful for staining rat and human surfactant D in paraffin embedded and frozen tissue sections. It stains alveolar type II but not type III cells, alveolar macrophages and two types of Clara cells of which one subpopulation is also positive for SP-A. Clone VIF11 does not cross react with SP-A.  

-suitable for histochemistry on frozen and paraffin sections, elisa and western blots

-ref: M Kasper et al, Exp Lung Res 21, 577 (1995)

-also available biotin conjugated cat#RDI-T3205X $688.00/200ug


RABBIT ANTI-HUMAN PRO-SURFACTANT PROTEIN C   (proSP-C) POLYCLONAL ANTIBODY

CAT# RDI-SURFCPabr2   $440.00

Package: 100uL purified immunoglobulin in PBS (no preservative)

IMMUNOGEN: Recombinant fusion protein containing GST and a.a. 1-20 from the N-terminal of the human SP-C proprotein (expressed in E.coli

REACTIVITY: Human and mouse (others not tested)

SPECIFICITY: Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.).

APPLICATIONS: Immunohistochemistry: 1:2,000-1:4,000 on human tissue with and without antigen retrieval (see below and 1:1,000-1:2,000 on adult and 1:500-1:1,000 on fetal mouse lung . Immunoblot: 1:500-1:1,000 (best if not reduced) ELISA: 1:500-1:1,000

Optimal dilutions must be determined by the end user.

STORAGE: Maintain at -20ºC in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.

For Research Use Only

REFERENCES:

Clark, J., et al., PNAS.USA (1995) 92:7794-7798.

Vorbroker, D.K, et al., Am. J. Physiol. (1995) 268 (Lung. Cell Mol.Physiol. 13):L647-656.

Vorbroker, D.K, et al., Am. J. Physiol. (1995) 269 (Lung. Cell Mol.Physiol.13):L727-733.

Zhou, L., et al., Dev. Biol. (1996) 175:227-238.

Zhou, L., et al., J. Histochem.Cytochem. (1996) 44:1183-1193.

Jain-Vora, S., et al., Am. J. Respir. Cell Mol. Biol. (1997) 17:1-11.

Reed, J., et al., Am. J. Physiol. (1995) 273 (Lung. Cell Mol. Physiol.17):L2-11.

Zsengeller, Z.K., et al., Hum. Gene Ther. (1997) 8:1331-1344.

Morotti, R.A., et al., Human Pathology. (1999) 30:618-625.

Tokieda, K., et al., Am. J. Respir. Cell and Mol. Biol. (1999)21:463-472.

Nogee, L.M., et al., Am. J. Respir. Crit. Care Med. (2000)161:973-981.

IMMUNOHISTOCHEMISTRY

Note: Note: The enzymatic reaction product was enhanced with nickel cobalt to give a black precipitate. Endogenous peroxidase was quenched for 15 min. at RT with 3% H2O2 in methanol. Sections were blocked with 2% normal goat serum in PBS with 0.2% Triton for 2 hours at RT before incubating overnight at 4..C with the properly diluted antibody.  . The sections were then washed 6 times, 5 min. each, in PBS with 0.2% Triton and then incubated for 30 min. at RT with biotinylated goat anti-rabbit antibody properly diluted in the blocking solution. Sections were incubated with the ABC complex diluted in blocking solution for 30 min. at RT; NiDAB in 0.1M acetate buffer for 4 min. at RT; Tris Cobalt for 4 min. at RT; and counterstained with nuclear fast red for 2 min. at RT.

Controls included (1) formalin-fixed, paraffin-embedded, surgical samples of human pediatric and adult lung immunostained at the recommended dilutions or age-matched controls from wild-type, FVB/N, fetal and embryonic mouse pups and (2) omission of the antibody to check for endogenous biotin and peroxidase activity, as well as nonspecific binding of the secondary antibody.

Notes:

1) Dilutions used are for normal (i.e. uninjured) adult mouse lung and have been used on FVB/N, Balb/c, nu/nu and SCID mice. The use of antigen retrieval systems to enhance immunostaining for this antibody is not necessary in the mouse lung and may actually increase background staining.

