rev:   November 3, 2003

HOME (index page)

Return (master antibody page)

Return  (alphabetical antibody list)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Stat ANTISERA (inquire for Stat 6 and other monoclonals)
Mouse anti-STAT2 Monoclonal ab

cat# RDI-STAT2abm         $438.00/vial 50ug

       RDI-STAT2amb-1     $750.00/vial 150ug

Background: In response to IFN-alpha treatment, Stat1A, Stat1B and Stat2 become tyrosine-phosphorylated and migrate to the nucleus where they join a 48 Kda DNA Binding protein and subsequently direct the transcription at IFN-alpha responsive elements. in IFN-gamma treated cells, STAT1A (but not Stat2) becomes phosphorylated and forms a dimer. It then enters the nucleus and binds to the IFN-gamma activated site (GAS) element in order to direct IGN-G activated transcription. Stat2 phosphorylation can occur independently of Stat1A, but phosphorylated STAT2 does appear to be dependent on its interaction with STAT1A for efficient nuclear localization and/or stability.

isotype: mIgG2a

package: 50ug or 150ug purified antibody (0.25mg/ml) in a 50% glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

antigen: a 21.1 kDa fragment corresponding to amino acids 1-176 of human Stat2

USe: Suitable for western blot on human and chicken, using approx 1 ug/ml ab on total cell lysate. Major band at MW 113kDa. Also suitable for immunofluorescence and immunoprecipitation (native conditions).

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: Improta, T. et al. (1994) Proc. Natl Acad Sci. 91:4776

                   Ihle, J.N. et al (1994) Trends in Biochem Sci 19:222.

Precautions: For In vitro research Use Only. Not for use in or   on humans or animals or for diagnostics.


Also available: Antibodies to STAT1, Stat3, Stat4 ,Stat5 and Stat6.

Stat sampler kit (10ug each of Stat1, Stat2, Stat3, Stat4, Stat5, Stat6) order cat#RDI-STATX $812.00


Mouse anti-STAT5 Monoclonal ab

cat# RDI-STAT5abm       $438.00/vial 50ug

       RDI-STAT5amb-1    $750.00/vial 150ug

Background: Stat5 (MGF) mRNA expression is highest in mammary gland tissue, and lower levels are found in ovary, thymus, spleen, kidney, lung, muscle and the adrenal gland. Once phosphorylated, Stat5 becomes an essential transcription factoir which binds to the B-casein gene promoter. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation. Treatment of activated Stat5 with a protein tyrosine phosphatase results in the loss of DNA binding activity. the cDNA sequence of Stat5 (MGF) has recently been isolated, and the predicted amino acid sequence shows similarity to Stat1 (Stat91/84) and Stat2(Stat113) of the ISGF3 protein complex.

isotype: mIgG2a

package: 50ug or 150ug purified antibody (0.25mg/ml) in a 50% glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

antigen: a 22.8 kDa fragment corresponding to amino acids 451-649 of sheep Stat5.

USe: Suitable for western blot on human ,dog, rat and mouse using approx 1 ug/ml ab on total cell lysate. Major band at MW 92kDa. Also suitable for immunofluorescence .

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: Gouilleus, F. et al, (1994) EMBO. J. 13:4361

                  Wakao, H. et al (1994) EMBO J. 13:2182.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-STAT1 (C terminal) Monoclonal ab

     cat# RDI-STAT1Cabm       $438.00/vial 50ug

            RDI-STAT1Cabm-1    $750.00/vial 150ug

isotype: mIgG2b

package: 50ug or 150ug purified antibody (0.25mg/ml) in a 50% glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

antigen: fragment corresponding to amino acids 592-731 of human Stat1

USe: Suitable for western blot on human ,dog, rat, mouse and chick using approx 1-0.1ug/ml ab on total cell lysate. Positive control A431 cells. Major bands at MW 84/91kDa. Also suitable for immunofluorescence and immunoprecipitation (native or denatured conditions)

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: GAFFEN, S. ET AL. 1995. PROC NATL ACAD SCI USA 92:7192-7196

                   Tsukada, J. et al 1996. Mol Cell Biol 16:2183-2194

                   Nakamura N et al. 1996 J. Biol Chem 271:19483-19488

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Mouse anti-STAT1 (N terminal) Monoclonal ab

cat# RDI-STAT1Nabm             $438.00/vial 50ug

        RDI-STAT1Nabm-1         $750.00/vial 150ug

        RDI-STAT1Nabm-HR (Discontinued)    $500.00/50ug HRP conugated

       RDI-STAt1Nabm-HRX   $812.00/150ug HRP conjugated

isotype: mIgG1

package: 50ug or 150ug purified antibody (0.25mg/ml) in a 50% glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

antigen: fragment corresponding to amino acids 1-194 of human Stat1

USe: Suitable for western blot on human ,dog, rat, mouse ,chick and frog using approx 1-0.1 ug/ml ab on total cell lysate (positive control A431 cells). Major bands at MW 84/91kDa. Also suitable for immunofluorescence and immunoprecipitation (native or denatured conditions)

Main REF: Gobert S. et al, 1996 EMBO J 15:2434-2441

                   Wu, Y et al, 1996 J. Biol. Chem 271:13023-13032

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Rabbit anti-STAT1 (N terminal) ab

cat# RDI-STAT1Nabr       $438.00/vial 50ug

       RDI-STAT1Nabr-1     $750.00/vial 150ug

isotype: mIgG1

package: 50ug or 150ug purified antibody (0.25mg/ml) in a 50% glycerol, 20mM sodium phosphate pH7.5, 150mM NaCl, 1mg/ml BSA and 1.5mM sodium azide.

antigen: fragment corresponding to amino acids 1-194 of human Stat1

USe: Suitable for western blot on human ,dog, rat, mouse ,chick and frog using approx 1ug/ml ab on total cell lysate (positive control human fibroblast cells). Major bands at MW 84/91kDa. Also suitable for immunofluorescence and immunoprecipitation (native or denatured conditions)

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


All antibodes are for In Vitro Research Use Only-Not for Use in Diagnostics

-copyright by owner (s)


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return  (alphabetical antibody list)

Ordering terms