For research use only; not for use as a diagnostic.


RABBIT ANTI-HUMAN SURFACTANT PROTEIN A (SP-A)   POLYCLONAL ANTIBODY

CAT# RDI-SURFAabr   $438.00/100ul

Presentation: 100ul in PBS (no preservative)

IMMUNOGEN: Human SP-A (deglycosylated, beta-eliminated) purified from human alveolar proteinosis material.

SPECIFICITY: Reacts strongly with both glycosylated and unglycosylated isoforms of SP-A. Immunostains alveolar Type II cells, macrophages, and alveolar proteinosis material in both human proteinosis and in animal models of alveolar proteinosis.

APPLICATIONS: Immunohistochemistry: 1:4,000-1:8,000 with and without antigen retrieval (see protocol on back) Immunoblot: 1:1,000-1:5,000 ELISA: 1:500-1:1,000 Optimal dilutions must be determined by the end user.

SPECIES REACTIVITY: Human, primate and mouse.

STORAGE: Maintain at -20ºC in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.

REFERENCES:

Stahlman, M.T. et al., Pediatr. Res. (1992) 31:364-371.

Khoor, A., et al., J. Histochem. Cytochem. (1994) 42:1187-1199.

Coalson, J.J., et al., Am. J. Respir. Crit. Care Med. (1995) 151:854-866.

Jennings, V.M, et al., Am. J. Respir. Cell Mol. Biol. (1995) 13:297-306.

Vorbroker, D.K, et al., Am. J. Physiol. (1995) 268 (Lung. Cell Mol. Physiol. 13):L647-656

Wert, S.E., et al., Am. J. Respir. Crit. Care Med. (1994) 149:A244.

Wert, S.E., et al., Pediatr. Res. (1996) 39:355A.

Wert, S.E., et al., Pediatr. Res. (1995) 37:356A.

Morotti, R.A., et al., Human Pathology. (1999) 30:618-625.

Nogee, L.M., et al., Am. J. Respir. Crit. Care Med. (2000) 161:973-981.

For research use only; not for use as a diagnostic.

IMMUNOHISTOCHEMISTRY

Note: The enzymatic reaction product was enhanced with nickel cobalt to give a black precipitate. Endogenous peroxidase was quenched for 15 min. at RT with 3% H2O2 in methanol. Sections were blocked with 2% normal goat serum in PBS with 0.2% Triton for 2 hours at RT before incubating overnight at 4..C with the properly diluted antibody. The sections were then washed 6 times, 5 min. each, in PBS with 0.2% Triton and then incubated for 30 min. at RT with biotinylated goat anti-rabbit antibody properly diluted in the blocking solution. Sections were incubated with the ABC complex diluted in blocking solution for 30 min. at RT; NiDAB in 0.1M acetate buffer for 4 min. at RT; Tris Cobalt for 4 min. at RT; and counterstained with nuclear fast red for 2 min. at RT. Controls included (1) formalin-fixed, paraffin-embedded, surgical samples of human pediatric and adult lung immunostained at the recommended dilutions and (2) omission of the antibody to check for endogenous biotin and peroxidase activity, as well as nonspecific binding of the secondary antibody.


RABBIT ANTI-MOUSE SURFACTANT PROTEIN D (SP-D)   POLYCLONAL ANTIBODY

CAT#: RDI-MSURFDabr   $438.00

Presentation 100ul diluted rabbit serum (no preservative)

IMMUNOGEN: Mouse SP-D

SPECIFICITY: Reacts strongly with mouse SP-D.( also human) other species not tested)

APPLICATIONS: -Immunoblot: 1:1,000-1:5,000 (main band approx 43kda)

(on mouse lavage fluid and purified mouse surfactant D, main band at 42kda and another non- specific band at higher MW which is removed when tested with antibody preabosrbed lung homogenates from SP-D null mice)

ELISA: 1:1,000-1:5,000

Optimal dilutions must be determined by the end user.

STORAGE/HANDLING: Maintain at -20ºC in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles

For research use only; not for use as a diagnostic.-please request by email antibodies for other surfactant proteins


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return  (alphabetical antibody list)

Ordering terms